The histone deacetylase 1/GSK3/SHAGGY-like kinase 2/BRASSINAZOLE-RESISTANT 1 module controls lateral root formation in rice

Abstract

Lateral roots (LRs) are a main component of the root system of rice (Oryza sativa) that increases root surface area, enabling efficient absorption water and nutrients. However, the molecular mechanism regulating LR formation in rice remains largely unknown. Here, we report that Histone Deacetylase 1 (OsHDAC1) positively regulates LR formation in rice. Rice OsHDAC1 RNAi plants produced fewer lateral roots than wild-type plants, whereas plants overexpressing OsHDAC1 exhibited increased lateral root proliferation by promoting LR primordia formation. Brassinosteroid treatment increased the LR number, as did mutation of GSK3/SHAGGY-like kinase 2 (OsGSK2), whereas overexpression of OsGSK2 decreased the LR number. Importantly, OsHDAC1 could directly interact with and deacetylate OsGSK2, inhibiting its activity. OsGSK2 deacetylation attenuated the interaction between OsGSK2 and BRASSINAZOLE RESISTANT 1 (OsBZR1), leading to accumulation of OsBZR1. Overexpression of OsBZR1 increased LR formation by regulating Auxin/IAA signaling genes. Taken together, the results indicate that OsHDAC1 regulates LR formation in rice by deactivating OsGSK2, thereby preventing degradation of OsBZR1, a positive regulator of LR primordia formation. Our findings suggest that OsHDAC1 is a breeding target in rice that can improve resource capture.

Histone deacetylase 9 regulates disease resistance through fine‐tuning histone deacetylation of melatonin biosynthetic genes and melatonin accumulation in cassava

“…HDAs play vital roles in the negative regulation of transcriptional expression during different developmental processes and environmental stress signaling in plants. [42][43][44][45][46][47] Due to the transcriptional reprogramming by HDAs, the involvement of HDAs in plant stress responses has been widely reported in model plants (Arabidopsis, rice, etc.). [45][46][47][48][49][50] Here, this study not only identifies MeHDA9 as a novel regulator of cassava disease resistance, but also illustrates the direct relationship between MeHDA9-mediated histone deacetylation and melatonin biosynthesis in immune responses.…”

“…[42][43][44][45][46][47] Due to the transcriptional reprogramming by HDAs, the involvement of HDAs in plant stress responses has been widely reported in model plants (Arabidopsis, rice, etc.). [45][46][47][48][49][50] Here, this study not only identifies MeHDA9 as a novel regulator of cassava disease resistance, but also illustrates the direct relationship between MeHDA9-mediated histone deacetylation and melatonin biosynthesis in immune responses.…”

“…HDAs play vital roles in the negative regulation of transcriptional expression during different developmental processes and environmental stress signaling in plants 42–47 . Due to the transcriptional reprogramming by HDAs, the involvement of HDAs in plant stress responses has been widely reported in model plants (Arabidopsis, rice, etc.)…”

Histone deacetylase OsHDA706 increases salt tolerance via H4K5/K8 deacetylation of OsPP2C49 in rice

“…Modulating the function of various HDACs can have different effects on rice growth and development. Overexpression of OsHDA702 increases the number of lateral roots on primary roots (Hou et al, 2022), while oshda703 plants showed pleiotropic phenotypes, including delayed growth, short spikes, erect leaves, and reduced flag leaf angle at the mature stage (Wang et al, 2020). oshda705 and oshda714 mutants also exhibited multiple phenotypes (Xu et al, 2021).…”

“…In plants, HDACs are classified into three main families: RPD3/HDA1 (homologous to yeast Rpd3p [Reduced Potassium Dependency3]), Sir2 (homologous to yeast Sir2p [Silent Information Regulator2]), and HD2 (plant‐specific HDACs) (Pandey et al, 2002). HDACs regulate gene transcriptional patterns during development and in response to biotic and abiotic stresses (Ding et al, 2012; Luo et al, 2012; Bourque et al, 2016; Hou et al, 2022).…”