The histology and histochemical aspects of gills of the flower fish, Pseudophoxinus antalyae

Abstract

Branchial epithelium of Pseudophoxinus antalyae was lined by both a thick stratified epithelium lining gill arches, gill rakers and primary filaments and a thin epithelium lining the lamellae. Mucous, chloride and rodlet cells, interspersed between pavement cells, were present in the branchial epithelium. With histochemical procedures for the characterization of glycoconjugates, mucous cells showed a strong positive reaction with Periodic acid-Shiff and Alcian Blue at pH 2.5, although with Alcian Blue at pH 0.5 and pH 1.0 the reaction was much weaker. When the combined Alcian Blue (pH 2.5) - Periodic acid-Shiff reaction was performed, most mucous cells were stained purple, whereas by the combined Aldehyde Fuchsin/Alcian Blue (pH 2.5), most cells showed a positive reaction only to Aldehyde Fuchsin. Methylation/ Alcian Blue (pH 2.5) and Methylation/ Saponification/ Alcian Blue (pH 2.5) methods showed the presence of sulphated and carboxylated glycoconjugates in mucous cells. Mucous cells were also detected to stain all metachromatically with Toluidine Blue.

Gill Morphology in Two Bottom Feeder Mediterranean Sea Fishes: Grey Gurnard Fish (Eutrigla gurnardus, Linnaeus, 1758) and Striped Red Mullet Fish (Mullus barbatus surmuletus, Linnaeus, 1758) by Scanning Electron Microscopy

“…There are great variations in the number of gill arches between the different fish species; in the present study there are three pairs of gills in the striped red mullet, as noted in the puffer fisch (Abumandour & Gewaily), however, the four pairs of gills is the common number in most teleost fishes as in the present investigation in the grey gurnard (Hughes & Grimstone, 1965;Hughes & Morgan;Hossler et al, 1985;Eiras-Stofella et al, 2001;Eiras-Stofella & Fank-de-Carvalho, 2002;Zayed & Mohamed;Cinar et al, 2009;Díaz et al, 2009;Kumari et al, 2009;Kumari et al, 2012;Alsafy;Elsheikh). However, there are five pairs of gills, but the fifth pair is ill developed and devoid from any gill filaments as noted in catfish (Zayed & Mohamed;Kumari et al, 2005;Elsheikh), in contrast, in Senegal sole (Arellano et al, 2004) reported that the fifth pair is well-developed and contains gill filaments.…”

Morphological Studies on the Gills of Puffer Fish (Lagocephalus sceleratus, Gmelin, 1789)

“…There are great variations in the number of gills between the different fish species; in the present study there are three pairs of gills, however, four pairs of gills is the common number in most teleost fishes (Hughes & Grimstone, 1965;Hughes & Morgan;Hossler et al, 1985;Eiras-Stofella et al, 2001;Eiras-Stofella & Fank-deCarvalho, 2002;Zayed & Mohamed;Cinar et al, 2009;Díaz et al, 2009;Kumari et al, 2009Kumari et al, , 2012Alsafy;Elsheikh). However, there are five pairs of gills but the fifth pair is ill developed and devoid from any gill filaments as noted in catfish (Zayed & Mohamed;Kumari et al, 2005;Elsheikh), in contrast, in Senegal sole (Arellano et al, 2004) reported that the fifth pair is well-developed and contain gill filaments.…”

Rodlet cells in epidermal explant cultures of Lepomis Macrochirus

“…; Cinar et al . ). The notable characteristics of these cells are the collection of rodlets as described previously.…”

“…,b; Cinar et al . ; Siderits and Bielek ). The cells are also present in blood, connective tissue, and brain but with lower frequency (Catton ; Smith et al .…”