The histone deacetylase (HDAC) inhibitor valproic acid reduces ethanol consumption and ethanol-conditioned place preference in rats

Ameri, Mouza Al; Mansouri, Shamma Al; Maamari, Alyazia Al; Bahí, Amine

doi:10.1016/j.brainres.2014.07.051

Cited by 25 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, pharmacological treatments are able to alter the activity of HDACs. For instance, valproic acid, which is used to treat epilepsy and bipolar disorder, is an inhibitor of HDACs (Al Ameri et al , ). In the final study, we aimed to evaluate the gene expression profiles of Hdacs in peripheral blood in vivo .…”

Section: Discussionmentioning

confidence: 99%

Nalmefene is effective at reducing alcohol seeking, treating alcohol‐cocaine interactions and reducing alcohol‐induced histone deacetylases gene expression in blood

Calleja‐Conde

Echeverry‐Alzate

Giné

et al. 2016

British J Pharmacology

View full text Add to dashboard Cite

BACKGROUND AND PURPOSEThe opioid antagonist nalmefene (selincro®) was approved for alcohol-related disorders by the European Medicines Agency in 2013. However, there have been no studies regarding the effectiveness of nalmefene when alcohol is used in combination with cocaine. EXPERIMENTAL APPROACHUsing operant alcohol self-administration in Wistar rats and qRT-PCR, we evaluated (i) the dose-response curve for s.c. and p.o. nalmefene; (ii) the effects of nalmefene with increasing concentrations of alcohol; (iii) the efficacy of nalmefene on cocainepotentiated alcohol responding; and (iv) the gene expression profiles of histone deacetylases (Hdac1-11) in peripheral blood in vivo and in the prefrontal cortex, heart, liver and kidney post mortem. KEY RESULTSS.c. (0.01, 0.05, 0.1 mg·kg À1 ) and p.o. (10, 20, 40 mg·kg À1 ) nalmefene dose-dependently reduced alcohol-reinforced responding by up to 50.3%. This effect of nalmefene was not dependent on alcohol concentration (10, 15, 20%). Cocaine potentiated alcohol responding by approximately 40% and nalmefene (0.05 mg·kg À1 ) reversed this effect of cocaine. Alcohol increased Hdac gene expression in blood and nalmefene prevented the increases in Hdacs 3, 8, 5, 7, 9, 6 and 10. In the other tissues, alcohol and nalmefene either did not alter the gene expression of Hdacs, as in the prefrontal cortex, or a tissueHdac-specific effect was observed. CONCLUSIONS AND IMPLICATIONSNalmefene might be effective as a treatment for alcohol-dependent patients who also use cocaine. Also, the expression of Hdacs in peripheral blood might be useful as a biomarker of alcohol use and drug response. AbbreviationsHdac, histone deacetylase; qRT-PCR, quantitative RT-PCR

show abstract

Section: Discussionmentioning

confidence: 99%

Nalmefene is effective at reducing alcohol seeking, treating alcohol‐cocaine interactions and reducing alcohol‐induced histone deacetylases gene expression in blood

Calleja‐Conde

Echeverry‐Alzate

Giné

et al. 2016

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…Voluntary ethanol consumption and preference occurred under the two-bottle, free-choice paradigm, as previously described [29,36,40,41,[46][47][48][49][50][51][52][53]. In brief, the two 10 mL pipettes, one containing tap water and the other containing increasing concentrations of ethanol (2.5, 5, 10, 15 and 20%; v/v), were presented for 24 h/day for 5 consecutive days and their positions (left/right) were interchanged daily to prevent development of side preference.…”

Section: Two-bottle Choice Drinking Testmentioning

confidence: 99%

Nucleus accumbens lentiviral-mediated gain of function of the oxytocin receptor regulates anxiety- and ethanol-related behaviors in adult mice

Bahí

Mansouri

Maamari

2016

Physiology & Behavior

Self Cite

View full text Add to dashboard Cite

“…Approximately 50 μL of tail blood samples were collected at 1, 2, and 3 h after injection, and plasma samples were spun down and stored at − 20°C until further analysis. Plasma alcohol concentrations in milligrams per deciliter were measured using an Ethanol Colorimetric Assay Kit (BioVision Research Products, CA, USA) according to the instructions of the manufacturer and as we described previously (Al Ameri et al 2014;Bahi et al 2013. The area under the blood ethanol concentration-time curve (AUC) between 1 and 3 h was determined for each group.…”

Section: Blood Ethanol Concentrations and Alcohol Elimination Ratementioning

confidence: 99%

No effect of sex on ethanol intake and preference after dopamine transporter (DAT) knockdown in adult mice

Bahí

Dreyer

2018

Psychopharmacology

Self Cite

View full text Add to dashboard Cite

Rationale Dopamine levels are controlled in part by transport across the cell membrane by the dopamine transporter (DAT), and recent evidence showed that a polymorphism in the gene encoding DAT is associated with alcoholism. However, research in animal models using DAT knockout mice has yielded conflicting results. Objectives The present study was planned to evaluate the effects of DAT knockdown in the nucleus accumbens (Nacc) on voluntary ethanol consumption and preference in male and female C57BL/6J mice. Methods For this purpose, animals were stereotaxically injected with DAT siRNA-expressing lentiviral vectors in the Nacc, and using a voluntary, continuous access two-bottle choice model of alcohol, we investigated the importance of accumbal DAT expression in voluntary alcohol intake and preference. We also investigated the effects of DAT knockdown on saccharin and quinine consumption and ethanol metabolism. Results We show that females consumed more alcohol than males. Interestingly, DAT knockdown in the Nacc significantly decreased alcohol intake and preference in both groups, but no significant sex by group interaction was observed. Also, DAT knockdown did not alter total fluid consumption, saccharin or quinine consumption, or blood ethanol concentrations. Using Pearson correlation, results indicated a strong positive relationship between DAT mRNA expression and ethanol consumption and preference. Conclusions Taken together, these data provide further evidence that DAT plays an important role in controlling ethanol intake and that accumbal DAT contributes in the modulation of the reinforcing effects of ethanol. Overall, the results suggest that DAT inhibitors may be valuable in the pharmacotherapy of alcoholism. Keywords Alcoholism. DAT. Dopamine transporter. Ethanol. Lentivirus. shRNA. Two-bottle choice Abbreviations BEC Blood ethanol concentration DAT Dopamine transporter LV Lentiviral vectors Nacc Nucleus accumbens shRNA Short hairpin RNA TBC Two-bottle choice Electronic supplementary material

show abstract

The histone deacetylase (HDAC) inhibitor valproic acid reduces ethanol consumption and ethanol-conditioned place preference in rats

Cited by 25 publications

References 49 publications

Nalmefene is effective at reducing alcohol seeking, treating alcohol‐cocaine interactions and reducing alcohol‐induced histone deacetylases gene expression in blood

Nalmefene is effective at reducing alcohol seeking, treating alcohol‐cocaine interactions and reducing alcohol‐induced histone deacetylases gene expression in blood

Nucleus accumbens lentiviral-mediated gain of function of the oxytocin receptor regulates anxiety- and ethanol-related behaviors in adult mice

No effect of sex on ethanol intake and preference after dopamine transporter (DAT) knockdown in adult mice

Contact Info

Product

Resources

About