The gut microbiome composition and degradation enzymes activity of black Amur bream (<i>Megalobrama terminalis</i>) in response to breeding migratory behavior

Liu, Yaqiu; Li, Xinhui; Li, Jie; Chen, Weitao

doi:10.1002/ece3.7407

Cited by 17 publications

(18 citation statements)

References 54 publications

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“…It is well established that microbiota play essential roles in host nutrition, physiology, and health [6,9]. However, little is known about the composition, diversity, and function of the microbiota associated with different environmental compartments and habitats in juvenile A. schlegelii.…”

Section: Discussionmentioning

confidence: 99%

“…The gut microbiota have been shown to affect the metabolic capacity of the fish host and to have beneficial effects on host nutrition, growth, reproduction, and health [5][6][7][8][9][10][11]. Hence, studies have increasingly focused on exploring the fish-gut microbiome, with the long-term goal of developing healthy and sustainable aquaculture production systems [12].…”

Section: Introductionmentioning

confidence: 99%

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Relationships between the Gut Microbiota of Juvenile Black Sea Bream (Acanthopagrus schlegelii) and Associated Environment Compartments in Different Habitats

et al. 2021

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The fish-gut microbiota play a key role in the physiology, development, and fitness of its host. An understanding of fish-gut microbial communities and the factors influencing community composition is crucial for improving fish performance. In this study, we compared the gut microbiota of juvenile black sea bream Acanthopagrus schlegelii among habitats: (1) wild, (2) offshore cage-culture, and (3) pond-culture. We also explored the relationships between the gut microbiota and host-associated environmental factors. Gut samples and associated environmental compartments were investigated using 16S rRNA gene sequencing. Our results revealed significant habitat-specific differences among the gut microbiota of juvenile A. schlegelii. Wild populations of juvenile A. schlegelii had more diverse gut microbiota than populations cultured in pond habitats due to their omnivorous feeding habits and the corresponding abundance of natural food resources. Significant variations in the composition, core taxa, and diversity of the microbiota were also found between the gut and the environmental compartments. However, no significant differences were observed among the microbiota of the environmental compartments in the relatively isolated pond habitat. Source tracking analysis recovered connections between the fish-gut microbiota and the diet, water and sediment environmental compartments. This connection was especially strong between the microbiota of the fish gut and that of the diet in the pond habitat: the diet microbiota accounted for 33.48 ± 0.21% of the gut microbiota. Results suggested that all A. schlegelii shared a core gut microbiota, regardless of differences in diet and habitat. However, environmental factors associated with both diet and habitat contributed to the significant differences between the gut microbiota of fish living in different habitats. To the authors’ knowledge, this study presents the first comparison of gut microbiota among juvenile A. schlegelii with different diets and habitats. These findings enrich our understanding of the gut microbiota of A. schlegelii and help to clarify the interaction between gut microbiota and environmental factors. Our results may also help to guide and improve fish ecological fitness via the regulation of gut microbiota, thereby increasing the efficacy of stock enhancement programs for this species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relationships between the Gut Microbiota of Juvenile Black Sea Bream (Acanthopagrus schlegelii) and Associated Environment Compartments in Different Habitats

et al. 2021

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“…Several studies have established that fish gut microbial community compositions are distinct among different species, even among those co-habiting in the same environment ( Eichmiller et al, 2016 ; Baldo et al, 2017 ). Differentiation of the fish gut microbiome can frequently be predicted by host variable exogenous diet, environmental conditions, and phylogeny ( Fishelson et al, 1985 ; Su et al, 2020 ; Liu et al, 2021 ). In this study, we preliminarily investigated the relationship between cyprinid fish hosts CO1 genetic distance and gut microbiome dissimilarity.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiomes of cyprinid fish exhibit host-species symbiosis along gut trait and diet

Liu

et al. 2022

Front. Microbiol.

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Teleost omnivorous fish that coexist partially sharing resources are likely to modify their gut traits and microbiome as a feedback mechanism between ecological processes and evolution. However, we do not understand how the core gut microbiome supports the metabolic capacity of the host and regulates digestive functions in specialized omnivorous fish gut traits. Therefore, we evaluated the gut microbiome of eight omnivorous fish from a single family (i.e., Cyprinidae) in the current study. We examined the correlation between host phylogeny, diet composition, and intestinal morphological traits related to the intestinal microbiome. The results indicated that cyprinid fish with similar relative gut lengths had considerable gut microbiome similarity. Notably, the SL (short relative gut length) group, as zoobenthos and zooplankton specialists, was abundant in Proteobacteria and was less abundant in Firmicutes than in the ML (medium relative gut length) and LL (long relative gut length) groups. These fish could extract nutrients from aquatic plants and algae. Additionally, we found the relative abundance of Clostridium and Romboutsia to be positively correlated with host relative gut length but negatively correlated with the relative abundance of Cetobacterium, Plesiomonas, Bacteroides, and Lactobacillus, and host-relative gut length. We also show a positive linear relationship between host gut microbiome carbohydrate metabolism and relative gut length, while the amino acid and lipid metabolism of the gut microbiome was negatively correlated with host-relative gut length. In addition, omnivorous species competing for resources improve their ecological adaptability through the specialization of gut length, which is closely related to variation in the synergy of the gut microbiome. Above all, specialized gut microbiota and associated gut morphologies enable fish to variably tolerate resource fluctuation and improve the utilization efficiency of nutrient extraction from challenging food resources.

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“…The results of this study showed Proteobacteria and Firmicutes phyla as typical dominant flora in the gut of the four fish species. Proteobacteria and Firmicutes are two phyla typical dominant flora in the intestine of many fish, such as Oncorhynchus mykiss (Ingerslev et al, 2014), Nibea coibor and Nibea diacanthus (Li et al, 2019), Megalobrama terminalis (Liu et al, 2021), Micropterus salmoides (Zhou et al, 2021) andSymphysodon haraldi (Zhang et al, 2021). The four fish species had variations in abundant of microbiota species.…”

Section: Discussionmentioning

confidence: 99%

“…The intestine tract is the main site for digestion and nutritional uptake, which has been regarded as a key organ in fish nutrition (Kumar et al, 2005;Wang et al, 2018;Zhou et al, 2021). The fish digestive enzymes activities are closely related to the diet consumed and the ability of fish to digest and absorb different nutrients (Bakke et al, 2010;Liu et al, 2021). Evidently, previous studies found herbivorous fish such as Roho labeo (Labeo rohita ) and Japanese eel (Anguilla japonica ) had stronger amylase activity compared to carnivorous fish such as Great white catfish (Wallago attu ) (Agrawal et al, 1975) and rainbow trout (Oncorhynchus mykiss ) (Hidalgo et al, 1999).…”

mentioning

confidence: 99%

A comparison of digestive strategies for fishes with different feeding habits: digestive enzyme activities, intestinal morphology and gut microbiota

Jiao

Zhang²,

Limbu

et al. 2022

Preprint

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Feeding habit determines the digestive tract structure and intestinal microflora. However, the relationship among feeding habit, digestive physiology intestinal, and microbial diversity of omnivorous, herbivorous, filter-feeder and carnivorous fish reared in the same pond has not been compared. This study compared the digestive enzyme activities, intestinal morphology and intestinal microflora of omnivorous (Carassius auratus), herbivorous (Ctenopharyngodon idellus), carnivorous (Siniperca chuatsi) and filter-feeder (Shizothorax grahami) and predicted the potential functions of specific microflora on different nutrients. Twelve intestine samples were collected from each of the four fishes from Dianchi Lake. The composition and diversity of microbial communities were determined by using high throughput sequencing of 16S rDNA. The results showed that the filter-feeder fish had significantly higher protease but lower amylase activities in the intestine than herbivorous. The carnivorous fish intestine had more microvilli branches and complex structures than other fish species in the order carnivorous > herbivorous > filter-feeder > omnivorous. The diversity of intestinal microflora was higher in omnivorous and followed the order omnivorous > herbivorous > filter-feeder > carnivorous. Acinetobacter species and Bacteroides species were the most dominant flora in carnivorous and herbivorous fish, respectively. Acinetobacter johnsonii, Acinetobacter lwoffii and Pseudomonas stutzeri might help the host to digest protein, while Bacteroidetes species may help the host to digest cellulose. Taken together, feeding habit determines the digestive enzyme activities, intestinal tissue morphology and differential colonization of fish intestinal flora. The knowledge obtained is useful in designing appropriate approaches for feed formulation and feeding practices in for fish.

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The gut microbiome composition and degradation enzymes activity of black Amur bream (Megalobrama terminalis) in response to breeding migratory behavior

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 17 publications

References 54 publications

Relationships between the Gut Microbiota of Juvenile Black Sea Bream (Acanthopagrus schlegelii) and Associated Environment Compartments in Different Habitats

Relationships between the Gut Microbiota of Juvenile Black Sea Bream (Acanthopagrus schlegelii) and Associated Environment Compartments in Different Habitats

Gut microbiomes of cyprinid fish exhibit host-species symbiosis along gut trait and diet

A comparison of digestive strategies for fishes with different feeding habits: digestive enzyme activities, intestinal morphology and gut microbiota

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