The Heat Tolerance of Dry Colonies of a Terrestrial Cyanobacterium, &lt;i&gt;Nostoc&lt;/i&gt; sp. HK-01

Kimura, Shunta; Tomita‐Yokotani, Kaori; Igarashi, Yuichi; Sato, Seigo; Katoh, Hiroshi; Abe, Tomoko; Sonoike, Kintake; Ohmori, Masayuki

doi:10.2187/bss.29.12

Cited by 10 publications

(14 citation statements)

References 37 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For all experiments in this study, we used a pure culture of the terrestrial cyanobacterium Nostoc sp. HK-01, stocked at the Ohmori Laboratory (present affiliation; Chuo University) [32] [34].…”

Section: Cyanobacterium and Chemicalsmentioning

confidence: 99%

See 1 more Smart Citation

Compatible Solutes in the Akinetes of the Terrestrial Cyanobacterium Nostoc sp. HK-01 Contribute to Its Heat Tolerance

Kimura¹,

Ong²,

Ichikawa³

et al. 2017

AJPS

Self Cite

View full text Add to dashboard Cite

The detailed mechanisms that facilitate the heat tolerance of terrestrial cyanobacteria have not been completely elucidated, although several reports have revealed aspects of the heat tolerance mechanisms of several other organisms. The dormant cells, called akinetes, of the terrestrial cyanobacterium Nostoc sp. HK-01 can revive after dry heat exposure at 100˚C for more than 10 h. We investigated the compatible solutes that protect the biomolecules in Nostoc sp. HK-01 akinetes using colonies containing various proportions of akinetes. We extracted the intracellular substances from each colony with 80% ethanol, which we purified with a series of analytical columns and analyzed by highperformance liquid chromatography and liquid chromatography-electrospray ionization-mass spectrometry. The compatible solutes were screened for their ability to prevent protein aggregation upon heating using the model enzyme lactate dehydrogenase. We detected an accumulation of glucosylglycerol, betaine, and glycine in akinetes. In addition, we confirmed that betaine, glycine, sucrose, and trehalose contributed to the prevention of the protein aggregation. The levels of sucrose and glycine in the colonies were approximately 1000× higher than those of glucosylglycerol, betaine, or trehalose. Our results indicated that sucrose and glycine are the main compatible solutes in the hydrophilic fractions of the cell extracts of Nostoc sp. HK-01 akinetes.

show abstract

Section: Cyanobacterium and Chemicalsmentioning

confidence: 99%

“…We prepared dried colonies as described previously [32]. Each group of cells was incubated in the liquid medium BG-11 [71], at 26˚C with light exposure at 74.…”

Section: Incubation and Preparation Of Coloniesmentioning

confidence: 99%

Compatible Solutes in the Akinetes of the Terrestrial Cyanobacterium Nostoc sp. HK-01 Contribute to Its Heat Tolerance

Kimura¹,

Ong²,

Ichikawa³

et al. 2017

AJPS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The akinetes on MRS exhibited the ability to both germinate and grow (Fig 2-C). Kimura et al (2015) reported that there are usually ca. 10 %-30 % akinetes of all the cells in Nostoc sp.…”

Section: Resultsmentioning

confidence: 99%

“…The cyanobacterium, Nostoc sp. HK-01, has a high tolerance for several environmental conditions, including vacuum, ultra violet light, gamma rays, heavy ion beam (He) radiation and dry heat (Arai et al, 2008;Tomita-Yokotani et al, 2013;Kimura et al, 2014Kimura et al, , 2015. These results support the possibility of human habitation on Mars, based on utilization of the cyanobacteria as food.…”

Section: Introductionmentioning

confidence: 81%

Evaluation of a Cyanobacterium, Nostoc sp. HK-01, as Food Material for Space Agriculture on Mars

Kimura

Sato

et al. 2015

Biol. Sci. Space

Self Cite

View full text Add to dashboard Cite

A terrestrial cyanobacterium, Nostoc sp. HK-01, is a candidate material for introduction to extraterrestrial environments, such as Mars, given its high tolerance to drought and other extraterrestrial environments. Here, we evaluated Nostoc sp. HK-01 as food for future habitation of Mars. We found that wet colonies of Nostoc sp. HK-01 after initiation survived for over 8 years on Martian regolith simulant (MRS), although they did not survive for over 105 days on medium without MRS. Total protein per 100 g of the dried colony of Nostoc sp. HK-01 was approximately 50 g. Increases in biomass proliferation in liquid or on agar medium were determined in six strains of terrestrial cyanobacteria, including Nostoc sp. HK-01, N. commune HK-02, N. commune YK-04, N. punctiforme ATCC 29133, Brasilonema sp. HK-05 and Calothrix sp. HK-06. Nostoc sp. HK-01 grew better compared to the other five strains under both conditions. Following the incubation of Nostoc sp. HK-01, the medium exhibited radical-scavenging activity. Sugar content in the medium after a 1-day incubation of Nostoc sp. HK-01 was one-sixth of its dry weight at initiation. These results indicate that, after liquid incubation, the remaining cells may be used as recyclable materials on Mars. ©2015 Jpn. Soc. Biol. Sci.

show abstract

“…In addition to Deinococcus spp., survivability of the other two microbes, namely dried filaments of Nostoc sp. KH-01, which show high resistance against heat (Kimura et al 2015), and the spores of Schizosaccharomyces pombe JY3, will be also investigated.…”

Section: B1 Survival I-iii Experimentsmentioning

confidence: 99%

Space as a Tool for Astrobiology: Review and Recommendations for Experimentations in Earth Orbit and Beyond

et al. 2017

View full text Add to dashboard Cite

The space environment is regularly used for experiments addressing astrobiology research goals. The specific conditions prevailing in Earth orbit and beyond, notably the radiative environment (photons and energetic particles) and the possibility to conduct long-duration measurements, have been the main motivations for developing experimental concepts to expose chemical or biological samples to outer space, or to use the reentry of a spacecraft on Earth to simulate the fall of a meteorite. This paper represents an overview of past and current research in astrobiology conducted in Earth orbit and beyond, with a special focus on ESA missions such as Biopan, STONE (on Russian FOTON capsules) and EXPOSE facilities (outside the International Space Station). The future of exposure platforms is discussed, notably how they can be improved for better science return, and how to incorporate the use of small satellites such as those built in cubesat format.

show abstract

The Heat Tolerance of Dry Colonies of a Terrestrial Cyanobacterium, <i>Nostoc</i> sp. HK-01

Abstract: The heat tolerance of dry colonies of a terrestrial cyanobacterium, Nostoc sp. HK-01 by exposure to temperatures at 100 ˚C, the boiling point of liquid water, was examined.

Cited by 10 publications

References 37 publications

Compatible Solutes in the Akinetes of the Terrestrial Cyanobacterium <i>Nostoc</i> sp. HK-01 Contribute to Its Heat Tolerance

Compatible Solutes in the Akinetes of the Terrestrial Cyanobacterium <i>Nostoc</i> sp. HK-01 Contribute to Its Heat Tolerance

Evaluation of a Cyanobacterium, <i>Nostoc</i> sp. HK-01, as Food Material for Space Agriculture on Mars

Space as a Tool for Astrobiology: Review and Recommendations for Experimentations in Earth Orbit and Beyond

Contact Info

Product

Resources

About

The Heat Tolerance of Dry Colonies of a Terrestrial Cyanobacterium, <i>Nostoc</i> sp. HK-01

Abstract: The heat tolerance of dry colonies of a terrestrial cyanobacterium, Nostoc sp. HK-01 by exposure to temperatures at 100 ˚C, the boiling point of liquid water, was examined.

Cited by 10 publications

References 37 publications

Compatible Solutes in the Akinetes of the Terrestrial Cyanobacterium &lt;i&gt;Nostoc&lt;/i&gt; sp. HK-01 Contribute to Its Heat Tolerance

Compatible Solutes in the Akinetes of the Terrestrial Cyanobacterium &lt;i&gt;Nostoc&lt;/i&gt; sp. HK-01 Contribute to Its Heat Tolerance

Evaluation of a Cyanobacterium, <i>Nostoc</i> sp. HK-01, as Food Material for Space Agriculture on Mars

Space as a Tool for Astrobiology: Review and Recommendations for Experimentations in Earth Orbit and Beyond

Contact Info

Product

Resources

About

Compatible Solutes in the Akinetes of the Terrestrial Cyanobacterium <i>Nostoc</i> sp. HK-01 Contribute to Its Heat Tolerance

Compatible Solutes in the Akinetes of the Terrestrial Cyanobacterium <i>Nostoc</i> sp. HK-01 Contribute to Its Heat Tolerance