The highs and lows of programmed cardiovascular disease by developmental hypoxia: studies in the chicken embryo

Itani, Nozomi; Salinas, Carlos; Villena, Mercedes; Skeffington, Katie L.; Beck, Chritian; Villamor, Eduardo; Blanco, Carlos E.; Giussani, Dino A.

doi:10.1113/jp274111

Cited by 29 publications

(36 citation statements)

References 115 publications

(296 reference statements)

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“…[7][8][9][10] In 2017, Li et al 11 demonstrated that CoCl 2 -induced hypoxia promoted cell migration and proliferation in placenta-derived mesenchymal stem cells. [7][8][9][10] In 2017, Li et al 11 demonstrated that CoCl 2 -induced hypoxia promoted cell migration and proliferation in placenta-derived mesenchymal stem cells.…”

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confidence: 99%

MALAT1 promoted cell proliferation and migration via MALAT1/miR‐155/MEF2A pathway in hypoxia of cardiac stem cells

Wang

Chen

2018

J of Cellular Biochemistry

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Accumulating evidence revealed that hypoxia contributed to many human diseases, including ischemic myocardium and heart failure (HF). In recent years, the roles of hypoxia in stem cell survival and cardiac biology have been studied extensively. However, the underlying molecular mechanisms remain to be elucidated. As a leading cause of HF, ischemic heart disease was correlated with hypoxia. In this study, we firstly constructed the hypoxia cell model by CoCl2 in cardiac stem cells (CSCs) and found that hypoxia induced the cell proliferation and migration potential in CSCs. Then, we demonstrated that the expression of metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) was promoted in CoCl2‐induced CSCs hypoxia model. Furthermore, we found that knockdown of MALAT1 inhibited the cell proliferation and migration in CoCl2‐induced CSCs hypoxia model. In addition, we revealed that MALAT1 regulated the microRNA‐155 (miR‐155) expression in CSCs under both the normal and hypoxia conditions and further, manipulation of the miR‐155 expression affected the role of MALAT1 in CoCl2‐induced CSCs hypoxia cell model. We then illustrated that miR‐155 regulated the myocyte enhancer factor 2A (MEF2A) expression in CSCs under both the normal and hypoxia conditions and further, changing the expression of MEF2A affected the role of miR‐155. Finally, we demonstrated that MALAT1 regulated the MEF2A expression and exerted its role via modulation of the MALAT1/miR‐155/MEF2A pathway. Taken together, our study illustrated that MALAT1 promoted the cell proliferation and migration in CoCl2‐induced CSCs hypoxia model, acting mechanistically by promoting MEF2A expression via “sponging” miR‐155.

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confidence: 99%

MALAT1 promoted cell proliferation and migration via MALAT1/miR‐155/MEF2A pathway in hypoxia of cardiac stem cells

Wang

Chen

2018

J of Cellular Biochemistry

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“…Several mammalian models have helped to unravel the biology and pathobiology of the ductus arteriosus (DA) and to understand the factors responsible for its closure or patency after birth. However, these models are technically complex and experimental manipulations affect both the mother and the fetus [1,2]. Therefore, there is a need for additional models, addressing these limitations.…”

Section: The Chicken Embryo As a Model For Ductus Arteriosus' Developmentioning

confidence: 99%

New Mediators in the Biology of the Ductus Arteriosus: Lessons from the Chicken Embryo

Sterren

Mohammed

Villamor

2020

Molecular Mechanism of Congenital Heart Disease and Pulmonary Hypertension

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The chicken embryo is an ideal model for the study of new hypotheses on the developmental biology of ductus arteriosus (DA). A unique characteristic of chicken DA is that it is the result of the fusion of two vessels with different embryological origins, morphologies, and functionalities. The pulmonary side (PulmDA) consists almost exclusively of neural crest-derived cells, shows the structure of a muscular artery, and responds to O 2 with contraction whereas the aortic part is of mesodermal origin, shows the morphology of an elastic artery and relaxes in response to O 2. In addition the two parts of the DA show marked differences in responsiveness to other contractile and relaxant agents. In mammals, the most accepted model of O 2-induced DA constriction involves a rise in O 2 modulating the function of the mitochondrial electron transport chain (the sensor), leading to an increased production of H 2 O 2 (the mediator) that causes the inhibition of K V channels (the effector) with Rho kinase acting as another downstream effector of the O 2-sensing system in the DA. In the chicken embryo, we verified the very same pathway, proving a conserved mechanism for O 2 sensing/signaling in mammalian and nonmammalian DA. Moreover, we demonstrated a developmentally regulated response to O 2 , which is restricted to the mature PulmDA and involves parallel maturation of the three components: sensor, mediator, and effectors. Besides O 2 , we used the chicken embryo model to investigate the possible ductal effects of vasoactive mediators such as ceramide, H 2 S, isoprostanes, or platelet-derived vasoactive mediators.

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“…Correctly diagnosing arrhythmia and recognising complex arrhythmia have been signi cant goals in the history of modern electrocardiology [1]. Arrhythmia monitoring is an important type of long-term electrocardiogram (ECG) monitoring that is mainly used to detect arrhythmias, diagnose CVD in the early stages, evaluate anti-arrhythmic treatment, understand the relationship between premature ventricular contraction (PVC) and sudden death, perform electrophysiological studies of arrhythmia, and evaluate drug treatment effects [2][3]. Therefore, arrhythmia monitoring is critical for clinical analysis.…”

Section: Introductionmentioning

confidence: 99%

Automatic Arrhythmia Detection Using One-Dimensional Convolutional Neural Network

Liu¹,

Wang²

2020

Preprint

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Background: Cardiovascular diseases (CVDs) are common diseases that pose significant threats to human health. Statistics have demonstrated that a large number of individuals die unexpectedly from sudden CVDs. Therefore, real-time monitoring and diagnosis of abnormal changes in cardiac activity are critical, as they can help the elderly and patients handle emergencies in a timely manner. To this end, a round-the-clock electrocardiogram (ECG) monitoring system can be developed with the quick detection of an ECG signal, segmentation of the detected ECG signal, and rapid diagnosis of a single segmented ECG beat. In this paper, to achieve the automatic detection and diagnosis of an ECG signal, five common types of ECG signals are used for recognition. For pre-processing the original ECG signal, the dual-slope detection algorithm is proposed and developed. Then, with the pre-processed ECG data, a five-layer one-dimensional convolutional neural network is constructed to classify five categories of heartbeats, namely, a normal heartbeat and four types of abnormal heartbeats. Results: To be able to compare the results of the experiment, the experimental data used in this study are obtained from the open-source MIT-BIH arrhythmia database. This database is authoritative, as each ECG signal cycle is annotated by at least two cardiologists, and abnormal ECG signals are classified into different categories. By comparing the detection and recognition results in this study with the results annotated in the MIT-BIH arrhythmia database, an overall accuracy of 96.20% is achieved in the classification of normal ECG signals and four categories of abnormal ECG signals.Conclusions: This paper provides an accurate method with low computational complexity for 24-hour dynamic monitoring and automated diagnosis of heartbeat conditions. With wearable devices, this method can be used at home for the initial screening of CVDs. In addition, it can perform diagnosis and warning for postoperative patients or patients with chronic CVDs.

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The highs and lows of programmed cardiovascular disease by developmental hypoxia: studies in the chicken embryo

Cited by 29 publications

References 115 publications

MALAT1 promoted cell proliferation and migration via MALAT1/miR‐155/MEF2A pathway in hypoxia of cardiac stem cells

MALAT1 promoted cell proliferation and migration via MALAT1/miR‐155/MEF2A pathway in hypoxia of cardiac stem cells

New Mediators in the Biology of the Ductus Arteriosus: Lessons from the Chicken Embryo

Automatic Arrhythmia Detection Using One-Dimensional Convolutional Neural Network

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