The Growth of Micro-organisms in Relation to their Energy Supply

Bauchop, T.; Elsden, S. R.

doi:10.1099/00221287-23-3-457

Cited by 755 publications

(393 citation statements)

References 20 publications

(8 reference statements)

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“…Where, DG ATP is the energy released from ATP hydrolysis, DG ATP ¼ 30:53 kJ=mol ATP (Burton, 1958); MW cell is the molecular weight of cell mass, MW cell ¼ 26:4 g=mol À C based on the formula of CH 2 O 0.6 N 0.2 ; Y ATP is the bacteria yield per ATP, Y ATP ¼ 10:5 g dry weight/mol ATP according to the experimental results of Bauchop (Bauchop, 1958;Bauchop and Elsden, 1960); and 0.9 is the assumed percentage of organic material in cell dry weight (Rittmann and McCarty, 2001). With the above calculations completed, one final assumption is needed to create the energy balance.…”

Section: Energy Balancementioning

confidence: 99%

Expanded thermodynamic model for microbial true yield prediction

Xiao

VanBriesen

2005

Biotech & Bioengineering

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Thermodynamic methods to predict true yield and stoichiometry of bacterial reactions have been widely used in biotechnology and environmental engineering. However, yield predictions are often inaccurate for certain simple organic compounds. This work evaluates an existing method and identifies the cause of prediction errors for compounds with low degree of reductance of carbon. For these compounds, carbon, not energy or reducing equivalents, constrains growth. Existing thermodynamically-based models do not account for the potential of carbon-limited growth. The improved method described here consists of four balances: carbon balance, nitrogen balance, electron balance, and energy balance. Two efficiency terms, K1 and K2 are defined and estimated from a priori analysis. The results show that K1 and K2 are nearly the same in value so that only one coefficient, K ¼ 0.41 is used in the modified model. Comparisons with observed yields show that use of the new model and parameters results in significantly improved yield estimation based on inclusion of the carbon balance. The average estimation error is less than 6% for the data set presented.

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Section: Energy Balancementioning

confidence: 99%

Expanded thermodynamic model for microbial true yield prediction

Xiao

VanBriesen

2005

Biotech & Bioengineering

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“…L"sing the scheme of Costello et al, the overall rate of biomass production of glucose fermenters can be calculated from: generated (Bauchop and Elsden, 1960). The respective yields given by these workers for reactions 2.21 to 2.24 are four, three, two and two ATP molecules per molecule of glucose consumed.…”

Section: Cell Synthesismentioning

confidence: 99%

A mathematical model of a high sulphate wastewater anaerobic treatment system

Knobel

Lewis

2002

Water Research

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“…Estimates of YATP in anaerobic S. cerevisiae cultures average 10.5-11 g biomass.mol ATP formed -I (Bauchop & Elsden 1960;Korman~hikov~i et al 1969). However, these results were obtained from batch cultures, in which the YATP strongly depends on the time of harvesting as shown by Haukeli & Lie (1971).…”

Section: Atp Requirement For Anaerobic Chemostat Growth Of S Cerevismentioning

confidence: 99%

A theoretical evaluation of growth yields of yeasts

Verduyn

Stouthamer

Scheffers

et al. 1991

Antonie van Leeuwenhoek

211

176

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Growth yields of Saccharomyces cerevisiae and Candida utilis in carbon-limited chemostat cultures were evaluated. The yields on ethanol and acetate were much lower in S. cerevisiae, in line with earlier reports that site I phosphorylation is absent in this yeast. However, during aerobic growth on glucose both organisms had the same cell yield. This can be attributed to two factors: -S. cerevisiae had a lower protein content than C. utilis; -uptake of glucose by C. utilis requires energy whereas in S. cerevisiae it occurs via facilitated diffusion. Theoretical calculations showed that, as a result of these two factors, the ATP requirement for biomass formation in C. utilis is 35% higher than in S. cerevisiae (theoretical YATP values of 20.8 and 28.1, respectively). The experimental YATP for anaerobic growth of S. cerevisiae on glucose was 16 g biomass-mol ATP -1.In vivo P/O-ratios can be calculated for aerobic growth on ethanol and acetate, provided that the gap between the theoretical and experimental ATP requirements as observed for growth on glucose is taken into account. This was done in two ways: -via the assumption that the gap is independent of the growth substrate (i.e. a fixed amount of ATP bridges the difference between the theoretical and experimental values). -alternatively, on the assumption that the difference is a fraction of the total ATP expenditure, that is dependent on the substrate. Calculations of P/O-ratios for growth of both yeasts on glucose, ethanol, and acetate made clear that only by assuming a fixed difference between theoretical and experimental ATP requirements, the P/O-ratios are more or less independent of the growth substrate. These P/O-ratios are approximately 30% lower than the calculated mechanistic values.

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The Growth of Micro-organisms in Relation to their Energy Supply

Cited by 755 publications

References 20 publications

Expanded thermodynamic model for microbial true yield prediction

Expanded thermodynamic model for microbial true yield prediction

A mathematical model of a high sulphate wastewater anaerobic treatment system

A theoretical evaluation of growth yields of yeasts

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