The highly conserved DAD1 protein involved in apoptosis is required for N‐linked glycosylation

Makishima, Tomoko; Nakashima, Torahiko; Nagata-Kuno, Kazue; Fukushima, Kohtaro; Iida, Hiroshi; Sakaguchi, Masao; Ikehara, Yukio; Komiyama, S.; Nishimoto, Tetsuya

doi:10.1046/j.1365-2443.1997.1070303.x

Cited by 50 publications

(48 citation statements)

References 32 publications

(56 reference statements)

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“…This gene, Dad1, is expressed ubiquitously (9), and the coding sequences of Dad1 are highly conserved between species even in organisms that do not have TCR genes, such as yeast (10) or Caenorhabditis elegans (11). The Dad1 protein has been shown to play a role in preventing apoptosis in certain cell types (10,(12)(13) and is also a subunit of the oligosaccharyltransferase enzyme complex that initiates N-linked glycosylation (10,14).…”

Section: The T Cell Receptor Genes (Tcr)mentioning

confidence: 99%

Both CTCF-dependent and -independent Insulators Are Found between the Mouse T Cell Receptor α and Dad1 Genes

Magdinier¹,

Yusufzai

Felsenfeld

2004

Journal of Biological Chemistry

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The T cell rearrangement of the T cell receptor (TCR) genes TCR␣ and ␦ is specifically regulated by a complex interplay between enhancer elements and chromatin structure. The ␣ enhancer is active in T cells and drives TCR␣ recombination in collaboration with a locus control region-like element located downstream of the C␣ gene on mouse chromosome 14. Twelve kb further downstream lies another gene, Dad1, with a program of expression different from that of TCR␣. The ϳ6-kb locus control region element lying between them contains multiple regulatory sites with a variety of roles in regulating the two genes. Previous evidence has indicated that among these there are widely distributed regions with enhancer blocking (insulating) activity. We have shown in this report that one of these sites, not previously examined, strongly binds the insulator protein CCTC-binding factor (CTCF) in vitro and in vivo and can function in an enhancer blocking assay. However, other regions within the 6-kb element that also can block enhancers clearly do not harbor CTCF sites and thus must reflect the presence of a previously undetected and distinct vertebrate insulator activity.

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Section: The T Cell Receptor Genes (Tcr)mentioning

confidence: 99%

Both CTCF-dependent and -independent Insulators Are Found between the Mouse T Cell Receptor α and Dad1 Genes

Magdinier¹,

Yusufzai

Felsenfeld

2004

Journal of Biological Chemistry

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“…Dad1 encodes a protein with antiapoptotic activity (2). Mice bearing Dad1 null mutations die in utero displaying evidence of increased and inappropriate apoptosis (3)(4)(5). Furthermore, Dad1 expression levels are greatly increased in the late stages of thymocyte development and overexpression of Dad1 in peripheral T cells causes hyperproliferation in response to TCR activation (6).…”

mentioning

confidence: 99%

CTCF-Independent, but Not CTCF-Dependent, Elements Significantly Contribute to TCR-α Locus Control Region Activity

Gomos-Klein

Harrow

Alarcón

et al. 2007

The Journal of Immunology

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The mouse TCRα/TCRδ/Dad1 gene locus bears a locus control region (LCR) that drives high-level, position-independent, thymic transgene expression in chromatin. It achieves this through DNA sequences that enhance transcription and protect transgene expression from integration site-dependent position effects. The former activity maps to a classical enhancer region (Eα). In contrast, the elements supporting the latter capacity that suppresses position effects are incompletely understood. Such elements likely play important roles in their native locus and may resemble insulator/boundary sequences. Insulators support enhancer blocking and/or chromatin barrier activity. Most vertebrate enhancer-blocking insulators are dependent on the CTCF transcription factor and its cognate DNA binding site. However, studies have also revealed CTCF-independent enhancer blocking and barrier insulator activity in the vertebrate genome. The TCRα LCR contains a CTCF-dependent and multiple CTCF-independent enhancer-blocking regions whose roles in LCR activity are unknown. Using randomly integrated reporter transgenes in mice, we find that the CTCF region plays a very minor role in LCR function. In contrast, we report the in vivo function of two additional downstream elements located in the region of the LCR that supports CTCF-independent enhancer-blocking activity in cell culture. Internal deletion of either of these elements significantly impairs LCR activity. These results reveal that the position-effect suppression region of the TCRα LCR harbors an array of CTCF-independent, positive-acting gene regulatory elements, some of which share characteristics with barrier-type insulators. These elements may help manage the separate regulatory programs of the TCRα and Dad1 genes.

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“…Tunicamycin has been reported to induce apoptosis in several cultured cell lines (7)(8)(9)(10). However, we could not detect apoptosis in BHK21 cells treated with tunicamycin, although cells treated with tunicamycin died quickly (6). In order to clarify whether loss of N-linked glycosylation causes apoptosis, it is essential to show that the wild-type BHK21 cell line which is parental for the tsBN7 cell line, enters apoptosis by loss of N-linked glycosylation.…”

mentioning

confidence: 77%

“…One of these ts mutants, tsBN7, has a mutation in the DAD1 gene (4) encoding a homologue of S. cerevisiae protein, Ost2p, which is an ⑀-subunit of the S. cerevisiae oligosaccharide transferase (5). Consistently, tsBN7 cells are defective in N-linked glycosylation at the restrictive temperature 39.5°C (6). Since tsBN7 cells enter apoptosis at the restrictive temperature, loss of N-linked glycosylation was thought to cause apoptosis.…”

mentioning

confidence: 99%

Inhibition of N-Linked Glycosylation Causes Apoptosis in Hamster BHK21 Cells

Yoshimi

Sekiguchi

Hara

et al. 2000

Biochemical and Biophysical Research Communications

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The highly conserved DAD1 protein involved in apoptosis is required for N‐linked glycosylation

Cited by 50 publications

References 32 publications

Both CTCF-dependent and -independent Insulators Are Found between the Mouse T Cell Receptor α and Dad1 Genes

Both CTCF-dependent and -independent Insulators Are Found between the Mouse T Cell Receptor α and Dad1 Genes

CTCF-Independent, but Not CTCF-Dependent, Elements Significantly Contribute to TCR-α Locus Control Region Activity

Inhibition of N-Linked Glycosylation Causes Apoptosis in Hamster BHK21 Cells

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