The histochemical differences of intestinal gland epithelia in the rat colon with special reference to their glycoconjugates.

Tsuyama, Shinichiro; Suzuki, Shintaro; Murata, Fusayoshi

doi:10.1267/ahc.16.456

Cited by 10 publications

(9 citation statements)

References 32 publications

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“…It has been demonstrated by a variety of carbohydrate stainings that goblet cells of the distal colon elaborate sulfated glycoconjugates in different animals (SHEAHAN and JERVIS, 1976;PODDAR and JACOB, 1981;TSUYAMA et al, 1983). The reaction of non-sulfonic acidic groups of glycoconjugates is obscured by the strong reaction of sulf onic groups in these carbohydrate stainings, and the presence of sialyl residues in goblet cells has been revealed by lectin histochemistry (FREEMAN et al, 1980, CALDERO et al,1988; the present study).…”

Section: Discussionsupporting

confidence: 54%

“…The present authors fundamentally agree with them: vacuolated cells in the mouse distal colon bind LFA throughout all the stages of ontogenesis, indicating the presence of sialyl residues in glycoconjugates. The appearence of increased PAS reactivity in vacuolated cells in the present study after periodic acid-borohydride-potassium hydroxide treatment indicates that certain sialyl residues in glycoconjugates are 0-acetylated or 0-acylated at least in part (REID et al, 1973;CULLING et al, 1974;TSUYAMA et al, 1983;PEARSE, 1985). However, the possibility that the increased PAS reactivity ought to be attributed to the presence of N-acetylhexosamines cannot be ruled out if the potassium hydroxide treatment cleaves the acetyl group of these.…”

Section: Discussionsupporting

confidence: 50%

“…Different results have been reported on the binding of UEA-I in goblet cells of the distal colon. In rats, which have been the most extensively studied, some authors have reported negative reactions (ESSNER et al, 1978;TSUYAMA et al,1983;COLONY and STEELY, 1987), whereas others have noted variable reactivities depending on the sites of the cells or ontogenetic stages (FREEMAN et al, 1980;CALDERO et al, 1988). In the mouse distal colon, goblet cells bind UEA-I and are thus considered to have fucosylated glycoconjugates (BOLAND and AHNEN,1985; the present study).…”

Section: Discussionmentioning

confidence: 56%

“…The paper by TSUYAMA et al (1983) has been, to our knowledge, the only report dealing with glycoconjugates in vacuolated cells. In their report, the cells in the distal colon of adult rats were considered to contain 0-acylated sialic acid in results obtained by periodic acid-thionin Schiff/periodate-borohydride/ potassium hydroxide/periodic acid-Schiff technique for light microscopy and its modification for electron microscopy.…”

Section: Discussionmentioning

confidence: 95%

“…To our knowledge, the only histochemical study to distinguish vacuolated cells from goblet cells has been carried out in the colon of adult rats, where staining patterns of mucins of these two types of cells were apparently different from each other (TSUYAMA et al, 1983). Although many histochemical studies on glycoconjugates during the course of colonic development have been carried out, no special statement has been made as to vacuolated cells (ESSNER et al,1978;FREEMAN et al, 1980;PODDAR and JACOB, 1981;BOLAND and AHNEN, 1985;COLONY and STEELY, 1987;PONGCHAIRERKS et a1., 1987;CALDERO et al, 1988).…”

mentioning

confidence: 99%

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A Histochemical Study on Glycoconjugates in Epithelial Cells in the Distal Colonic Mucosa of Adult and Developing Mice.

Chen

Suzaki

Morino-Kohno

et al. 1993

Archives of Histology and Cytology

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Summary. Glycoconjugates were histochemically studied in the distal colon of developing ICR mice in view of the presence of goblet and vacuolated cells. Alcian blue, high iron diamine and periodic acid-Schiff stainings were performed to characterize glycoconjugates. In addition, two lectins, Ulex europeus agglutinin I and Limax ftavus agglutinin, were applied to detect fucosyl and sialyl residues, respectively. The reactivities to these stainings, noted from day 18 of gestation, did not seem to undergo any major change throughout their development. The present results suggest that: 1) goblet cells secrete sulfated glycoconjugates containing fucosyl and sialyl residues as terminal sugars; 2) vacuolated cells have glycoconjugates containing sialyl residues but no or few fucosyl residues nor sulfonic groups, and certain sialyl residues in the glycoconjugates are probably 0-acetylated or 0-acylated at least in part; and 3) the brush border of absorptive cells contains glycoconjugates sialylated and fucosylated to some extent but rarely sulfated.Since glycoconjugates elaborated by goblet and vacuolated cells differ from each other, one should be fully aware of the presence of these two types of mucinproducing cells in the distal colon.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

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A Histochemical Study on Glycoconjugates in Epithelial Cells in the Distal Colonic Mucosa of Adult and Developing Mice.

Chen

Suzaki

Morino-Kohno

et al. 1993

Archives of Histology and Cytology

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Distribution and changes of glycoconjugates in rat colonic mucosa during development

et al. 1988

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A study was made of the modifications of glycoconjugates in rat colonic mucosa during development. Sections of the caecum, and proximal and distal portions of the colon from Sprague Dawley rats at different stages of development (embryos, fetuses, suckling, weaning and adult rats) were examined. The sections were incubated with a battery of eight fluoresceinated lectins: DBA, SBA, WGA, LFA, PNA, GS-I, UEA-I and Con A. Some sections were treated with neuraminidase, and others were submitted to sequential saponification-neuraminidase treatment prior to incubation with the lectin (WGA, PNA or LFA). The intensity of the fluorescence was evaluated and graded from absent (-) to very positive (4+). Gradual and progressive changes were seen in colonic glycoconjugates during development. These changes revealed a unique developmental pattern for each lectin, which was independent for each cellular compartment (goblet cells, luminal surface and supranuclear region). Local and regional differences, observed between the different colonic sections, were already present from early stages of development. Moreover, our study showed that for several glycoconjugates, the differentiation process in colonic mucosa began in the distal region and continued through to the proximal region, the former being the first to reach the adult pattern. In the caecum, some lectins maintained a fetal pattern throughout all the periods of development up to the adult stage.

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Light-microscopic detection of acidic glycoconjugates with sensitized diamine procedures

Hirabayashi

1992

Histochem J

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To enhance the efficiency and specificity of diamine methods in light microscopy, these methods were sensitized by silver enhancement in combination with trichloro(ethylene) platinate (KTP). The sensitized diamine methods consisted of a diamine (high or low iron diamine: HID or LID), KTP, borohydride reduction (BH) and a physical development (PD) sequence. The new methods have been successfully applied to routinely prepared tissue sections obtained from rat organs, such as salivary glands, stomach, colon, kidney, lung and trachea. In the tissues subjected to the sensitized diamine methods, weakly diamine-stained histological structures exhibited vivid positive reactions. The combined sensitized diamine methods and selective procedures, such as enzyme digestion and chemical modification, have substantiated that these methods were of sufficient efficiency and specificity.

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The histochemical differences of intestinal gland epithelia in the rat colon with special reference to their glycoconjugates.

Cited by 10 publications

References 32 publications

A Histochemical Study on Glycoconjugates in Epithelial Cells in the Distal Colonic Mucosa of Adult and Developing Mice.

A Histochemical Study on Glycoconjugates in Epithelial Cells in the Distal Colonic Mucosa of Adult and Developing Mice.

Distribution and changes of glycoconjugates in rat colonic mucosa during development

Light-microscopic detection of acidic glycoconjugates with sensitized diamine procedures

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