Abstract:An important step towards understanding the replication process in the context of the cell is the quantitative characterization of replication dynamics, including the rate of replication fork progression (i.e. fork velocity) with genomic and temporal specificity in vivo. In this paper, we develop a novel method, lag-time analysis, for measuring replisome dynamics using next-generation sequencing. We provide the first quantitative locus-specific measurements of fork velocity. The measured velocity is observed t… Show more
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