The Growth of Transplanted Liver Cells Within the Pancreas

Jaffe, V; Darby, H.; Selden, Clare; Hodgson, H. J. F.

doi:10.1097/00007890-198802000-00053

Cited by 25 publications

(6 citation statements)

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“…Although several previous studies (reviewed in refs. 1 and 2) have used histological techniques to demonstrate hepatocyte-like cells within the spleen (3)(4)(5)(6)(7)(8)(9)(10)(11)(12), fat pads (13), pancreas (14,15), or subrenal capsule (16), or on microcarrier beads in the peritoneum (17), after hepatocyte injections, the number of surviving cells and their degree of liver function have been impossible to assess due to the absence of quantitative biochemical markers and/or rejection of transplanted cells. Short-term (4,(17)(18)(19)(20) or long-term (3,14) reconstitution of some hepatic function has been demonstrated by transplanting normal hepatocytes into rats with a genetic deficiency of uridine diphosphate (UDP)-glucuronyltransferase, but it is unclear how many surviving hepatocytes were required to produce the slight decrease in serum bilirubin and increase in conjugated bilirubin in the bile.…”

mentioning

confidence: 99%

Mouse hepatocytes migrate to liver parenchyma and function indefinitely after intrasplenic transplantation.

Ponder

Gupta

Leland

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

353

196

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One approach to gene therapy for hepatic diseass is to remove hepatocytes from an affected individual, genetically alter them in vitro, and reimplant them into a receptive locus. Although returning hepatocytes to the liver itself would be advantageous, the feasibility of this approach has never been evaluated due to the inability to distinguish donor from host hepatocytes. To unambiguously identify transplanted hepatocytes after transplantation, and to better quantitate their number and degree of liver function, two transgenic mouse lines were generated in a C57BL/6 background. The first expresses the Escherichia cofi p-galactosidase gene from the relatively liver-specific human a1-antitrypsin (hAAT) promoter and allows transgenic hepatocytes to be readily identified after 5-bromo-4-chloro-3-indolyl .8-D-galactoside staining; the second produces the hAAT protein under control of the same promoter, which enables hepatocyte survival and maintenance of liver function to be quantitated by measuring the serum levels of hAAT. Hepatocytes isolated from transgenic donors were transplanted into nontransgenic C57BL/6 recipients by intrasplenic injection. Surprisingly, a large fraction of these cells were identified within the liver parenchyma but not the spleen at 2 months after transplantation. The high levels of serum hAAT detected in transplant recipients were stable for >6 months, suggesting that established cells will survive indefinitely. These results have important implications for liver organogenesis and hepatic gene therapy.

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mentioning

confidence: 99%

Mouse hepatocytes migrate to liver parenchyma and function indefinitely after intrasplenic transplantation.

Ponder

Gupta

Leland

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

353

196

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“…After in vitro culture, the leaflet was implanted back into the lamb from which the cells were harvested, replacing one of the pulmonary valve leaflets. These studies 27,28 demonstrated appropriate function of the tissue-engineered leaflet, as determined by echocardiography, up to 11 weeks. Future investigations will be directed toward evaluating the long-term durability of the leaflets in vivo and tissue engineering an entire heart valve that can be used for replacement for diseased heart valves.…”

Section: Heart Valve and Cartilagementioning

confidence: 75%

Tissue Engineering

Kaihara

Vacanti²

1999

Arch Surg

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“…Hepatocyte survival in other ectopic sites has been varied. Hepatocytes survive in interscapular fat pads (25,26), beneath the renal capsule (271, intraperitoneally when attached to extracellular matrix components such as collagen-coated microcarrier beads (28) and in pancreas (29,30). When hepatocytes are transplanted into the pulmonary vascular bed (31) (33).…”

Section: /1/34222mentioning

confidence: 99%