The Histochemical Response of the Human Epidermis to Controlled Injury; Glycogen*†

Lobitz, Walter C.; Holyoke, John B.

doi:10.1038/jid.1954.24

Cited by 86 publications

(20 citation statements)

References 7 publications

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“…5b). A positive periodic acid-Schiff reaction was seen in certain sections of tissue, in the granular layer and midzone of the epidermis, in the amorphous masses in the cutis, and, to some extent, in the thickening hyalin-like walls of the deeper capillaries, but deposits of glycogen were not outstanding as they were in the case of Wells and Rimington.33 Similar staining reactions have been reported recently by Lobitz and Holyoke 18 in specimens of skin subjected to traumatic irritation with adhesive tape. The deposits of glycogen, then, are probably a response to an irritant of indefinite character.…”

supporting

confidence: 63%

Observations on Porphyria Cutanea Tarda

Brunsting¹

1954

Arch Dermatol

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supporting

confidence: 63%

Observations on Porphyria Cutanea Tarda

Brunsting¹

1954

Arch Dermatol

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“…1975;Gibbins 1978;Krawczyk 1971;Lobitz and Holyoke 1954;Mansbridge and Knapp 1987;Odland and Ross 1968;Ordmann and Gillman 1968;Ortonne et al 1981;Sciubba 1977;Viziam et al 1964;Winter 1972). As these studies employed different experimental animals or different kinds of injuries, the histological changes in these observations were not exactly the same.…”

Section: Discussionmentioning

confidence: 94%

Changes in cytokeratin expression in epidermal keratinocytes during wound healing

Watanabe

Osumi

Ohnishi

et al. 1995

Histochem Cell Biol

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In order to investigate the re-epithelialization process during wound healing, the hair on the back of guinea pigs was shaved and then excisional wounds were made through the entire thickness of the skin. Histological changes were observed and changes in the expression of different cytokeratin polypeptides were examined using an immunohistochemical technique. Immunohistochemical study revealed that the proliferating and migrating keratinocytes expressed the same cytokeratins as the basal cells of normal epidermis. In addition, the entire epidermis of fairly remote areas from the edges of the wound where no thickening was observed showed a temporarily abnormal staining pattern. The suprabasal cells in the regenerating epidermis temporarily expressed cytokeratins not only specific for suprabasal cells but also specific for basal cells. The cytokeratins expressed in normal basal keratinocytes were also present in the thickened granular layers. These data indicate that the expression of cytokeratins in the epidermal keratinocytes (even in fairly remote areas from the wound edges) changes during wound healing, that the origin of the migrating keratinocytes from the remaining epidermis seems to be the basal cells in the epidermis, and that the appearance of keratohyalin granules is not related to changes in cytokeratin expression.

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“…Previously, results suggested by Lobitz et al in human skin showed that removal of the epidermis induced proliferation predominantly in basal cells of the SG straight ducts [15]. Subsequent experiments where SG ducts were injured showed an initial migration of lumenal cells after 24 h followed by proliferation of basal cells at 48 h giving rise to new spiraling lumenal cells as well as tongues of prickle cells at both ends of the cut duct [16].…”

Section: Introductionmentioning

confidence: 98%

Label Retaining Cells (LRCs) with Myoepithelial Characteristic from the Proximal Acinar Region Define Stem Cells in the Sweat Gland

et al. 2013

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Slow cycling is a common feature shared among several stem cells (SCs) identified in adult tissues including hair follicle and cornea. Recently, existence of unipotent SCs in basal and lumenal layers of sweat gland (SG) has been described and label retaining cells (LRCs) have also been localized in SGs; however, whether these LRCs possess SCs characteristic has not been investigated further. Here, we used a H2BGFP LRCs system for in vivo detection of infrequently dividing cells. This system allowed us to specifically localize and isolate SCs with label-retention and myoepithelial characteristics restricted to the SG proximal acinar region. Using an alternative genetic approach, we demonstrated that SG LRCs expressed keratin 15 (K15) in the acinar region and lineage tracing determined that K15 labeled cells contributed long term to the SG structure but not to epidermal homeostasis. Surprisingly, wound healing experiments did not activate proximal acinar SG cells to participate in epidermal healing. Instead, predominantly non-LRCs in the SG duct actively divided, whereas the majority of SG LRCs remained quiescent. However, when we further challenged the system under more favorable isolated wound healing conditions, we were able to trigger normally quiescent acinar LRCs to trans-differentiate into the epidermis and adopt its long term fate. In addition, dissociated SG cells were able to regenerate SGs and, surprisingly, hair follicles demonstrating their in vivo plasticity. By determining the gene expression profile of isolated SG LRCs and non-LRCs in vivo, we identified several Bone Morphogenetic Protein (BMP) pathway genes to be up-regulated and confirmed a functional requirement for BMP receptor 1A (BMPR1A)-mediated signaling in SG formation. Our data highlight the existence of SG stem cells (SGSCs) and their primary importance in SG homeostasis. It also emphasizes SGSCs as an alternative source of cells in wound healing and their plasticity for regenerating different skin appendages.

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The Histochemical Response of the Human Epidermis to Controlled Injury; Glycogen*†

Cited by 86 publications

References 7 publications

Observations on Porphyria Cutanea Tarda

Observations on Porphyria Cutanea Tarda

Changes in cytokeratin expression in epidermal keratinocytes during wound healing

Label Retaining Cells (LRCs) with Myoepithelial Characteristic from the Proximal Acinar Region Define Stem Cells in the Sweat Gland

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