The HC-Pro and P3 Cistrons of an Avirulent <i>Soybean mosaic virus</i> Are Recognized by Different Resistance Genes at the Complex <i>Rsv1</i> Locus

Wen, R.-H.; Khatabi, Behnam; Ashfield, Tom; Maroof, M. A. Saghai; Hajimorad, M. R.

doi:10.1094/mpmi-06-12-0156-r

Cited by 64 publications

(91 citation statements)

References 74 publications

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“…TIRonly and TIR-X sequences were not included in this study. For R-genes with CNV, the expression analysis result and related traits reported in previous studies [25][26][27][28][77][78][79] were listed in Supplementary Table 23. …”

Section: Methodsmentioning

confidence: 99%

“…The G. soja genome contained a broader range of NBS R-gene domain architectures than did G. max, but G. max had more NBS-encoding genes (460 versus 334 in GsojaA to 382 in GsojaD) (Supplementary Table 22). R-genes showed more copy number loss in G. soja accessions (88 lost versus 33 gained and 8 copy number gain/loss, Supplementary Table 23), several of which were known to confer resistance to soybean mosaic virus 25 , Asian soybean rust 26 and Phytophthora root rot 27 , and be involved in legume-rhizobia symbiosis 28 . In addition, five structurally diverged regions (located on chromosomes 03, 06, 07 and 18) within cultivated G. max 29 , had a high frequency of CNV in the pan-genome (Supplementary Fig.…”

Section: Npgmentioning

confidence: 99%

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De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits

et al. 2014

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Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the establishment and analysis of a pan-genome of Glycine soja, the wild relative of cultivated soybean Glycine max, by sequencing and de novo assembly of seven phylogenetically and geographically representative accessions. Intergenomic comparisons identified lineage-specific genes and genes with copy number variation or large-effect mutations, some of which show evidence of positive selection and may contribute to variation of agronomic traits such as biotic resistance, seed composition, flowering and maturity time, organ size and final biomass. Approximately 80% of the pan-genome was present in all seven accessions (core), whereas the rest was dispensable and exhibited greater variation than the core genome, perhaps reflecting a role in adaptation to diverse environments. This work will facilitate the harnessing of untapped genetic diversity from wild soybean for enhancement of elite cultivars.

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Section: Methodsmentioning

confidence: 99%

Section: Npgmentioning

confidence: 99%

De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits

et al. 2014

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“…In many cases, R proteins interact with VSRs that also act as Avr factors, leading to the activation of defense responses (Cooley et al, 2000;de Ronde et al, 2013;Wen et al, 2013;Boualem et al, 2016). Intriguingly, some host factors involved in counter-counter defense against VSRs have been identified.…”

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confidence: 99%

A Violaxanthin Deepoxidase Interacts with a Viral Suppressor of RNA Silencing to Inhibit Virus Amplification

et al. 2017

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RNA silencing plays a critical role against viral infection. To counteract this antiviral silencing, viruses have evolved various RNA silencing suppressors. Meanwhile, plants have evolved counter-counter defense strategies against RNA silencing suppression (RSS). In this study, the violaxanthin deepoxidase protein of maize (Zea mays), ZmVDE, was shown to interact specifically with the helper component-proteinase (HC-Pro; a viral RNA silencing suppressor) of Sugarcane mosaic virus (SCMV) via its mature protein region by yeast two-hybrid assay, which was confirmed by coimmunoprecipitation in Nicotiana benthamiana cells. It was demonstrated that amino acids 101 to 460 in HC-Pro and the amino acid glutamine-292 in ZmVDE mature protein were essential for this interaction. The mRNA levels of ZmVDE were down-regulated 75% to 65% at an early stage of SCMV infection. Interestingly, ZmVDE, which normally localized in the chloroplasts and cytoplasm, could relocalize to HC-Pro-containing aggregate bodies in the presence of HC-Pro alone or SCMV infection. In addition, ZmVDE could attenuate the RSS activity of HC-Pro in a specific protein interaction-dependent manner. Subsequently, transient silencing of the ZmVDE gene facilitated SCMV RNA and coat protein accumulation. Taken together, our results suggest that ZmVDE interacts with SCMV HC-Pro and attenuates its RSS activity, contributing to decreased SCMV accumulation. This study demonstrates that a host factor can be involved in secondary defense responses against viral infection in monocot plants.

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“…This is mainly due to lack of knowledge on the role of NP gene of SVNaV in interaction with soybean. In other viral pathosystems, the significance of a single amino acid substitution in viral encoded proteins is well documented [31,32]. Collectively, these observations suggest that we used different soybean genotypes as well as a representative diverse virus population and demonstrated lack of seed transmissibility of SVNaV in soybean.…”

Section: Discussionmentioning

confidence: 76%

Evaluation of Seed Transmissibility of Soybean Vein Necrosis-Associated Virus in Two Soybean Cultivars Grown Under Field Conditions

Halter¹

2015

J Plant Pathol Microbiol

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Soybean vein necrosis-associated virus (SVNaV), originally found in Tennessee in 2008, has recently been reported from other soybean-growing regions of the United States as well as Canada. To provide direct evidence for lack of seed transmissibility of SVNaV in soybean, cultivars CF386RR2y/stsn and AG4832 were planted in a field in Kentucky during the 2013 growing season. Typical SVNaV induced symptoms emerged in early August. Tissues were collected from 150 symptomatic and asymptomatic plants of both cultivars and assayed by ELISA. SVNaV was detected in 94% of symptomatic, but none of asymptomatic plants. Seeds were harvested independently from 10 selected infected plants of each cultivar that exhibited the highest ELISA readings. A total of 2085 seeds from both cultivars were planted under greenhouse conditions and subsequently 1955 asymptomatic seedlings were harvested 4-5 weeks post-emergence and assayed individually by ELISA. Sap from only one of the seedlings exhibited an absorbance reading higher than background. However, when all the seeds from the same mother plant were grown and tested, none was positive. This suggests higher than background absorbance value for this single seedling is an anomaly. To search for probable genetic variation among SVNaV isolates from infected mother plants, whose seeds were subjected to grow out test, nucleocapsid protein gene was RT-PCR amplified from 13 mother plants representing both cultivars and sequenced. The analysis of sequences revealed the presence of distinct variants. Lack of potential for SVNaV to be seed transmissible in soybean is discussed.

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The HC-Pro and P3 Cistrons of an Avirulent Soybean mosaic virus Are Recognized by Different Resistance Genes at the Complex Rsv1 Locus

Cited by 64 publications

References 74 publications

De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits

De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits

A Violaxanthin Deepoxidase Interacts with a Viral Suppressor of RNA Silencing to Inhibit Virus Amplification

Evaluation of Seed Transmissibility of Soybean Vein Necrosis-Associated Virus in Two Soybean Cultivars Grown Under Field Conditions

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