The Hidden Diversity of Diatrypaceous Fungi in China

Abstract

In this study, we investigated the diversity of diatrypaceous fungi from six regions in China based on morpho-molecular analyses of combined ITS and tub2 gene regions. We accept 23 genera in Diatrypaceae with 18 genera involved in the phylogram, and the other five genera are lacking living materials with sequences data. Eleven species included in four genera (viz. Allocryptovalsa, Diatrype, Diatrypella, and Eutypella) have been isolated from seven host species, of which nine novel species (viz. Allocryptovalsa castaneae, A. castaneicola, Diatrype betulae, D. castaneicola, D. quercicola, Diatrypella betulae, Da. betulicola, Da. hubeiensis, and Da. shennongensis), a known species of Diatrypella favacea, and a new record of Eutypella citricola from the host genus Morus are included. Current results show the high diversity of Diatrypaceae which are wood-inhabiting fungi in China.

Figure 3 from: Long S, Liu L, Pi Y, Wu Y, Lin Y, Zhang X, Long Q, Kang Y, Kang J, Wijayawardene NN, Wang F, Shen X, Li Q (2021) New contributions to Diatrypaceae from karst areas in China. MycoKeys 83: 1-37. https://doi.org/10.3897/mycokeys.83.68926

“…Based on phylogenetic analyses, Diatrypella pseudooregonensis was introduced as an 8-spored species of Diatrypella. Our phylogenetic analyses, based on ITS and β-tubulin, agree with the previous studies (Acero et al 2004;Trouillas et al 2011;Mehrabi et al 2015Mehrabi et al , 2016de Almeida et al 2016;Shang et al 2017;Dissanayake et al 2021;Zhu et al 2021). However, several genera are not monophyletic;for example, Cryptosphaeria, Diatrype, Diatrypella, and Eutypa.…”

“…We will discuss its classification status after more strains, more gene sequences and new taxonomic features are collected. Some species of Diatrypella (D. iranensis and D. macrospora) which have polysporous ascus are placed between species of Diatrype, and they are transferred to Diatrype iranensis and Diatrype macrospora by Zhu et al (Zhu et al 2021). Diatrype enteroxantha is often derived from the sister clade of Allodiatrype rather than the Diatrype clade.…”

“…in clade 7 are solitary and scattered, which is distinctly different from widely effuse, flat and slightly convex stromata of Diatrype lancangensis and Diatrype palmicola (Liu et al 2015;Hyde et al 2020b;Zhu et al 2021). And in the recent study, Zhu et al (2021) proposed that the species of Diatrypella in Clade 7 were isolated from Betula spp., it may have host specificity. Because of the above two reasons, we think it is better to classify our strains into Diatrype.…”

“…Phylogenetic analyses were performed by searching homologous sequence data of the family Diatrypaceae in the GenBank database, selected from NCBI and recently published papers (Mehrabi et al 2019;Dayarathne et al 2020;Konta et al 2020;Dissanayake et al 2021;Zhu et al 2021). After the preliminary identification results of the sequences, multiple sequence alignments (ITS and β-tubulin) were aligned using Bi-oEdit v. 7.0 (Hall 1999).…”

Two New Species of Diatrype (Xylariales, Ascomycota) with Polysporous Asci from China

“…Tul., and Eutypella (Nitschke) Sacc., were performed, and suggesting a polyphyletic origin for the five genera [4]. More recently, many diatrypaceous taxa were described and illustrated based on morphological characters and multi-gene phylogenetic analyses [8][9][10][11]18,[22][23][24]. The taxonomy and molecular phylogeny of Diatrype have not been so well studied in China.…”