The het-c heterokaryon incompatibility gene in Aspergillus niger

Diepeningen, A.D. van; Pál, Károly; Lee, Theo A. J. van der; Hoekstra, Rolf F.; Debets, A.J.M.

doi:10.1016/j.mycres.2008.10.003

Cited by 15 publications

(21 citation statements)

References 38 publications

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“…Moreover, two of the consensus phosphorylation sites (FXXT/S) identified in Aspergillus nidulans two-hybrid interactor with NIMA C (TINC) (Davies et al 2004) were conserved in the T. melanosporum paralogs. These functional and structural features are also shared with orthologous proteins characterized in other ascomycetes (Saupe et al 1996;Davies et al 2004;Van Diepeningen et al 2008).…”

Section: Het-cmentioning

confidence: 74%

“…However, allelic interactions between alternative het-c alleles are not sufficient to confer nonself recognition and HI in N. crassa. The lack of linkage between members of this HI system was instead found in Aspergillus niger where the loci of het-c and the closest hit of the pin-c gene are located in different contigs (Van Diepeningen et al 2008). No evidence for pin-c homologue closely linked to tmelhetC135 was detected in the T. melanosporum genome but putative genes encoding an alpha-1,3-glucan synthase and a RNA binding protein not related with HI are located w10 kb upstream and w3 kb downstream, respectively (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, point mutations in the HET domain of N. crassa pin-c and P. anserina het-e genes suppress HI reactions (Kaneko et al 2006;. Molecular characterization of het-like genes has also been performed in other ascomycetes, such as Botrytis cinerea (Fournier et al 2003), Fusarium proliferatum (Ker enyi et al 2006, and Aspergillus niger (Van Diepeningen et al 2008), but the homologues examined did not exhibit endogenous HI characteristics in these species.…”

Section: Introductionmentioning

confidence: 99%

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Self/nonself recognition in Tuber melanosporum is not mediated by a heterokaryon incompatibility system

et al. 2012

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Section: Het-cmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Self/nonself recognition in Tuber melanosporum is not mediated by a heterokaryon incompatibility system

et al. 2012

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“…As a rule, however, when two fusing hyphae are not compatible, they recognize each other as nonself, which triggers programmed cell death (PCD). In aspergilli, the genes involved in activation of PCD reactions are currently being characterised and regarded as a promising approach to combat invasive aspergillosis [23][24][25][26]. Genetic incompatibility is mostly followed by death of the hyphal fusion cell, the so-called heterokaryon, and often of surrounding cells.…”

Section: Intracellular Transmissionmentioning

confidence: 99%

Mycoviruses: future therapeutic agents of invasive fungal infections in humans?

Sande

Lo-Ten-Foe

Belkum

et al. 2010

Eur J Clin Microbiol Infect Dis

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“…Recent efforts to identify fungal vic genes based on sequence homology with N. crassa and P. anserina vic genes have not been fruitful (Fournier et al 2003;Kerenyi et al 2006;Pal et al 2007;Van Diepeningen et al 2008;Iotti et al 2012;Van Der Nest et al 2014). Using a strategy based on polymorphism-guided comparative genomics and on information about vic genetic markers, we recently identified seven incompatibility genes associated with four of the six C. parasitica vic loci (Choi et al 2012).…”

mentioning

confidence: 99%

Vegetative Incompatibility Loci with Dedicated Roles in Allorecognition Restrict Mycovirus Transmission in Chestnut Blight Fungus

et al. 2014

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Vegetative incompatibility (vic), a form of nonself allorecognition, operates widely in filamentous fungi and restricts transmission of virulence-attenuating hypoviruses in the chestnut blight fungus Cryphonectria parasitica. We report here the use of a polymorphism-based comparative genomics approach to complete the molecular identification of the genetically defined C. parasitica vic loci with the identification of vic1 and vic3. The vic1 locus in the C. parasitica reference strain EP155 consists of a polymorphic HET-domain-containing 771-aa ORF designated vic1a-2, which shares 91% identity with the corresponding vic1a-1 allele, and a small (172 aa) idiomorphic DUF1909-domain-containing ORF designated vic1b-2 that is absent at the vic1-1 locus. Gene disruption of either vic1a-2 or vic1b-2 in strain EP155 eliminated restrictions on virus transmission when paired with a vic1 heteroallelic strain; however, only disruption of vic1a-2 abolished the incompatible programmed cell death (PCD) reaction. The vic3 locus of strain EP155 contains two polymorphic ORFs of 599 aa (vic3a-1) and 102 aa (vic3b-1) that shared 46 and 85% aa identity with the corresponding vic3a-2 and vic3b-2 alleles, respectively. Disruption of either vic3a-1 or vic3b-1 resulted in increased virus transmission. However, elimination of PCD required disruption of both vic3a and vic3b. Additional allelic heterogeneity included a sequence inversion and a 8.5-kb insertion containing a LTR retrotransposon sequence and an adjacent HET-domain gene at the vic1 locus and a 7.7-kb sequence deletion associated with a nonfunctional, pseudo vic locus. Combined gene disruption studies formally confirmed restriction of mycovirus transmission by five C. parasitica vic loci and suggested dedicated roles in allorecognition. The relevance of these results to the acquisition and maintenance of vic genes and the potential for manipulation of vic alleles for enhanced mycovirus transmission are discussed.A LLORECOGNITION genetic systems, which provide the ability to distinguish self from nonself, play important functional roles in microbial and multicellular organisms. These systems range from restriction endonucleases in bacteria (Meselson and Yuan 1968) to somatic histocompatibility in protocordates (De Tomaso et al. 2005), self-infertility in plants (Nasrallah 2005), and innate immunity in vertebrates (Medzhitov and Janeway 2002) (reviewed by Aanen et al. 2008;Nydam and De Tomaso 2011;Rosengarten and Nicotra 2011). Allorecognition operates widely in filamentous fungi in both the sexual and the vegetative growth phases (Saupe 2000). The role of the mating-type locus in controlling sexual recognition and promoting outbreeding in yeast and filamentous fungi is well understood (reviewed by Coppin et al. 1997). It is also known that somatic or vegetative fusion of fungal cells (termed "anastomosis") occurs at a high frequency within and between individuals promoting network formation (Rayner 1996) and facilitating foraging, the pooling of resources (Rayner 1996), ...

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The het-c heterokaryon incompatibility gene in Aspergillus niger

Cited by 15 publications

References 38 publications

Self/nonself recognition in Tuber melanosporum is not mediated by a heterokaryon incompatibility system

Self/nonself recognition in Tuber melanosporum is not mediated by a heterokaryon incompatibility system

Mycoviruses: future therapeutic agents of invasive fungal infections in humans?

Vegetative Incompatibility Loci with Dedicated Roles in Allorecognition Restrict Mycovirus Transmission in Chestnut Blight Fungus

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