The Histochemistry and Electron Histochemistry of Chromaffin Tissue

Hopwood, David A.

doi:10.1016/s0079-6336(71)80013-x

Cited by 37 publications

(9 citation statements)

References 325 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glutaraldehyde-fixation followed by post-osmication can fix noradrenaline but cannot fix adrenaline (COUPLAND and HOPWOOD, 1966;HOPWOOD, 1971;COUPLAND, KOBAYASHI and CROWE, 1976). With regard to dopamine, the data published by HOPWOOD (1971) indicates that this method of fixation results in the fixation of only part of dopamine in the tissue.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Autoradiographic Demonstration of a Zonal Distribution of <sup>3</sup>H-Dopamine-Derived Radioactivity in the Mouse Adrenal Medulla Perfusion-Fixed with Glutaraldehyde

Hirano

Kobayashi

Kent

et al. 1977

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

Summary.Localization of 3H-dopamine-derived radioactivity in the chromaffin cells of the mouse adrenal medulla was studied by means of light and electron microscopic autohydrochloride was injected intraperitoneally in seven mice, which were then perfusionfixed from the left ventricle of the heart with 2.5% glutaraldehyde from 15min to 24hrs after injection. Following the post-osmication, dehydration through an ethanol series and Epon embedding, sections of pieces of the adrenal gland and liver were processed for autoradiography using the dipping method.Both A and N cells of the adrenal medulla showed maximum radioactivity at 30min after injection of the isotope. The radioactivity on the A cells was remarkably higher than that on the N cells at 15 to 30min after the injection. However, both types of chromaffin cells contained a similar amount of radioactivity at later time points.It was demonstrated that at all time points examined, both A and N cells were highest in the 3H-dopamine-derived radioactivity in the zone immediately beneath the corticomedullary junction, whereas the radioactivity in the central portion of the adrenal medulla was always lower. No difference was demonstrated between the ultrastructure of the heavily labeled chromaffin cells and that of the lightly labeled cells.No regional difference in the 3H-dopamine-derived radioactivity was demonstrated in the liver lobules.No zonal distribution of radioactivity was demonstrated in autoradiograms of the mouse adrenal medulla prepared after the 3H-leucine (L-leucine-4, 5-3H) injection.These results are consistent with the idea that the chromaffin cells in the zone immediately beneath the cortico-medullary junction of the mouse adrenal gland have a greater dopamine-handling capacity than those in the central portion of the adrenal medulla and that this phenomenon is characteristic of dopamine.Although many peptide-secreting endocrine organs of the body consist of plural populations of parenchymal cells having an individual function secreting different peptide hormones, the functional differentiation in a single population of endocrine cells has not yet been convincingly demonstrated.In the mouse adrenal medulla, since the classical works of BANDER (1950) and ERANKO (1952), two kinds of chromaffin cells have been described. One variety is composed of A cells which secrete adrenaline and the other, N cells, which secrete noradrenaline.However, it remains obscure whether all members of a single type of chromaffin cell always show the same degree of functional activity. 107

show abstract

Section: Discussionmentioning

confidence: 99%

“…With regard to dopamine, the data published by HOPWOOD (1971) indicates that this method of fixation results in the fixation of only part of dopamine in the tissue.…”

Section: Discussionmentioning

confidence: 99%

Autoradiographic Demonstration of a Zonal Distribution of <sup>3</sup>H-Dopamine-Derived Radioactivity in the Mouse Adrenal Medulla Perfusion-Fixed with Glutaraldehyde

Hirano

Kobayashi

Kent

et al. 1977

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

show abstract

“…In cultures of low density (105 cells/ml) many of the cells send out pseudopod-like processes. Chromaffin cells included both norepinephrine and epinephrine types, which can be distinguished by the greater density of the norepinephrine-containing chromaffin vesicles (Hopward, 1971). The cell morphology was typical of that in the intact adrenal medulla.…”

Section: Cell Morphologymentioning

confidence: 99%

Stability of Bovine Adrenal Medulla Cells in Culture

Kilpatrick

Ledbetter

Carson

et al. 1980

Journal of Neurochemistry

240

121

View full text Add to dashboard Cite

The functional stability of primary cultures of adrenal medulla cells was investigated. Isolated cells were prepared by treatment of bovine adrenal glands with collagenase followed by purification on Percoll density gradients and were maintained in Dulbecco's medium containing 10% fetal calf serum. Within 12 h after plating on plastic culture dishes, the cells became firmly attached and exhibited good survival for periods of time up to 3 weeks, as indicated by their morphology using light and electron microscopy, by maintenance of their content of catecholamines, tyrosine hydroxylase, dopamine-beta-hydroxylase, and phenylethanolamine N-methyltransferase, and their ability to respond to secretagogues. During the first 10 days to 2 weeks in culture there was little or no change in any of these parameters. During the 3rd week there were progressive losses of catecholamine and enzyme activities and increased vacuolization of medullary cells. The cells synthesized protein and RNA with no apparent loss in activities over the period studied, but did not incorporate [3H]thymidine into PCA-precipitable material. The cells responded to secretagogues and secretory antagonists similarly to isolated perfused adrenal glands. The studies described here demonstrate that primary cultures of adrenal medulla cells provide an excellent experimental system for obtaining more detailed information on stimulus-secretion coupling and other functional aspects of the adrenal medulla.

show abstract

“…In the present work the adrenal glands of mice which had been injected with [3H]DOPA were processed for light or electron microscopic autoradiography after either fixation in buffered glutaraldehyde followed by osmium tetroxide (this procedure fixes DOPA and noradrenaline in situ; Hopwood, 1971) or fixation in 1-5 % potassium dichromate in 1-5 % glutaraldehyde, pH 7-2, which fixed both adrenaline and noradrenaline (Coupland, Kobayashi & Crowe, 1976). In addition, labelled amines have been extracted from adrenal glands obtained at similar time-intervals after the injection of DOPA and total radioactivity due to catecholamines and the relative proportions of each present at the various times have been determined and correlated with the results of autoradiography.…”

Section: Introductionmentioning

confidence: 99%

OBSERVATIONS ON THE LOCALIZATION OF RECENTLY SYNTHESIZED CATECHOLAMINES IN CHROMAFFIN CELLS AFTER THE INJECTION OF l-[2,5,6-3H]DOPA

Coupland¹,

Kobayashi²,

Kent³

1976

Journal of Endocrinology

View full text Add to dashboard Cite

The fate of tl-[2, 5, 6-3h] dopa, and the intracellular localization of its metabolic products dopamine, noradrenaline and adrenaline, have been determined by the simultaneous use of assay techniques following separation of amines by chromatography and light and electron microscopic autoradiography. During the first 24 h after i.v. or i.p. injection of [3H] DOPA, synthesis of the above catecholamines occurred. Throughout this time the labelled amines were associated with chromaffin granules or immediately adjacent cytosol and not with either the Golgi complex or rough endoplasmic reticulum. Labelling of chromaffin granules occurred simultaneously throughout the cell and there was no evidence of regions containing recently labelled granules and others containing previously charged (older) granules. Adrenaline-storing cells took up [3H] DOPA and its products more rapidly and lost recently synthesized adrenaline more rapidly than noradrenaline-storing cells took up and stored their equivalent amines. This was in keeping with a more rapid turnover of catecholamines in adrenaline-storing elements.

show abstract

The Histochemistry and Electron Histochemistry of Chromaffin Tissue

Cited by 37 publications

References 325 publications

Autoradiographic Demonstration of a Zonal Distribution of <sup>3</sup>H-Dopamine-Derived Radioactivity in the Mouse Adrenal Medulla Perfusion-Fixed with Glutaraldehyde

Autoradiographic Demonstration of a Zonal Distribution of <sup>3</sup>H-Dopamine-Derived Radioactivity in the Mouse Adrenal Medulla Perfusion-Fixed with Glutaraldehyde

Stability of Bovine Adrenal Medulla Cells in Culture

OBSERVATIONS ON THE LOCALIZATION OF RECENTLY SYNTHESIZED CATECHOLAMINES IN CHROMAFFIN CELLS AFTER THE INJECTION OF l-[2,5,6-3H]DOPA

Contact Info

Product

Resources

About