The hamster cheek pouch model for field cancerization studies

Monti-Hughes, Andrea; Aromando, Romina F.; Pérez, Miguel A.; Schwint, Amanda E.; Itoiz, M. E.

doi:10.1111/prd.12066

Cited by 32 publications

(19 citation statements)

References 128 publications

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“…Four major types of in vivo models have been developed to observe the angiogenic process in two dimensions: the rat mesentery window assay (Norrby, 2011 ), the hamster cheek pouch assay (Monti-Hughes et al, 2015 ), the dorsal skinfold chamber adapted to mice, hamsters and rats (Lehr et al, 1993 ; Harder et al, 2014 ; Irvin et al, 2014 ), and the rabbit ear chamber assay (Clark et al, 1931 ; Ichioka et al, 1997 ). These techniques, developed as early as in the 1940s, rely on semi-transparent tissue or the implantation of a transparent chamber that allows an easy and direct visualization and quantification of the angiogenic process, including blood vessel density and blood flow velocity.…”

Section: In Vivo Modelsmentioning

confidence: 99%

Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration

Chávez¹,

et al. 2016

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Angiogenesis is the process through which new blood vessels are formed from preexisting ones and plays a critical role in several conditions including embryonic development, tissue repair and disease. Moreover, enhanced therapeutic angiogenesis is a major goal in the field of regenerative medicine and efficient vascularization of artificial tissues and organs is one of the main hindrances in the implementation of tissue engineering approaches, while, on the other hand, inhibition of angiogenesis is a key therapeutic target to inhibit for instance tumor growth. During the last decades, the understanding of cellular and molecular mechanisms involved in this process has been matter of intense research. In this regard, several in vitro and in vivo models have been established to visualize and study migration of endothelial progenitor cells, formation of endothelial tubules and the generation of new vascular networks, while assessing the conditions and treatments that either promote or inhibit such processes. In this review, we address and compare the most commonly used experimental models to study angiogenesis in vitro and in vivo. In particular, we focus on the implementation of the zebrafish (Danio rerio) as a model to study angiogenesis and discuss the advantages and not yet explored possibilities of its use as model organism.

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Section: In Vivo Modelsmentioning

confidence: 99%

Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration

Chávez¹,

et al. 2016

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“…Hamster buccal pouch carcinogenesis induced by painting with different carcinogenic agents in the oral mucosa has been widely studied for determination of histochemical, genetic, and biomolecular changes through the disease [7][8][9]. Given the similarity of the carcinogenesis process between humans and hamsters, this animal model is an important tool for research in oral oncology [10].…”

Section: Introductionmentioning

confidence: 99%

DMBA-Induced Oral Carcinoma in Syrian Hamster: Increased Carcinogenic Effect by Dexamethasone Coexposition

Gómez²,

Castro

et al. 2020

BioMed Research International

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Objectives. To investigate the effect of systemic administration of the immunosuppressant dexamethasone (DM) while inducing hamster buccal pouch DMBA carcinogenesis. Materials and Methods. Two different experiments were performed. In the first experiment, hamsters’ right buccal pouches in group A (n = 10) were painted three times per week with 7,12-dimethylbenzanthracene (DMBA) 0.5%, while pouches of animals in group B (n = 4) were painted with mineral oil only. Two animals were sacrificed every three weeks to obtain histological samples and to evaluate pathological abnormalities. After 12 weeks of exposition and with no macroscopic evidence of neoplasms, dexamethasone DM (1 mg/kg) was administered daily for 7 days to the last two animals in the study. In the second experiment, DM was administered since DMBA exposition, following the same protocol as the first experiment. Results. The time of macroscopic neoplasm development was reduced when DM-DMBA coexposition was employed, finding tumors after 10–12 weeks of exposition. In addition, the frequency of histopathological lesions was higher. Conclusion. Immunomodulatory action of dexamethasone may reduce the time of oral squamous cell carcinoma (OSCC) induction and may increase the incidence of neoplasms developed.

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“…In addition, the absorption of compounds from the cheek pouch into the systemic circulation is slow, which simulates the use of smokeless tobacco in human [12]. The hamster cheek pouch model has been widely used in oral cancer studies [13,14], but to our knowledge there are no reports about the microbiota of the cheek pouch and its application in studying the effects of toxicants on the oral microbiota. To this end, metagenomics sequencing, using the Illumina MiSeq platform and QIIME pipeline tools, was used to identify and classify bacterial microbiota present in the cheek pouches of hamsters upon smokeless tobacco exposure.…”

Section: Introductionmentioning

confidence: 99%

Smokeless tobacco impacts oral microbiota in a Syrian Golden hamster cheek pouch carcinogenesis model

et al. 2018

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The use of smokeless tobacco products (STPs) can cause many serious health problems. The oral microbiota plays important roles in oral and systemic health, and the disruption in the oral microbial population is linked to periodontal disease and other health problems. To assess the impact of smokeless tobacco on oral microbiota in vivo, high-throughput sequencing was used to examine the oral microbiota present in Syrian Golden hamster cheek pouches. Sixteen hamsters were divided into four groups and treated with the STP Grizzly snuff (0, 2.5, 25, or 250 mg) twice daily for 4 weeks. After 0, 1, 2, 3, and 4 weeks of treatment, bacterial genomic DNA was extracted from oral swabs sampled from the cheek pouches of the hamsters. The oral bacterial communities present in different hamster groups were characterized by sequencing the hypervariable regions V1-V2 and V4 of 16S rRNA using the Illumina MiSeq platform. Fifteen phyla, 27 classes, 59 orders, 123 families, and 250 genera were identified from 4,962,673 sequence reads from the cheek pouch samples. The bacterial diversity and taxonomic abundances for the different treatment groups were compared to the non–treated hamsters. Bacterial diversity was significantly decreased after 4 weeks of exposure to 2.5 mg, and significantly increased by exposure to 250 mg STP. Treatment with 250 mg STP significantly increased Firmicutes, transiently increased Cyanobacteria and TM7, and decreased Bacteroidetes and Fusobacteria compared to the control group. At the genus level, 4 weeks of administration of 250 mg STP significantly increased Granulicatella, Streptococcus, Oribacterium, Anaerococcus, Acidaminococcus, Actinomyces, Eubacterium, Negativicoccus, and Staphylococcus, and decreased Bacteroides, Buleidia, Dialister, and Leptotrichia, and transiently decreased Arcanobacterium compared to the control group. For the first time, an animal model was used for evaluating the effects of STP on oral microbiota by metagenomic sequencing. Our results provide a view of the shift of the oral microbiota in response to STP exposure in Syrian Golden hamster. Our findings indicate that the use of smokeless tobacco significantly disrupts the oral microbiota.

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The hamster cheek pouch model for field cancerization studies

Cited by 32 publications

References 128 publications

Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration

Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration

DMBA-Induced Oral Carcinoma in Syrian Hamster: Increased Carcinogenic Effect by Dexamethasone Coexposition

Smokeless tobacco impacts oral microbiota in a Syrian Golden hamster cheek pouch carcinogenesis model

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