The harmonized INFOGEST in vitro digestion method: From knowledge to action

Egger, Lotti; Ménard, Olivia; Delgado-Andrade, Cristina; Alvito, Paula; Assunção, Ricardo; Balance, Simon; Barberá, Reyes; Brodkorb, André; Cattenoz, Thomas; Clemente, Alfonso; Comi, Irene; Dupont, Didier; García‐Llatas, Guadalupe; Lagarda, Marı́a Jesús; Feunteun, Steven Le; JanssenDuijghuijsen, Lonneke; Karakaya, Sibel; Lesmes, Uri; Mackie, Alan; Martins, Carla; Meynier, Anne; Miralles, Beatriz; Murray, Brent S.; Pihlanto, Anne; Picariello, Gianluca; Santos, Cláudia N.; Şimşek, Şebnem; Recio, Isidra; Rigby, Neil M.; Rioux, Laurie Eve; Stoffers, Helena; Tavares, Ana; Tavares, Lucélia; Turgeon, Sylvie L.; Ulleberg, Ellen Kathrine; Vegarud, Gerd Elisabeth; Vergères, Guy; Portmann, Reto

doi:10.1016/j.foodres.2015.12.006

Cited by 191 publications

(79 citation statements)

References 28 publications

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“…These differences mainly concern the number of steps included in digestion (digestion taking place in mouth, stomach and small intestine) and composition of digestive fluids (enzymes, salts, buffers, compounds lowering surface tension) . Very recently, a new, unified, and harmonised in vitro digestion model (INFOGEST) was proposed . However, because of the complexity of faithful reproduction of the physicochemical and physiological phenomena occurring in a gastrointestinal tract, the results obtained in in vitro models might differ from those that actually take place in vivo .…”

Section: Resultsmentioning

confidence: 94%

Proteomic analysis of Lupinus angustifolius (var. Zeus and Bojar) and Lupinus luteus (var. Lord and Parys) seed proteins and their hydrolysates

et al. 2017

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The results of the present study provide insight into the nature of the digestion process that may take place after lupin seed protein intake and highlight the important fact that some of the proteins are insensitive to digestive enzyme activity. Moreover, evaluation of digestion activity of trypsin towards lupin seed proteins may be used for the development of specific processes with respect to hypoallergenic food production. © 2017 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 94%

Proteomic analysis of Lupinus angustifolius (var. Zeus and Bojar) and Lupinus luteus (var. Lord and Parys) seed proteins and their hydrolysates

et al. 2017

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“…Static digestion models are available which consist of combinations of simulated gastrointestinal proteolysis processes, including a gastric phase, a duodenal phase, and sometimes an oral phase (Mandalari et al, 2009;Minekus et al, 2014). A recent international consensus method described by Minekus et al (2014) and later validated by three inter-laboratory trials using skimmed milk powder demonstrates the importance of applying standardized methods allowing data comparison and discussion across laboratories (Egger et al, 2016).…”

Section: Enzymatic Degradation Assaysmentioning

confidence: 99%

Approaches to assess IgE mediated allergy risks (sensitization and cross-reactivity) from new or modified dietary proteins

Remington¹,

Broekman

Blom³

et al. 2018

Food and Chemical Toxicology

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A B S T R A C TThe development and introduction of new dietary protein sources has the potential to improve food supply sustainability. Understanding the potential allergenicity of these new or modified proteins is crucial to ensure protection of public health. Exposure to new proteins may result in de novo sensitization, with or without clinical allergy, or clinical reactions through cross-reactivity.In this paper we review the potential of current methodologies (in silico, in vitro degradation, in vitro IgE binding, animal models and clinical studies) to address these outcomes for risk assessment purposes for new proteins, and especially to identify and characterise the risk of sensitization for IgE mediated allergy from oral exposure. Existing tools and tests are capable of assessing potential crossreactivity. However, there are few possibilities to assess the hazard due to de novo sensitization. The only methods available are in vivo models, but many limitations exist to use them for assessing risk. We conclude that there is a need to understand which criteria adequately define allergenicity for risk assessment purposes, and from these criteria develop a more suitable battery of tests to distinguish between proteins of high and low allergenicity, which can then be applied to assess new proteins with unknown risks.

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“…This has led to great variability in scientific efforts, including some contradicting studies, and stimulated the recent effort of the INFOGEST network of scientists to develop a consensus harmonized static in vitro digestion model based on physiologically relevant conditions gathered from humans (Minekus et al, 2014). This harmonized protocol was validated in a wide inter-laboratory trial (Egger et al, 2016) and is currently pending on-going efforts to correlate findings of protein digestibility with an in vivo trial in pigs and biochemical assays with human aspirates (yet to be published). However, these and other numerous scientific publications focus on IVD systems designed for evaluating the digestive fate of foods and oral formulations in the adult alimentary canal.…”

Section: In Vitro Models For Food Researchmentioning

confidence: 99%

Extending in vitro digestion models to specific human populations: Perspectives, practical tools and bio-relevant information

Shani-Levi

Alvito

Andrés

et al. 2017

Trends in Food Science & Technology

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138

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a b s t r a c tBackground: In vitro digestion models show great promise in facilitating the rationale design of foods. This paper provides a look into the current state of the art and outlines possible future paths for developments of digestion models recreating the diverse physiological conditions of specific groups of the human population. Scope and approach: Based on a collective effort of experts, this paper outlines considerations and parameters needed for development of new in vitro digestion models, e.g. gastric pH, enzymatic activities, gastric emptying rate and more. These and other parameters are detrimental to the adequate development of in vitro models that enable deeper insight into matters of food luminal breakdown as well as nutrient and nutraceutical bioaccessibility. Subsequently, we present an overview of some new and emerging in vitro digestion models mirroring the gastro-intestinal conditions of infants, the elderly and patients of cystic fibrosis or gastric bypass surgery. Key findings and conclusions: This paper calls for synchronization, harmonization and validation of potential developments in in vitro digestion models that would greatly facilitate manufacturing of foods tailored or even personalized, to a certain extent, to various strata of the human population.

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The harmonized INFOGEST in vitro digestion method: From knowledge to action

Cited by 191 publications

References 28 publications

Proteomic analysis of Lupinus angustifolius (var. Zeus and Bojar) and Lupinus luteus (var. Lord and Parys) seed proteins and their hydrolysates

Proteomic analysis of Lupinus angustifolius (var. Zeus and Bojar) and Lupinus luteus (var. Lord and Parys) seed proteins and their hydrolysates

Approaches to assess IgE mediated allergy risks (sensitization and cross-reactivity) from new or modified dietary proteins

Extending in vitro digestion models to specific human populations: Perspectives, practical tools and bio-relevant information

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