The Heterologous Expression of the Iris lactea var. chinensis Type 2 Metallothionein IlMT2b Gene Enhances Copper Tolerance in Arabidopsis thaliana

Gu, Chao; Liu, Liangqin; Deng, Yanming; Zhu, Xudong; Huang, Shihua; Lu, Xiaoyan

doi:10.1007/s00128-014-1444-x

Cited by 53 publications

(18 citation statements)

References 29 publications

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“…To date, plant MTs have been widely characterized, exhibiting different tissue-specific expression patterns [8,14,49,50,51,52]. For example, AtMT1A and AtMT2B were predominantly expressed in roots and leaves, while AtMT2A and AtMT3 were highly expressed in roots and young leaves [14].…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Characterization and Analysis of Metallothionein Family Genes That Function in Metal Stress Tolerance in Brassica napus L.

Pan

Zhu

Wang

et al. 2018

IJMS

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Brassica plants exhibit both high biomass productivity and high rates of heavy metal absorption. Metallothionein (MT) proteins are low molecular weight, cysteine-rich, metal-binding proteins that play crucial roles in protecting plants from heavy metal toxicity. However, to date, MT proteins have not been systematically characterized in Brassica. In this study, we identified 60 MTs from Arabidopsis thaliana and five Brassica species. All the MT family genes from Brassica are closely related to Arabidopsis MTs, encoding putative proteins that share similar functions within the same clades. Genome mapping analysis revealed high levels of synteny throughout the genome due to whole genome duplication and segmental duplication events. We analyzed the expression levels of 16 Brassica napus MTs (BnaMTs) by RNA-sequencing and real-time RT-PCR (RT-qPCR) analysis in plants under As3+ stress. These genes exhibited different expression patterns in various tissues. Our results suggest that BnaMT3C plays a key role in the response to As3+ stress in B. napus. This study provides insight into the phylogeny, origin, and evolution of MT family members in Brassica, laying the foundation for further studies of the roles of MT proteins in these important crops.

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Section: Discussionmentioning

confidence: 99%

“…Therefore, plants have evolved a suite of mechanisms involving the chelation and sequestration of heavy metals by various amino acids, organic acids, phytochelatins (PCs) and metallothioneins (MTs) [3,4]. These compounds play crucial roles in protecting plants from heavy metal toxicity [5,6,7,8,9]. …”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Characterization and Analysis of Metallothionein Family Genes That Function in Metal Stress Tolerance in Brassica napus L.

Pan

Zhu

Wang

et al. 2018

IJMS

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“…The combination of low kinetic stability and high thermodynamic is the main features of metal-MT complexes, which bind the metals very firmly, while a part of the metal ions is easily exchanged for other proteins (Maret, 2000 ). Transgenic plants overexpressing MTs genes have been scored for enhanced metal tolerance and they demonstrate modified metal accumulation or distribution strategies (Gu et al, 2015 ; Liu et al, 2015a ; Tomas et al, 2015 ). In plants, vacuole is considered as the final destination of detoxification of HMs.…”

Section: Heavy Metals (Hms) Detoxificationmentioning

confidence: 99%

Responses of Plant Proteins to Heavy Metal Stress—A Review

et al. 2017

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Plants respond to environmental pollutants such as heavy metal(s) by triggering the expression of genes that encode proteins involved in stress response. Toxic metal ions profoundly affect the cellular protein homeostasis by interfering with the folding process and aggregation of nascent or non-native proteins leading to decreased cell viability. However, plants possess a range of ubiquitous cellular surveillance systems that enable them to efficiently detoxify heavy metals toward enhanced tolerance to metal stress. As proteins constitute the major workhorses of living cells, the chelation of metal ions in cytosol with phytochelatins and metallothioneins followed by compartmentalization of metals in the vacuoles as well as the repair of stress-damaged proteins or removal and degradation of proteins that fail to achieve their native conformations are critical for plant tolerance to heavy metal stress. In this review, we provide a broad overview of recent advances in cellular protein research with regards to heavy metal tolerance in plants. We also discuss how plants maintain functional and healthy proteomes for survival under such capricious surroundings.

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“…Phytochelatins (PCs) and metallothioneins (MTs) are the most well-characterized heavy metal-binding ligands in plants. Numerous reports have shown that MTs play crucial roles in maintaining metal homeostasis and protect against heavy metal toxicity through intracellular sequestration [1][2][3][4][5].…”

mentioning

confidence: 99%

Characterization analysis and heavy metal‐binding properties of CsMTL3 in Escherichia coli

Liu

et al. 2018

FEBS Open Bio

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Members of the metallothionein ( MT ) superfamily are involved in coordinating transition metal ions. In plants, MT family members are characterized by their arrangement of Cys residues. In this study, one member of the MT superfamily, Cs MTL 3 , was characterized from a complementary DNA ( cDNA ) library from young cucumber fruit; Cs MTL 3 is predicted to encode a 64 amino acid protein with a predicted molecular mass of 6.751 kDa. Phylogenetic analysis identified it as a type 3 family member as the arrangement of N‐terminal Cys residues was different from that of MT‐like 2. Heterologous expression of Cs MTL 3 in Escherichia coli improved their heavy metal tolerance, particularly to Cd 2+ and Cu 2+ , and led to increased uptake of Cd 2+ and Cu 2+ ; increased uptake was also observed for cells expressing Arabidopsis thaliana metallothionein 3 (At MT 3) and phytochelatin‐like ( PCL ), with greatest uptake in PCL ‐expressing cells. These findings demonstrate that Cs MTL 3 can improve metal tolerance, especially for Cd 2+ ions, when heterologously expressed in E. coli , and suggest that the composition and arrangement of N‐terminal Cys residues are associated with binding capacity and preference for different metal ions.

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The Heterologous Expression of the Iris lactea var. chinensis Type 2 Metallothionein IlMT2b Gene Enhances Copper Tolerance in Arabidopsis thaliana

Cited by 53 publications

References 29 publications

Genome-Wide Characterization and Analysis of Metallothionein Family Genes That Function in Metal Stress Tolerance in Brassica napus L.

Genome-Wide Characterization and Analysis of Metallothionein Family Genes That Function in Metal Stress Tolerance in Brassica napus L.

Responses of Plant Proteins to Heavy Metal Stress—A Review

Characterization analysis and heavy metal‐binding properties of CsMTL3 in Escherichia coli

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