The herbal decoction modified Danggui Buxue Tang attenuates immune-mediated bone marrow failure by regulating the differentiation of T lymphocytes in an immune-induced aplastic anemia mouse model

Abstract

Angelicae Sinensis, Radix Astragali and Rhizoma Coptidis are all herbs of modified Danggui Buxue Tang (DGBX) and are extensively applied herbs in traditional Chinese medicine for the treatment of anemia and inflammation. In this study, immune-induced AA mice were used as an animal model, and the immunosuppressive agent, Ciclosporin A (CsA), was used as a positive control. Multiple pro-inflammatory cytokines were examined by bead-based multiplex flow cytometry. The T-cell subsets were assessed using a fluorescence-activated cell sorter (FACS). Western blot analysis was used to estimate the protein expression levels of specific transcription factors for T helper cells (Th1, Th2 and Th17) and key molecules of the Janus-activated kinase (Jak)/signal transducer and activator of transcription (Stat3) signaling pathway. DGBX treatment could significantly increase the production of whole blood cells in peripheral blood (PB); inhibit the expansion of Th1 and Th17 cells; increase the differentiation of Th2 and Tregs cells; regulate the expression levels of T-bet, GATA-3, RORγ and proinflammatory cytokines; and decrease the expression levels of key molecules in the Jak/Stat signaling pathway. These results indicate that DGBX can regulate the differentiation of T lymphocytes, resulting in immunosuppressive and hematogenic functions on AA mice. DGBX might be a good candidate for inclusion in a randomized study for AA with more data on the possible side effects and doses used in humans. Ultimately, it may be used for applications of traditional medicine against AA in modern complementary and alternative immunosuppressive therapeutics.

Blood-Enriching Effects and Immune-Regulation Mechanism of Steam-Processed Polygonatum Sibiricum Polysaccharide in Blood Deficiency Syndrome Mice

“…In this pathway, Jak1, Stat1, GATA1and EPOR were the major targets to treat BDS ( 7 ). Danggui Buxue Tang could significantly attenuated the levels of key targets (Jak1, Stat1 and Stat3) of the Jak/Stat signaling pathway in immune-induced aplastic anemia mice ( 53 ). Jujube polysaccharides have been showed the improvement on chronic kidney disease-associated anemia by alternating the serum EPO level, kidney EPO mRNA and protein expression ( 54 ).…”

Antifungal Potential of Some Herb Decoctions and Essential Oils on Candida Species

“…In TCM, herbal decoctions are specific combinations of different herbs that are used as formulas according to the patient’s symptoms and characteristics in order to treat various diseases, including infections [ 25 ]. EOs are natural products produced by aromatic plants and are rich in terpenes and terpenoids.…”

Coptidis alkaloids extracted from Coptis chinensis Franch attenuate IFN-γ-induced destruction of bone marrow cells

“…The constituents of Coptis chinensis were analyzed by HPLC-ESI/MS n . The specific measurement procedures referred to our previous works methods as previously described [12][13][14]. Peak View Software 2.2 was used to analyze the data, including retention time, accurate mass and MS/MS spectrum comparison.…”