The halothane sensitivity locus and its linkage relationships

Archibald, Alan; Imlah, P.

doi:10.1111/j.1365-2052.1985.tb01478.x

Cited by 45 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hal locus determines the disposition of a pig to develop stress syndrome including malignant hyperthermia (MH) under different stress conditions (for a review see Archibald & Imlah 1985). Malignant hyperthermia in humans has also been shown to be genetically controlled and it is considered to be homologous to porcine MH (Gronert 1980); however, nothing is yet known about the chromosomal localization or linkage relations of gene(s) controlling susceptibility to MH in humans (see e.g.…”

Section: Resultsmentioning

confidence: 99%

Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α₁B‐glycoprotein

1987

View full text Add to dashboard Cite

Pig, horse and dog plasma proteins, separated by horizontal polyacrylamide gel electrophoresis (pH 9.0) and electrophoretically transferred to nitrocellulose membranes, were tested for cross-reaction with antiserum to human plasma alB-glycoprotein (alB). The results showed that one previously reported polymorphic plasma postalbumin in each of these species (pig P02, horse Xk and dog Pa protein) was homologous to human plasma alB. In the light of the previously known genetic linkages in these species, this implied: (1) alB gene is close linked to Phi, Pgd and Hal (halothane sensitivity locus) loci in pigs; and (2) alB gene is linked to ME1 and Phi loci in horses. This suggested that the alB gene may also be found to be closely linked to gene(s) controlling susceptibility to malignant hyperthermia in humans and other mammals.

show abstract

Section: Resultsmentioning

confidence: 99%

Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α₁B‐glycoprotein

1987

View full text Add to dashboard Cite

show abstract

“…MH in pigs is controlled by a single major locus denoted the halothane sensitivity locus ( H A L ) found in a linkage group on chromosome 6 including the following loci: S(A-0)-GPI,HAL-H-A2 BG-PGD (reviewed by Archibald & Imlah 1985); the order of the GPI and HAL loci in this linkage group has yet to be determined. The CRC gene is expected to be included in this linkage group, located close to GPI, on the basis of the recent chromosomal assignment by in situ hybridization (Harbitz et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Multiple restriction fragment length polymorphisms in the porcine calcium release channel gene (CRC): assignment to the halothane (HAL) linkage group

et al. 1992

View full text Add to dashboard Cite

Two cDNA probes for the porcine calcium release channel gene (CRC) were used in restriction fragment length polymorphism (RFLP) analysis in an attempt to develop genetic markers linked to the malignant hyperthermia (stress susceptibility) gene ( H A L ) . Three TaqI RFLPs, denoted CRC1-CRC3, each composed of two alleles, were detected. RFLPs were also detected with MspI and PvuII, but the Msp I RFLP correlated completely with CRC3 in this material and the PvuII RFLP could not be scored reliably due to a minute size difference between the two allelic fragments. The autosomal codominant inheritance of these RFLP loci was confirmed by family analyses. Significant evidence for genetic linkage between the CRClICRC3 loci and the A l B G locus in the H A L linkage group confirmed a previous assignment of the CRC gene to chromosome 6 in the pig.

show abstract

“…The general approach is to look for co-segregation of alleles at a marker locus and phenotypicvariation for the trait of interest. Such linkage has been detected between the gene causing susceptibility to porcine stress syndrome and biochemical and blood group markers and catl be used as an aid to discriminating between homozygous and heterozygous animals (Archibald & Imlah 1985).…”

Section: Introductionmentioning

confidence: 99%

Use of DNA fingerprints for the detection of major genes for quantitative traits in domestic species

Haley

1991

Animal Genetics

View full text Add to dashboard Cite

The detection of marker loci linked to major genes or quantitative trait loci (QTL) of large effect in farm animal populations is of great potential value, both because it allows the easy manipulation of the major genes and because it provides a possible route to their ultimate isolation. At present the number of markers available is limited in farm animals. DNA fingerprints provide a promising source of informative marker loci and have the advantage that several loci can be detected on a single Southern hybridization. The disadvantage of DNA fingerprints is the difficulty in determining allelism of DNA fingerprint bands in different pedigrees and the fact that not all potentially resolvable loci can be resolved in a single pedigree. With probes capable of detecting 50 randomly distributed loci, about 50% of the genome of a typical domestic mammal might be expected to be closely linked to a marker (at a distance of 0-2 Morgans or less). If a proportion of DNA fingerprint loci prove to be clustered near chromosomal telomeres or elsewhere in the genome, coverage will be less. In order to detect linkage to a major gene, sires known or suspected to be heterozygous are used to produce large half-sibships, all animals in the pedigree are DNA fingerprinted and the phenotypes of the offspring are recorded. Where several heterozygous sires are available, sires can be selected in an attempt to maximize the number of marker loci resolved. The optimum number of sires needed to produce pedigrees will depend upon the size of the major gene, the number of DNA fingerprint probes available and the characteristics of the DNA fingerprints produced, but often one or two pedigrees will be optimum. Monte Carlo simulation was used to explore the power of detection of linkage between a major gene and a marker locus in a backcross. Maximum likelihood and analysis of variance of mean differences between marker genotypes were of similar power, but maximum likelihood provided reasonable estimates of the major gene effect and its linkage to the marker under some circumstances. One hundred offspring informative for the segregation of a marker would provide reasonable power for the detection of a gene causing a difference between the heterozygote and the homozygote of at least one within-sire, within-genotype standard deviation when linkage was very close (0-05 or less). With looser linkage (0.2 Morgans or less), a sample of the same size 259 260 C. S. Halev has reasonable power to detect genes causing at least two within-sire. withingenotype standard deviations difference between the heterozygote and the homozygote. The Booroola gene causes a difference of approximately 2-75 within-sire, within-genotype standard deviations for ovulation rate between heterozygous carriers and homozygous non-carriers of the Booroola allele. DNA fingerprints can provide a worthwhile probability of finding a marker linked to the Booroola gene in a sample of less than 100 informative offspring.

show abstract

The halothane sensitivity locus and its linkage relationships

Cited by 45 publications

References 49 publications

Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α₁B‐glycoprotein

Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α₁B‐glycoprotein

Multiple restriction fragment length polymorphisms in the porcine calcium release channel gene (CRC): assignment to the halothane (HAL) linkage group

Use of DNA fingerprints for the detection of major genes for quantitative traits in domestic species

Contact Info

Product

Resources

About

The halothane sensitivity locus and its linkage relationships

Cited by 45 publications

References 49 publications

Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α1B‐glycoprotein

Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α1B‐glycoprotein

Multiple restriction fragment length polymorphisms in the porcine calcium release channel gene (CRC): assignment to the halothane (HAL) linkage group

Use of DNA fingerprints for the detection of major genes for quantitative traits in domestic species

Contact Info

Product

Resources

About

Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α₁B‐glycoprotein

Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α₁B‐glycoprotein