The growth of micro-organisms in parenteral nutrition solutions containing amino acids and sugars

Holmes, C. J.; Allwood, M. C.

doi:10.1016/0378-5173(79)90039-5

Cited by 14 publications

(14 citation statements)

References 17 publications

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“…The results of 3 bacterial species in this study demonstrated that the acidity of the TPN solution is the critical factor suppressing the bacterial growth, but that the hyperosmolality of conventional TPN solutions has a little effect. On the other hand, the results of C. albicans in this study demonstrated that both the acidic pH and the hyperosmolality of TPN solutions hardly have an effect on Candida growth as reported in other studies 6,11,12…”

Section: Discussionsupporting

confidence: 77%

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Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid

Kubo¹,

Kaneda²,

Shimono³

et al. 2010

Int. J. Med. Sci.

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Background: To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (Staphylococcus aureus, Serratia marcescens, Bacillus cereus, and Candida albicans) in TPN solutions without lipid. Methods: Experiment 1: A commercial TPN solution without lipid containing multivitamins (pH5.6) was used. A specific number of each test microorganism was added to each 10 mL of the TPN solution and incubated at room temperature. An aliquot of test solution was sampled and inoculated to SCD agar plates at 0, 24, and 48 hrs after the addition of the microorganisms. The number of microorganisms was counted as colony forming units. Experiment 2: The other 2 commercial TPN solutions without lipid (pH5.5) were supplemented with multivitamins. The pH values of the solutions were adjusted to about 6.0, 6.5, or 7.0 using 0.5 mol/L NaOH. The addition of microorganisms, incubation, and counting were performed in the same manner. Results: Experiment 1: S. aureus, S. marcescens, and B. cereus did not increase in the TPN solution without lipid containing multivitamins (pH5.6), but C. albicans increased rapidly. Experiment 2: The 3 bacterial species did not increase even at pH6.0, but increased at pH6.5 and increased rapidly at pH7.0 in both TPN solutions. C. albicans increased similarly at any pH. Conclusion: These results suggest that bacterial species cannot grow in TPN solutions without lipid due to the acidity (pH5.6 or lower), but Candida species can grow regardless of the acidity.

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Section: Discussionsupporting

confidence: 77%

“…They estimated that the acidic pH and/or the hyperosmolality might suppress the bacterial growth 9,10,12. The results of 3 bacterial species in this study demonstrated that the acidity of the TPN solution is the critical factor suppressing the bacterial growth, but that the hyperosmolality of conventional TPN solutions has a little effect.…”

Section: Discussionmentioning

confidence: 61%

Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid

Kubo¹,

Kaneda²,

Shimono³

et al. 2010

Int. J. Med. Sci.

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“…Accordingly, the four formulations were challenged with microorganisms, incubated at a range of temperature (4-30°C) and viable counts made for up to 21 days. Previous studies (7,(12)(13)(14) have also investigated the ability of various TI" mixtures to support microbial growth, but studies have invariably been of a limited duration (i.e. up to 48 h.).…”

Section: Discussionmentioning

confidence: 99%

Microbial Contamination and Growth in Total Parenteral Nutrition Solutions

1988

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TPN bags (196) and giving sets were subjected to microbiological examination following administration within a busy nutrition ward. Of these, five (2.6%) were found to be contaminated with coagulase-negative Gram-positive cocci. In all but one instance the contamination was restricted to the terminal ends of the giving sets rather than to the nutrition bag itself. Isolation of micro-organisms from the ward environment suggested that the contamination had arisen extrinsically during the setting up of the infusions. Isolates from the contaminated products, together with type species of Escherichia coli and Candida albicans, were examined with respect to their growth requirements and used to challenge four TPN formulations. Growth and survival of the organisms was monitored for up to 21 days. In all instances significant numbers of organisms were recovered after 72 h. Significant growth of the Staphylococcal isolate and C. albicans occurred over the initial 48-72 h incubation, this appeared to be greater in extent for the lipid-containing formulations. The temperature of storage of the formulation was the major determining factor for microbial growth and survival. No survivors were recovered, however, from any formulation after 21 days.

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“…With an increase in the concentration of dextrose, bacterial growth may be inhibited (4,9). With an increase in the concentration of dextrose, bacterial growth may be inhibited (4,9).…”

Section: K Nordfeld M Rasmussen and V Gauna Jensenmentioning

confidence: 99%

Storage of Mixtures for Total Parenteral Nutrition—ii. Microbiological Control of Large Volume TPN Mixtures

1984

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SUMMARY Microbiological control of TPN mixtures can be performed either by controlling the aseptic filling procedure using culture medium instead of intravenous solutions, or by an end‐product sterility test. A microbiological process control of the filling procedure is described, and it is shown that a hospital pharmacy can prepare 3‐litre bags without contamination. The number of 3‐litre bags necessary in the process control is discussed, as well as the frequency with which a control should be performed. A possible non‐destructive system for testing each 3‐litre bag microbiologically is outlined and discussed. This system may be used when TPN mixtures without lipid emulsion are stored for longer periods.

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The growth of micro-organisms in parenteral nutrition solutions containing amino acids and sugars

Cited by 14 publications

References 17 publications

Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid

Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid

Microbial Contamination and Growth in Total Parenteral Nutrition Solutions

Storage of Mixtures for Total Parenteral Nutrition—ii. Microbiological Control of Large Volume TPN Mixtures

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