THE HAEMAGGLUTININ OF <i>HAEMOPHILUS PERTUSSIS</i>.

Keogh, E. V.; North, E. A.

doi:10.1038/icb.1948.33

Cited by 41 publications

(18 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relationship betwpon snnnn antihaemap;p;liitinin level and protection of mice against intranasally introdueed experimental pertussis has been pointed out in previons papers (Keogh and North, 1948;Fisher, 19.50); the lowest mouse sernm titre at whieh consistent and firm protection conld be demonstrated was about 16 (Fisher and Warbnrton,195.0;Warbnrton and Fisher,19.51).…”

Section: Discussionmentioning

confidence: 97%

The Haemagglutinin of Haemophilus Pertussis

Fisher¹,

Warburton²,

Wettenhall³

et al. 1951

Aust J Exp Biol Med

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 97%

The Haemagglutinin of Haemophilus Pertussis

Fisher¹,

Warburton²,

Wettenhall³

et al. 1951

Aust J Exp Biol Med

View full text Add to dashboard Cite

“…The virulent strains P.6] and P.71 were used. The properties of these strains were described by Keogh and North (1948), and by Fisher (1948), respectively. These strains have been maintained in the lyophilized state, from which the organisms were sown on to Bordet Gengou medium and subcultured on the latter for routine use for periods not exceeding four weeks, when a fresh ampoule of dried culture was opened.…”

Section: Mat'erials and Methodsmentioning

confidence: 99%

“…Kaemagglutinin titrations were carried out according to the method of Keogh and North (1948). Titres are expressed as the reciprocal of the highest dilution giving complete agglutination.…”

Section: Mat'erials and Methodsmentioning

confidence: 99%

“…Keogh and North (1948) showed that the virulence of pertussis strains for mice as judged by the intranasal technique (Burnet and Timmins, 1937), is related to their haemagglutinin content, and they demonstrated that protection of mice against intranasal infection depends on the haemagglutinin content of the vaccines, and the antihaemagglutinin level of sera in active and passive methods of immunization, respectively.The present paper deals with observations made on the nature and composition of the haemagglutinating principle present in supernatants of fluid cultures. …”

mentioning

confidence: 99%

“…The course of immunization was then continued with material prepared in the same way as for the first injection, but the route of administration was changed to the subcutaneous one. Six injections were given over three weeks and the rabbit bled one week after the last dose.Kaemagglutinin titrations were carried out according to the method of Keogh and North (1948). Titres are expressed as the reciprocal of the highest dilution giving complete agglutination.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Haemagglutinin of Haemophilus Pertussis

Fisher¹

1950

Aust J Exp Biol Med

View full text Add to dashboard Cite

Keogh, North and Warburton (1947) reported the presence of a haemagglutinin in H. pertussis. Keogh and North (1948) showed that the virulence of pertussis strains for mice as judged by the intranasal technique (Burnet and Timmins, 1937), is related to their haemagglutinin content, and they demonstrated that protection of mice against intranasal infection depends on the haemagglutinin content of the vaccines, and the antihaemagglutinin level of sera in active and passive methods of immunization, respectively.The present paper deals with observations made on the nature and composition of the haemagglutinating principle present in supernatants of fluid cultures. MAT'ERIALS AND METHODS.strains and enltwres. The virulent strains P.6] and P.71 were used. The properties of these strains were described by Keogh and North (1948), and by Fisher (1948), respectively. These strains have been maintained in the lyophilized state, from which the organisms were sown on to Bordet Gengou medium and subcultured on the latter for routine use for periods not exceeding four weeks, when a fresh ampoule of dried culture was opened. The fluid medium used was that described by Cohen and Wheeler (1946), modified by raising the content of soluble starch to 1 p.c. The medium was dispensed in 130 to 150 ml. lots in Eoux bottles which were seeded heavily from 24-hour Bordet Gengou slope cultures of the organism. The bottles were laid flat on their sides in the 37° C. incubator and left undisturbed for three days. Only heavily grown cultures were used. After checking for purity by smear, the organisms were deposited on the angle centrifuge, the supernatant separated and stored in the cold room following the addition of 0-01 p.c. merthiolate.The reference serum, B4. An English rabbit was immunized against the erythrocyteadsorbable components of pertussis supernatant as follows: 5 ml. of the rabbit's own red cells washed in saline, were suspended in 30 ml. supernatant, allowed to stand for 15 minutes, centrifuged out, washed 3 times in saline, and re-injected in saline suspension into the animal's ear vein. The rabbit developed severe toxic symptoms, but recovered after a few days. The course of immunization was then continued with material prepared in the same way as for the first injection, but the route of administration was changed to the subcutaneous one. Six injections were given over three weeks and the rabbit bled one week after the last dose.Kaemagglutinin titrations were carried out according to the method of Keogh and North (1948). Titres are expressed as the reciprocal of the highest dilution giving complete agglutination. Human group "O" cells were used in all tests involving haemagglutination.

show abstract

Rheumatic Fever Activity Determination by Two Correlative Methods

SKILLMAN¹

1955

Arch Intern Med

View full text Add to dashboard Cite

Two methods were employed in a study to ascertain whether the identification of a specific bacterial antigen or antibodies activated by it could be the means of determining rheumatic fever activity. The first method was by a hemolytic test with patient's serum as the antibody source and artificially sensitized sheep erythrocytes as the antigen. The second method was by an agglutination test using rabbit antiserum and patient's erythrocytes sensitized in vivo as the antigen. Clinical correlations were obtained with 78 rheumatic fever patients. There were 77 control cases. The methods proved practical in ascertaining rheumatic fever activity or quiescence and in distinguishing rheumatic fever from other disease processes.The principle of a specific antigen-antibody reaction has been the basis of numerous tests to identify infectious diseases.* Erythrocytes that have been coated or sensitized by an antigen react with a specific antiserum prepared from that antigen to produce agglutination of the coated red cells.\s=d\ When complement is added to this reaction, hemolysis occurs under certain conditions. Because the manifestations of rheumatic fever are asso¬ ciated with Group A beta-hemolytic Strep¬ tococcus, it was the purpose of this study to ascertain whether a means of identifying a specific bacterial antigen, or the antibodies elicited by it, could likewise determine rheu¬ matic fever activity. It was postulated that the bacteria producing this tissue response possess a common antigen which is responsi¬ ble for the manifestations of rheumatic fever. METHODS OF STUDYTwo methods of determination were employed in this study. To demonstrate the presence of a specific antibody in the serum of rheumatic fever patients, sheep red cells were coated with heat-killed Group A beta-hemolytic streptococci, which had been iso¬ lated as the actual etiologic agents in active rheu¬ matic fever patients. The coated red cells were then incubated with each patient's serum, and comple¬ ment was added to produce hemolysis when the patient's serum contained the specific antibodies.The second method employed Group A betahemolytic Streptococcus antiserum obtained by im¬ munizing rabbits. The rheumatic fever patient's red cells were added to this antiserum to produce ag¬ glutination when the patient's cells were coated with the specific antigen. Preparation of Antigen MediaBrain-heart infusion (Difco product), 37 gm., was dissolved in 1000 cc. of distilled water and sterilized in the autoclave at 15 lb. pressure (121 C) for 20 minutes. If the medium was not to be used the same day that it was sterilized, it was placed in flowing steam or boiling water for a few moments to drive off dissolved gases and cooled without agi¬ tation. Part of the brain-heart infusion was dis¬ pensed into small test tubes to which freshly washed sterile sheep cells were added so that each tube con¬ tained 10 cc. of a 0.5% cell suspension. The remain¬ ing brain-heart infusion was dispensed into larger test tubes to which sterile horse serum was added so that eac...

show abstract

THE HAEMAGGLUTININ OF HAEMOPHILUS PERTUSSIS.

Cited by 41 publications

References 3 publications

The Haemagglutinin of Haemophilus Pertussis

The Haemagglutinin of Haemophilus Pertussis

The Haemagglutinin of Haemophilus Pertussis

Rheumatic Fever Activity Determination by Two Correlative Methods

Contact Info

Product

Resources

About