The Heterologous Expression of the Chrysanthemum R2R3-MYB Transcription Factor CmMYB1 Alters Lignin Composition and Represses Flavonoid Synthesis in Arabidopsis thaliana

Zhu, Li; Shan, Hong; Chen, Sumei; Jiang, Jiafu; Gu, Chao; Zhou, Guoqin; Chen, Yu; Song, Aiping; Chen, Fadi

doi:10.1371/journal.pone.0065680

Cited by 65 publications

(42 citation statements)

References 54 publications

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“…The expression of GmMYB100 was inhibited under abiotic stresses, such as MeJA, SA and sucrose, which can stimulate soybean flavonoid production. The deduced protein sequence of GmMYB100 was highly homologous to other plant MYB proteins, such as GbMYBF2 (Xu et al 2014), CmMYB1 (Zhu et al 2013), FaMYB1 (Paolocci et al 2011), EgMYB1 (Legay et al 2010, ZmMYB31 (Fornalé et al 2010), ZmMYB42 (Sonbol et al 2009), and GhMYB6, AtMYB308 and AtMYB4 (Jin et al 2000). In addition, protein motif search revealed a conserved repression domain ''pdLNLD/ELxiG/S'' (ERF-associated Amphiphilic Repression motif, EAR motif) in GmMYB100.…”

Section: Discussionmentioning

confidence: 93%

“…These results suggest that GmMYB100 is a possible repressor in the flavonoid biosynthesis. R2R3 MYB transcription factor CmMYB1 had the highest expression level in stems and affected the lignin composition in Chrysanthemum (Zhu et al 2013). The expression of GmMYB100 appeared to be distinct among different soybean tissues and its high level was found in immature embryo.…”

Section: Discussionmentioning

confidence: 99%

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The soybean R2R3 MYB transcription factor GmMYB100 negatively regulates plant flavonoid biosynthesis

et al. 2015

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Soybean flavonoids, a group of important signaling molecules in plant-environment interaction, ubiquitously exist in soybean and are tightly regulated by many genes. Here we reported that GmMYB100, a gene encoding a R2R3 MYB transcription factor, is involved in soybean flavonoid biosynthesis. GmMYB100 is mainly expressed in flowers, leaves and immature embryo, and its level is decreased after pod ripening. Subcellular localization assay indicates that GmMYB100 is a nuclear protein. GmMYB100 has transactivation ability revealed by a yeast functional assay; whereas bioinformatic analysis suggests that GmMYB100 has a negative function in flavonoid biosynthesis. GmMYB100-overexpression represses the transcript levels of flavonoid-related genes in transgenic soybean hairy roots and Arabidopsis, and inhibits isoflavonoid (soybean) and flavonol (Arabidopsis) production in transgenic plants. Furthermore, the transcript levels of six flavonoid-related genes and flavonoid (isoflavonoid and flavone aglycones) accumulation are elevated in the GmMYB100-RNAi transgenic hairy roots. We also demonstrate that GmMYB100 protein depresses the promoter activities of soybean chalcone synthase and chalcone isomerase. These findings indicate that GmMYB100 is a negative regulator in soybean flavonoid biosynthesis pathway.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

The soybean R2R3 MYB transcription factor GmMYB100 negatively regulates plant flavonoid biosynthesis

et al. 2015

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“…Two chrysanthemum MYB genes have been isolated so far. One of these, CmMYB1 , when constitutively expressed in A. thaliana , has a suppressive effect on tissue lignin content and flavonoid synthesis [14], while the other ( CmMYB2 ) not only enhances drought and salinity tolerance, but also increases the plant’s sensitivity to abscisic acid and defers flowering [15]. Here, the R2R3-MYB member CmMYB19 was shown to be inducible by aphid feeding and appears to contribute to host tolerance against aphid feeding.…”

Section: Discussionmentioning

confidence: 99%

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

Wang

Sheng

Zhang

et al. 2017

IJMS

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Abstract:The gene encoding the MYB (v-myb avian myeloblastosis vira l oncogene homolog) transcription factor CmMYB19 was isolated from chrysanthemum. It encodes a 200 amino acid protein and belongs to the R2R3-MYB subfamily. CmMYB19 was not transcriptionally activated in yeast, while a transient expression experiment conducted in onion epidermal cells suggested that the CmMYB19 product localized to the nucleus. CmMYB19 transcription was induced by aphid (Macrosiphoniella sanborni) infestation, and the abundance of transcript was higher in the leaf and stem than in the root. The over-expression of CmMYB19 restricted the multiplication of the aphids. A comparison of transcript abundance of the major genes involved in lignin synthesis showed that CmPAL1 (phenylalanine ammonia lyase 1), CmC4H (cinnamate4 hydroxylase), Cm4CL1 (4-hydroxy cinnamoyl CoA ligase 1), CmHCT (hydroxycinnamoyl CoA-shikimate/quinate hydroxycinnamoyl transferase), CmC3H1 (coumarate3 hydroxylase1), CmCCoAOMT1 (caffeoyl CoA O-methyltransferase 1) and CmCCR1 (cinnamyl CoA reductase1) were all upregulated, in agreement with an increase in lignin content in CmMYB19 over-expressing plants. Collectively, the over-expression of CmMYB19 restricted the multiplication of the aphids on the host, mediated by an enhanced accumulation of lignin.

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“…Chrysanthemum spp. MYB1, the repressor of lignin biosynthesis, also downregulates the flavonoid pathway (Zhu et al, 2013).…”

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confidence: 99%

The MYB182 Protein Down-Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Poplar by Repressing Both Structural and Regulatory Flavonoid Genes

2015

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Trees in the genus Populus (poplar) contain phenolic secondary metabolites including the proanthocyanidins (PAs), which help to adapt these widespread trees to diverse environments. The transcriptional activation of PA biosynthesis in response to herbivory and ultraviolet light stress has been documented in poplar leaves, and a regulator of this process, the R2R3-MYB transcription factor MYB134, has been identified. MYB134-overexpressing transgenic plants show a strong high-PA phenotype. Analysis of these transgenic plants suggested the involvement of additional MYB transcription factors, including repressor-like MYB factors. Here, MYB182, a subgroup 4 MYB factor, was found to act as a negative regulator of the flavonoid pathway. Overexpression of MYB182 in hairy root culture and whole poplar plants led to reduced PA and anthocyanin levels as well as a reduction in the expression of key flavonoid genes. Similarly, a reduced accumulation of transcripts of a MYB PA activator and a basic helix-loop-helix cofactor was observed in MYB182-overexpressing hairy roots. Transient promoter activation assays in poplar cell culture demonstrated that MYB182 can disrupt transcriptional activation by MYB134 and that the basic helix-loop-helix-binding motif of MYB182 was essential for repression. Microarray analysis of transgenic plants demonstrated that down-regulated targets of MYB182 also include shikimate pathway genes. This work shows that MYB182 plays an important role in the fine-tuning of MYB134-mediated flavonoid metabolism.

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The Heterologous Expression of the Chrysanthemum R2R3-MYB Transcription Factor CmMYB1 Alters Lignin Composition and Represses Flavonoid Synthesis in Arabidopsis thaliana

Cited by 65 publications

References 54 publications

The soybean R2R3 MYB transcription factor GmMYB100 negatively regulates plant flavonoid biosynthesis

The soybean R2R3 MYB transcription factor GmMYB100 negatively regulates plant flavonoid biosynthesis

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

The MYB182 Protein Down-Regulates Proanthocyanidin and Anthocyanin Biosynthesis in Poplar by Repressing Both Structural and Regulatory Flavonoid Genes

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