The heat is on: room temperature affects laboratory equipment–an observational study

Butler, Julia M.; Johnson, Jane E.; Boone, William R.

doi:10.1007/s10815-013-0064-4

Cited by 19 publications

(8 citation statements)

References 15 publications

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“…However, even at the enzyme's optimum temperature (49 ± 2˚C), the free DNA (fDNA) recovery rate from this method is low at approximately 4.4% per hour; additionally, the enzyme's half-life is limiting at approximately 11.3 h [21][22]. At room temperature (�22˚C), the proteolytic reaction rate yields only 1.1% fDNA per hour [24]. DNA can also be recovered from formalin-fixed tissue with a 0.1 M NaOH (pH 12) buffer treatment at 120˚C for min [7,25].…”

Section: Fig 1 Schematic Of Formalin-induced Crosslink Formation Andmentioning

confidence: 99%

Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens

et al. 2020

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DNA from formalin-preserved tissue could unlock a vast repository of genetic information stored in museums worldwide. However, formaldehyde crosslinks proteins and DNA, and prevents ready amplification and DNA sequencing. Formaldehyde acylation also fragments the DNA. Treatment with proteinase K proteolyzes crosslinked proteins to rescue the DNA, though the process is quite slow. To reduce processing time and improve rescue efficiency, we applied the mechanical energy of a vortex fluidic device (VFD) to drive the catalytic activity of proteinase K and recover DNA from American lobster tissue (Homarus americanus) fixed in 3.7% formalin for >1-year. A scan of VFD rotational speeds identified the optimal rotational speed for recovery of PCR-amplifiable DNA and while 500+ base pairs were sequenced, shorter read lengths were more consistently obtained. This VFD-based method also effectively recovered DNA from formalin-preserved samples. The results provide a roadmap for exploring DNA from millions of historical and even extinct species.

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Section: Fig 1 Schematic Of Formalin-induced Crosslink Formation Andmentioning

confidence: 99%

Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens

et al. 2020

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“…The thirty temperatures, at each incubator position, were chosen during a time at which room temperature was most stable. Because room temperature affects equipment [6,7], room temperature also was recorded.…”

Section: Protocolmentioning

confidence: 99%

“…Scientists have conducted numerous studies to investigate the affect temperature has on embryo development and pregnancy rates [1][2][3][4]. Because temperature has a direct impact on embryo homeostasis [1], it is important to monitor and control temperatures in the ART laboratory [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Temperature variations within and between incubators—a prospective, observational study

Walker

Butler

Higdon

et al. 2013

J Assist Reprod Genet

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Purpose To determine if there is a temperature variation within and between incubators. Methods This prospective, experimental trial with external controls was performed at an Assisted Reproductive Technology laboratory in a tertiary-care, university hospital. Temperature values were taken at various locations within and between incubators. Results Even though they were both set to 37.0°C, the same make and model incubators had significantly different internal temperatures. Temperatures differed significantly among top, middle and bottom shelves and between fronts and backs of shelves. Conclusion(s) We found temperatures differed within and between our front-loading incubators. Thus, laboratory personnel should evaluate their incubators for temperature variations within and between incubators and, if temperatures differ significantly, develop a plan to deal with discrepancies.

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“…In practice, temperature stability of ambient (or room) temperature in a laboratory is vital during the in-vitro process to avoid detrimental conditions [39].The objective of the study is to evaluate the effect ambient temperature has on one heater chip. In laboratories, hot and cold spots occur depending on the use of either heating or cooling system, which can affect the ambient temperature.Generally, most laboratories' room temperatures fluctuate between 293 and 298 K [40].…”

Section: Ambient Temperature (Tα)mentioning

confidence: 99%

Section: Ambient Temperature (T α )mentioning

confidence: 99%

Numerical Investigation of Design and Operating Parameters of Thermal Gradient Continuous-Flow PCR Microreactor Using One Heater

Perwez¹,

Aziz²,

Ahmed³

et al. 2019

Processes

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To respond to the dire need for miniaturization and process simplification of continuous-flow PCR (CF-PCR) device, this paper represents design and operation guide of a novel metal alloy assisted hybrid microdevice (polydimethylsiloxane (PDMS) and glass) for CF-PCR employing one heater. In this research, the specific objectives are to determine whether one heater chip design will be flexible enough when the size of DNA base pair is varied and to investigate whether one heater CF-PCR device will be able to resolve the longstanding problem of thermal crosstalk. Furthermore, the parametric study is performed to determine which of the fourteen parameters have the greatest impact on the performance of one heater CF-PCR device. The main objective of this parametric study is to distinguish between the parameters that are either critical to the chip performance or can be freely specified. It is found that substrate thickness, flow rate, channel spacing, aspect ratio, channel pass length and external heat transfer coefficient are the most limiting parameters that can either improve or deteriorate the chip’s thermal performance. Overall, the impact of design and operating parameters are observed to be least on thermocycling profile at low Reynolds number (≤0.37 Re). However, in addition to the primary metric advantages of CF-PCR, one heater chip design helps in minimizing the thermal crosstalk effects by a factor of 4 in comparison to dual heater PCR while still maintaining a critical criteria of chip flexibility in terms of handling various sizes of DNA fragments. Hence, the proposed scheme paves the way for low-cost point-of-care diagnostics, system integration, and device miniaturization, realizing a portable microfluidic device applicable for on-site and direct field uses.

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The heat is on: room temperature affects laboratory equipment–an observational study

Cited by 19 publications

References 15 publications

Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens

Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens

Temperature variations within and between incubators—a prospective, observational study

Numerical Investigation of Design and Operating Parameters of Thermal Gradient Continuous-Flow PCR Microreactor Using One Heater

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