The heterogeneity of protein kinase C in various rat tissues

Kosaka, Yoshiyuki; Ogita, Kouji; Ase, Katsuhiko; Nomura, Hiroyuki; Kikkawa, Ushio; Nishizuka, Yasutomi

doi:10.1016/s0006-291x(88)80461-3

Cited by 203 publications

(95 citation statements)

References 22 publications

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“…CKmCIB-a, a monoclonal antibody (MAb) reactive to conventional PKC subtypes (18). has been shown previously to react with sperm PKC (14-16) and was kindly provided by Dr. Y. Nishizuka (Kobe, Japan).…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural localization of protein kinase C in human sperm.

Kalina¹,

Socher²,

Rotem³

et al. 1995

J Histochem Cytochem.

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We localized protein kinase C (PKC) in human sperm cells at the ultrasrructural level by the immunogold technique. The sperm head PKC was localized in the acrosome, equatorial segment, and post-acrosomal region. In the flagellum, PKC was associated with the segmented column of the neck and was distributed along the mid, principal, and end pieces. Immunoreactive sites were observed in patches along the axoneme and outer dense fibers and were evenly distributed between these regions. Pre-absorption of the antibody used with rat brain PKC (a and P) eliminated gold

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Section: Methodsmentioning

confidence: 99%

Ultrastructural localization of protein kinase C in human sperm.

Kalina¹,

Socher²,

Rotem³

et al. 1995

J Histochem Cytochem.

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“…Immunoblot analysis lmmunoblot analysis was carried out as described [21]. The PKC fractions of hydroxyapatite column chromatography were subjected to SDS-polyacrylamide gel elcctrophoresis, and transferred to a nitrocellulose filter.…”

Section: Assay Of Pkcmentioning

confidence: 99%

Protein kinase C of a human megakaryoblastic leukemic cell line (MEG‐01) Analysis of subspecies and activation by diacylglycerol and free fatty acids

et al. 1991

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Protein kinase C (PKC) from a human mcgakaryoblastic leukemic cell line (MEG-01) was resolved into two fractions by hydroxyapatitc column chromatography, which arc indistinguishable. from the brain type II (j?I//?II) and type III (u) subspecies, by biochemical and immunoblot analysis. In the presence of both phosphatidylserine and diacylglycerol, several free unsaturated fatty acids (FFA's), such as arachidonic, oleic, linoleic and linolenic acids, further enhanced the enzyme activation, and allowed the enzyme to exhibit almost full activity at nearly basal levels of Cat+ conccntration. The concentration of unsaturated FFA's giving rise to the maximum enzyme activation was around 2 x lO-s M. Palmitic and stearic acids were inactive. The result implies that, in addition to diacylglycerol, the receptor-mediated release of unsaturated FFA's from membrane phospholipids may also take part in the activation of PKC.

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“…Detailed procedures and properties of these antisera were described [13,14]. Sections from rats injected with BrdU were examined for DNA replication with a cell proliferation kit (Amersham, UK).…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Heterogeneous activation of protein kinase C during rat liver regeneration induced by carbon tetrachloride administration

et al. 1989

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During rat liver regeneration induced by carbon tetrachloride administration, the protein kinase C a subspecies was activated in a heterogeneous fashion, a higher number of hepatocytes expressing the protein kinase C a subspecies being detected in the pericentral zone than in the periportal zone. This zonal heterogeneity became maximal at 24 h after the treatment. The distribution of hepatocytes expressing the protein kinase C a subspecies was roughly coincident with that of hepatocytes exhibiting DNA synthesis. These results suggest that protein kinase C may play a crucial role in liver regeneration.

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The heterogeneity of protein kinase C in various rat tissues

Cited by 203 publications

References 22 publications

Ultrastructural localization of protein kinase C in human sperm.

Ultrastructural localization of protein kinase C in human sperm.

Protein kinase C of a human megakaryoblastic leukemic cell line (MEG‐01) Analysis of subspecies and activation by diacylglycerol and free fatty acids

Heterogeneous activation of protein kinase C during rat liver regeneration induced by carbon tetrachloride administration

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