The Hidden Chemical Cross-Talk Between Roots and Microbes: A Proteomic Approach

De‐la‐Peña, Clelia; Loyola‐Vargas, Víctor M.

doi:10.2174/157016412800786194

Cited by 5 publications

(3 citation statements)

References 168 publications

(257 reference statements)

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“…Due to the demand to increase the productivity, this crop requires the use of large amounts of fertilizers, pesticides, and other chemical inputs, which cause damage to human health and to the environment. Various studies that evaluate the use of microorganisms to replace or decrease the use of toxic inputs in agriculture provide a way to improve crop productivity and increase plant tolerance to stress (De-La-Peña & Loyola-Vargas, 2014). Plants live in association with microbes that can confer positive, negative, or neutral interactions with their host (Schlaeppi & Bulgarelli, 2015).…”

Section: Introductionmentioning

confidence: 99%

Screening of plant growth-promoting bacteria isolated from sugarcane

Bezerra

Takita

Tosta

et al. 2022

Semina: Ciênc. Agrár.

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Plant growth-promoting bacteria (PGPB) are known to establish positive relationships with plants. They act in favoring plant nutrition, production of phytohormones, control of pathogens and enhancement of stress tolerance. Thus, this study aimed to isolate bacteria from soil, rhizosphere, and root endosphere from sugarcane cultivated in the Southeastern of Brazil, to prospect strains with potential for plant growth promotion. The samples were plated in Nutrient Agar medium, and the morphologically distinct colonies were isolated and analyzed about indoleacetic acid production, phosphate solubilization and the growth control of the phytopathogenic fungus Fusarium verticillioides. A total of 219 isolates were obtained, of which 86 from soil, 67 from rhizosphere and 66 from sugarcane root endosphere. The strains that presented more than one mechanism of plant growth promotion were identified by the sequencing of 16S gene. Most species belonged to the genus Bacillus, which has strains already used in various biological products for the control of diseases in agriculture. Some Bacillus species isolated in our study have never been isolated from sugarcane, and others have been studied for the first time as plant growth promoters. The isolated strains constitute an important microbial bank to be explored to compose innovative products for agriculture.

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Section: Introductionmentioning

confidence: 99%

Screening of plant growth-promoting bacteria isolated from sugarcane

Bezerra

Takita

Tosta

et al. 2022

Semina: Ciênc. Agrár.

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“…One such class of agents are lectins, which comprise a diverse family of carbohydrate-binding proteins that recognize and bind to glycans with sugar-specificity. Lectins are found in organismal proteomes [7,8], and secretomes [9,10] across all kingdoms of life, and have a variation of ligand selectivity that rivals' that of antibodies. In many cases mammalian cell-surface binding of lectins, and subsequent intracellular transport, can modulate a manifold of biologic pathways with remarkable potency [11].…”

Section: Introductionmentioning

confidence: 99%

Discovery of antitumor lectins from rainforest tree root transcriptomes

et al. 2020

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Glycans are multi-branched sugars that are displayed from lipids and proteins. Through their diverse polysaccharide structures they can potentiate a myriad of cellular signaling pathways involved in development, growth, immuno-communication and survival. Not surprisingly, disruption of glycan synthesis is fundamental to various human diseases; including cancer, where aberrant glycosylation drives malignancy. Here, we report the discovery of a novel mannose-binding lectin, ML6, which selectively recognizes and binds to these irregular tumor-specific glycans to elicit potent and rapid cancer cell death. This lectin was engineered from gene models identified in a tropical rainforest tree root transcriptome and is unusual in its six canonical mannose binding domains (QxDxNxVxY), each with a unique amino acid sequence. Remarkably, ML6 displays antitumor activity that is >10 5 times more potent than standard chemotherapeutics, while being almost completely inactive towards non-transformed, healthy cells. This activity, in combination with results from glycan binding studies, suggests ML6 differentiates healthy and malignant cells by exploiting divergent glycosylation pathways that yield naïve and incomplete cell surface glycans in tumors. Thus, ML6 and other high-valence lectins may serve as novel biochemical tools to elucidate the glycomic signature of different human tumors and aid in the rational design of carbohydratedirected therapies. Further, understanding how nature evolves proteins, like ML6, to combat the changing defenses of competing microorganisms may allow for fundamental advances in the way we approach combinatorial therapies to fight therapeutic resistance in cancer. OPEN ACCESS Citation: Lawanprasert A, Guinan CA, Langford EA, Hawkins CE, Sloand JN, Fescemyer HW, et al. (2020) Discovery of antitumor lectins from rainforest tree root transcriptomes. PLoS ONE 15 (2): e0229467. https://doi.org/10.

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“…And why are some molecules, from microbes and/or plants, harmful enough to destroy hectares of cultivars (Shieh et al, 1997;Weir et al, 2003;Oerke, 2006;Ejeta and Gressel, 2007;Joel et al, 2007)? With the advance of new technologies, such as proteomics and metabolomics, the ability to identify secreted molecules has revealed important clues about their possible functions (De-la-Peña and Loyola-Vargas, 2012;Badri et al, 2013;Martin et al, 2014;Neumann et al, 2014), but the ecological and environmental relevance of many of them remains unresolved. In this review, we will focus on how root exudates are key players involved in the selection of the microbial community during plant-microbe interaction and how this interaction affects the productivity of plants in the field.…”

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confidence: 99%

Biotic Interactions in the Rhizosphere: A Diverse Cooperative Enterprise for Plant Productivity

De‐la‐Peña

Loyola‐Vargas

2014

PLANT PHYSIOLOGY

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Microbes and plants have evolved biochemical mechanisms to communicate with each other. The molecules responsible for such communication are secreted during beneficial or harmful interactions. Hundreds of these molecules secreted into the rhizosphere have been identified, and their functions are being studied in order to understand the mechanisms of interaction and communication among the different members of the rhizosphere community. The importance of root and microbe secretion to the underground habitat in improving crop productivity is increasingly recognized, with the discovery and characterization of new secreting compounds found in the rhizosphere. Different omic approaches, such as genomics, transcriptomics, proteomics, and metabolomics, have expanded our understanding of the first signals between microbes and plants. In this review, we highlight the more recent discoveries related to molecules secreted into the rhizosphere and how they affect plant productivity, either negatively or positively. In addition, we include a survey of novel approaches to studying the rhizosphere and emerging opportunities to direct future studies.

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The Hidden Chemical Cross-Talk Between Roots and Microbes: A Proteomic Approach

Cited by 5 publications

References 168 publications

Screening of plant growth-promoting bacteria isolated from sugarcane

Screening of plant growth-promoting bacteria isolated from sugarcane

Discovery of antitumor lectins from rainforest tree root transcriptomes

Biotic Interactions in the Rhizosphere: A Diverse Cooperative Enterprise for Plant Productivity

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