The healing of colonic anastomoses after early intraperitoneal chemotherapy: An experimental study in rats

Hillan, K J; Nordlinger, Bernard; Ballet, François; Puts, J. P.; Infante, R

doi:10.1016/0022-4804(88)90045-5

Cited by 51 publications

(43 citation statements)

References 8 publications

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“…Deterioration of the morphometric features which was caused by 5-fluorouracil and interferon did not correlate with increased frequency of the anastomotic leakage in our study, which was consistent with the results obtained by Hillan et al [38]. However, anastomotic leakage occurred in three animals treated with dexamethasone.…”

Section: Discussionsupporting

confidence: 92%

The use of morphometric and fractal parameters to assess the effects of 5-fluorouracil, interferon and dexamethasone treatment on colonic anastomosis healing: an experimental study in rats

Łętowska-Andrzejewicz¹,

Torres²,

Torres³

et al. 2011

Folia Histochem Cytobiol.

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Adjuvant chemotherapy and steroid therapy have been demonstrated to interfere with the wound healing process. The aim of this study was to evaluate the effects of 5-fluorouracil, interferon, and dexamethasone, on the healing of colon anastomosis by assessing morphometric and fractal parameters of the colonic wall. An experimental anastomosis of the ascending colon was performed in 60 male Wistar rats, which were then randomly assigned to four groups. On the second to sixth post-operative days, the rats were administered 5-fluorouracil, interferon-a, dexamethasone, or 0.9% NaCl solution as a control. Macroscopic, histomorphometric and microbiological evaluation was performed in order to assess healing of the anastomosis. In three animals from the dexamethasone group, there was leakage of anastomosis; adhesion formation was highest in the interferon group, and significantly higher than in the control and 5-fluorouracil groups. Histomorphometric parameter alterations were most pronounced on the seventh and fourteenth post-operative days in all treatment groups, with submucosal thickness the most affected parameter. Connective tissue fractal dimension was significantly decreased in those animals treated with interferon and dexamethasone. All three pharmaceutical agents impaired healing of anastomosis, and promoted infection in the anastomosis and skin wound sites. As dexamethasone induced both morphometric and macroscopic alterations, it was considered the most detrimental in this study.

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Section: Discussionsupporting

confidence: 92%

The use of morphometric and fractal parameters to assess the effects of 5-fluorouracil, interferon and dexamethasone treatment on colonic anastomosis healing: an experimental study in rats

Łętowska-Andrzejewicz¹,

Torres²,

Torres³

et al. 2011

Folia Histochem Cytobiol.

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“…Preoperatively administered cytotoxic agents may allow tumour resectability and decrease the incidence of distant or local metastases [26] . 5-FU can be injected IV and hepatic concentrations can also be achieved intraportally and IP [22,27,28] . In addition, intravenous administration may not allow sufficient penetration in the abdominal cavity.…”

Section: Discussionmentioning

confidence: 99%

Effect of early preoperative 5-fluorouracil on the integrity of colonic anastomoses in rats

Özel

Özel²,

Toros³

et al. 2009

WJG

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AIM:To determine the effect of chemotherapy on wound healing by giving early preoperative 5-fluorouracil (5-FU) to rats with colonic anastomoses. METHODS:Sixty Albino-Wistar male rats (median weight, 235 g) were used in this study. The rats were fed with standard laboratory food and given tap water ad libitum . The animals were divided into three groups:Group 1: Control group (chemotherapy was not administered), Group 2: Intraperitoneally (IP) administered 5-FU group (chemotherapy was administered IP to animals at a dose of 20 mg/kg daily during the 5 d preceeding surgery), Group 3: Intravenously (IV) administered 5-FU group. Chemotherapy was administered via the penil vein, using the same dosing scheme and duration as the second group. After a 3-d rest to minimize the side effects of chemotherapy, both groups underwent surgery. One centimeter of colon was resected 2 cm proximally from the peritoneal reflection, then sutured intermittently and subsequently end-to-end anastomosed. In each group, half the animals were given anaesthesia on the 3rd postoperative (PO) day and the other half on the 7th PO day, for in vivo analytic procedures. The abdominal incisions in the rats were dissected, all the new and old anastomotic segments were clearly seen and bursting pressures of each anastomotic segment, tissue hydroxyproline levels and DNA content were determined to assess the histologic tissue repair process. RESULTS:When the IV group was compared with the IP group, bursting pressures of the anastomotic segments on the 3rd and 7th PO days, were found to be significantly decreased, hydroxyproline levels at the anastomotic segment on the 7th PO day were significantly decreased (P < 0.01). CONCLUSION:In this study, we conclude that early preoperative 5-FU, administered IV, negatively affects wound healing. However, IP administered 5-FU does not negatively affect wound healing.

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“…In an experimental study of Hilan et al (27), it has been reported that the best criteria for the evaluation of the anastomotic healing of the colon are the macroscopic necrotomic findings. To assess the strength of colonic anastomoses, the most reliable method is considered to be the measurement of their bursting pressure (28,29).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Iloprost in the Healing of Colonic Anastomosis in Rats under Chemotherapy with Irinotecan

Arapoglou¹,

Kambaroudis²,

Grivas³

et al. 2017

chr

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RezumatScop: Am investigat posibilele efecte pozitive ale iloprostului în vindecarea anastomozelor colonice, la şobolani sub chimioterapie intraperitoneală cu irinotecan. Metodă: Patruzeci de şobolani masculi Wistar au fost împărţiţi în patru grupuri. În fiecare caz s-a practicat o colectomie parţială segmentară cu anastomoză termino-terminală. Ulterior li s-a administrat intraperitoneal, ser fiziologic (grupul 1), irinotecan (grupul 2), iloprost (grupul 3), irinotecan şi iloprost (grupul 4). Etapele următoare au constat în: autopsie, examinare macroscopică şi măsurarea presiunii de ruptură anastomotică. Un fragment al anastomozei a fost trimis pentru examen histologic şi pentru determinarea nivelurilor de hidroxiprolină. Rezultate: Frecvenţa fistulei anastomotice, cât şi a formării de aderenţe intraperitoneale au fost semnificativ crescute în grupul 2 comparativ cu celelalte grupuri. Presiunea de liză a anastomozei a fost semnificativ mai scăzută în grupul 2, comparativ cu toate celelalte grupuri; de asemenea, acest indicativ a fost semnificativ crescut în grupul 4 comparativ cu grupul 2. Gradul de leucocitoză, numărul de fibroblasti, nivelele de neocollagen şi hidroxiprolină din grupul 4 au fost semnificativ crescute comparativ cu grupul

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The healing of colonic anastomoses after early intraperitoneal chemotherapy: An experimental study in rats

Cited by 51 publications

References 8 publications

The use of morphometric and fractal parameters to assess the effects of 5-fluorouracil, interferon and dexamethasone treatment on colonic anastomosis healing: an experimental study in rats

The use of morphometric and fractal parameters to assess the effects of 5-fluorouracil, interferon and dexamethasone treatment on colonic anastomosis healing: an experimental study in rats

Effect of early preoperative 5-fluorouracil on the integrity of colonic anastomoses in rats

The Effect of Iloprost in the Healing of Colonic Anastomosis in Rats under Chemotherapy with Irinotecan

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