THE HAIRLESS MOUSE and PHOTOAGING

Kligman, Lorraine H.

doi:10.1111/j.1751-1097.1991.tb02134.x

Cited by 50 publications

(26 citation statements)

References 49 publications

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“…differences in stratum corneum and epidermal thickness) between UVB-exposed skin and non-exposed skin [4,[26][27][28], one would expect that the penetration of ALA into the UVBexposed skin would be decreased as compared to nonexposed skin. Therefore, the results from the present study indicate that the chronic UVB exposure not only induces epidermal hyperplasia, but also alters the barrier function of the stratum corneum.…”

Section: Resultsmentioning

confidence: 99%

“…The stratum corneum and the dysplastic epidermis of chronically UVB-exposed skin is several layers of keratinocytes thicker than the normal stratum corneum and epidermis of normal nonexposed skin [4,[26][27][28]. As full thickness skin is used in the in vitro percutaneous penetration model, the passage of ALA through chronically UVB-exposed skin is across a longer distance than through normal non-exposed skin, resulting in a delayed accumulation of ALA in the receptor compartment underneath the chronically UVB-exposed skin.…”

Section: Resultsmentioning

confidence: 99%

“…One half was stripped 15 times with Scotch ß tape in order to reduce the stratum corneum, the other half was not treated. From the dorsal skin of chronically UVB-exposed hairless mice, an area without visible lesions, but with dysplastic and thickened epidermis [4,[26][27][28], was selected. The selected skin areas were excised and any adhering subcutaneous fat was carefully removed.…”

Section: In Vitro Percutaneous Penetration Modelmentioning

confidence: 99%

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Chronic UVB exposure enhances in vitro percutaneous penetration of 5‐aminulevulinic acid in hairless mouse skin

et al. 2004

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: In Vitro Percutaneous Penetration Modelmentioning

confidence: 99%

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Chronic UVB exposure enhances in vitro percutaneous penetration of 5‐aminulevulinic acid in hairless mouse skin

et al. 2004

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“…It has been established that many ofthe UVR-induced changes in the hairless mouse correlate well with those occurring in human photoaged skin [36], As trials of tretiiioin-treated human skin approach and exceed 2 years, it is becoming apparent that the hairless mouse model is also a predictive one for the repair of human photoaging. Recent reports have demonstrated a tretinoininduced subepidermal repair zone of new collagen [19,37], along with reduction in the abundant glycosaminoglycans present in photoaged skin [37], The ultrastructural reappearance of type VII collagen anchoring fibrils has been reported after 2 years of treatment [21], Most recently, Griffiths et al [22] demonstrated increased intracellular and extracellular type I collagen synthesis in the papillary dermis after 12 months of tretinoin treatment.…”

Section: Discussionmentioning

confidence: 99%

Topical Tretinoin Increases the Tropoelastin and Fibronectin Content of Photoaged Hairless Mouse Skin

Schwartz¹,

Kligman

1995

Journal of Investigative Dermatology

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Topical tretinoin treatment of photoaged hairless mice has been shown in previous studies to stimulate formation of a subepidermal zone of new connective tissue characterized by enhanced collagen synthesis. The aims of this study were to localize and/or quantify elastin, fibronectin, and glycosaminoglycans in the same model. Hairless mice (Skh-1) were irradiated thrice weekly for 10 weeks with gradually increasing doses of ultraviolet (up to 4.5 minimal erythema doses per exposure) from Westinghouse FS-40 bulbs. Mice were then treated five times a week with either 0.05% tretinoin, the ethanol:propylene glycol vehicle, or nothing for another 10 weeks. Controls included mice sacrificed after 10 weeks of ultraviolet treatment and age-matched untreated animals. The distribution of elastin and fibronectin was examined by immunofluorescence microscopy, which revealed fine fibrils in the subepidermal zone in tretinoin-treated skin. A quantitative slot-blot immunobinding assay showed that tretinoin induced a threefold higher amount of tropoelastin compared with controls. Insoluble elastin content (desmosine levels) was similar in all groups. Although fibronectin content was increased by ultraviolet radiation, tretinoin treatment induced the largest increase. In contrast, the amount of glycosaminoglycans, although increased by UVB radiation, was reduced by tretinoin treatment.

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“…Even though the concentration of air polluting substances may usually be low, they might have an adverse effect especially at sufficiently long exposure time and in highly polluted areas. Also, there is a risk of formation of free radicals resulting in deteriorating changes of the lipid fraction, potentially creating damage to the epidermis and dermis leading to premature wrinkling and sagging of the skin in exposed areas [7][8][9]. Since a great part of skin lipids are unsaturated derivatives [10], the radical-induced oxygenation can lead to formation of peroxide products, which are responsible for premature skin ageing.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative cosmetic additives for the protection of human skin surface lipids against environmental stress

Kroyer¹

1999

Fett/Lipid

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Studies were carried out in vitro to investigate the effect of a simulated polluted atmosphere containing ozone, sulfur dioxide, and nitrogen dioxide on human skin surface lipids and to evaluate the potency of antioxidative cosmetic additives for the protection against detrimental oxidation processes caused by air polluting substances. The experiments showed that the air pollutants caused significantly increased oxidation effects on human skin surface lipids compared to non polluted atmosphere as indicated by the increase of the peroxide values. The addition of selected derivatives of ascorbic acid, tocopherol, or retinol as specific cosmetic additives with particular antioxidative activity both as pure substances and as ingredients of cosmetic skin care formulations resulted in a considerable protection against oxidative effects of the air pollutants on human skin surface lipids, in which the antioxidative complex tocopherol/hydrogenated tallow glyceride citrate is most effective. Even by additional irradiation with artificial erythemogenic sunlight during the exposure to the air polluting substances of a skin care formulation composed of tocopherol/hydrogenated tallow glycerides as antioxidative component and zinc oxide as UV‐filter substance, the significant lipid‐protective efficacy of the antioxidative cosmetic additive was only slightly diminished.

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THE HAIRLESS MOUSE and PHOTOAGING

Cited by 50 publications

References 49 publications

Chronic UVB exposure enhances in vitro percutaneous penetration of 5‐aminulevulinic acid in hairless mouse skin

Chronic UVB exposure enhances in vitro percutaneous penetration of 5‐aminulevulinic acid in hairless mouse skin

Topical Tretinoin Increases the Tropoelastin and Fibronectin Content of Photoaged Hairless Mouse Skin

Antioxidative cosmetic additives for the protection of human skin surface lipids against environmental stress

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