The hemoglobins of the bullfrog, Rana catesbeiana. The cDNA-derived amino acid sequences of the alpha chains of adult hemoglobins B and C: their roles in deoxygenation-induced aggregation.

Smith, David J.; Zhu, Hao; Kolatkar, Prasanna R.; Tam, Lei-Ting; Baldwin, Thomas O.; Roe, Bruce A.; Broyles, Robert W.; Riggs, Austen

doi:10.1016/s0021-9258(19)74204-5

Cited by 11 publications

(1 citation statement)

References 52 publications

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“…The combination of two alpha chains and two non‐alpha chains produces a mature hemoglobin molecule (de Araújo, Ribeiro, & de Araújo, ). Moreover, the changes in these gene expressions reflect hemoglobin molecule changes in tadpoles (Smith et al, ). In the present study, the timing of increasing expression of members of the hemoglobin gene family was harmonized with formation of the lung, around NF stage 57 (Nieuwkoop & Faber, ), indicating that the increases in these gene expressions might reflect preparation of pulmonary respiration.…”

Section: Discussionmentioning

confidence: 99%

A microarray‐based comparative analysis of gene expression profiles in thyroid glands in amphibian metamorphosis: differences in effects between chemical exposure and food restriction

Ose

Yamada

Ôhara

et al. 2019

J of Applied Toxicology

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Tadpoles during metamorphosis are sensitive to chemical exposure as shown in the amphibian metamorphosis assay, which is a method to detect effects of chemicals on the functions of hypothalamus‐pituitary‐thyroid axis. The present study reports existence of different modes of action between pyriproxyfen (PYR) and 6‐propyl‐2‐thiouracil (PTU) under different feeding conditions based on gene expression profiles (transcriptomics) in the thyroid glands of tadpoles of the African clawed frog, Xenopus laevis. PTU and PYR were exposed to the tadpoles during metamorphosis under normal (fed groups, both of PTU and PRY) and restricted feeding (fasted groups, PTU only) conditions; and effects were compared to control groups. Delayed development based on decreased Nieuwkoop and Faber developmental stage number without any histopathological changes was observed in the control of restricted feeding (control‐fasted) group, and the PYR group with reduced food consumption. Clear developmental retardation with typical thyroid histopathological changes was observed in the PTU groups. To find clusters of all samples based on their similarity of expression patterns, hierarchical clustering analysis using selected gene probes was conducted. It revealed gene profiles from samples of the PYR group were quite similar to those of the control‐fasted group, followed by the control group with normal feeding (control‐fed). The results suggest that key events in the thyroid glands of tadpoles induced by PYR should be quite similar to those of control‐fasted, and quite different from those of the PTU groups. Our findings demonstrated the usefulness of transcriptomics, which enabled recognition of the different modes of actions.

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Section: Discussionmentioning

confidence: 99%

A microarray‐based comparative analysis of gene expression profiles in thyroid glands in amphibian metamorphosis: differences in effects between chemical exposure and food restriction

Ose

Yamada

Ôhara

et al. 2019

J of Applied Toxicology

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Partial characterization of a novel amphibian hemoglobin as a model for graduate student investigation on peptide chemistry, mass spectrometry, and atomic force microscopy

Bemquerer

Macêdo

Ribeiro

et al. 2011

Biochem Molecular Bio Educ

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Graduate students in chemistry, and in biological and biomedical fields must learn the fundamentals and practices of peptide and protein chemistry as early as possible. A project-oriented approach was conducted by first-year M.Sc and Ph.D students in biological sciences. A blind glass slide containing a cellular smear and an aqueous cellular extract were offered to the students. Qualitative and quantitative cell morphological parameters were analyzed by atomic force microscopy. The fractionation of the aqueous extract was conducted by reversed-phase chromatography followed by analysis of the isolated and partially purified proteins and peptides by mass spectrometry (MS). The proteins were treated by peptidases and the obtained peptide fragments were sequenced by de novo MS/MS, together with peptides already present in the extract. The most abundant protein fractions were identified as the alpha and beta chains of hemoglobin from an amphibian of the Leptodactylus genera. Two of the peptides sequenced by the students were synthesized by the solid-phase methodology, one of those being obtained by the splitand-pool library synthesis method. Thus, the students were able to learn some advanced principles and practices of protein chemistry and bionanotechnology in a 6-weeks project-oriented approach.

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Vertebrate Hemoglobins

Ingermann¹

1997

Comprehensive Physiology

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The hemoglobins of the bullfrog, Rana catesbeiana. The cDNA-derived amino acid sequences of the alpha chains of adult hemoglobins B and C: their roles in deoxygenation-induced aggregation.

Cited by 11 publications

References 52 publications

A microarray‐based comparative analysis of gene expression profiles in thyroid glands in amphibian metamorphosis: differences in effects between chemical exposure and food restriction

A microarray‐based comparative analysis of gene expression profiles in thyroid glands in amphibian metamorphosis: differences in effects between chemical exposure and food restriction

Partial characterization of a novel amphibian hemoglobin as a model for graduate student investigation on peptide chemistry, mass spectrometry, and atomic force microscopy

Vertebrate Hemoglobins

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