The Growth of Bordetella pertussis: a Review

Rowatt, Elizabeth

doi:10.1099/00221287-17-2-297

Cited by 36 publications

(15 citation statements)

References 32 publications

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“…In terms of media requirements, only glutamate, proline, cysteine, and nicotinic acid have been found to be essential for growth . However, beyond these minimal nutritional requirements, significant improved growth and antigens' yields can only be achieved by the addition of casaminoacids in the forms of yeast extract and hydrolyzed milk proteins. The variability of these raw materials is a source of batch‐to‐batch variability and serves as motivation to study the fermentation step with the goal of reducing this variability.…”

Section: Introductionmentioning

confidence: 99%

A dynamic metabolic flux balance based model of fed‐batch fermentation of bordetella pertussis

Budman

Patel

Tamer

et al. 2013

Biotechnology Progress

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A mathematical model based on a dynamic metabolic flux balance (DMFB) is developed for a process of fed-batch fermentation of Bordetella pertussis. The model is based on the maximization of growth rate at each time interval subject to stoichiometric constraints. The model is calibrated and verified with experimental data obtained in two different bioreactor experimental systems. It was found that the model calibration was mostly sensitive to the consumption or production rates of tyrosine and, for high supplementation rates, to the consumption rate of glutamate. Following this calibration the model correctly predicts biomass and by-products concentrations for different supplementation rates. Comparisons of model predictions to oxygen uptake and carbon emission rates measurements indicate that the TCA cycle is fully functional.

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Section: Introductionmentioning

confidence: 99%

A dynamic metabolic flux balance based model of fed‐batch fermentation of bordetella pertussis

Budman

Patel

Tamer

et al. 2013

Biotechnology Progress

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“…(58) found differences in the production of alkali from certain amides and organic salts, and these may prove useful after further evaluation. There is abundant evidence for retaining B. bronchiseptica in the same genus as B. pertussis and B. parapertussis on physiological, biochemical, serological, and genetic grounds (20,28,46,64,66). The question is, therefore, whether Alcaligenes should be united with Bordetella.…”

Section: Vol 231973mentioning

confidence: 99%

“…The three genera that would remain are phenotypically very similar. They have a marked preference for metabolizing amino acids (29,52,66). Two genera show a requirement for accessory growth factors: Bordetella requires nicotinamide and Brucella requires thiamine.…”

Section: Vol 231973mentioning

confidence: 99%

Taxonomy of Bordetella and Related Organisms of the Families Achromobacteraceae, Brucellaceae, and Neisseriaceae

Johnson

Sneath

1973

International Journal of Systematic Bacteriology

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A numerical taxonomic study was made of strains belonging to the genera Bordetella, Alcaligenes, Brucella, Acinetobacter, Moraxella, Neisseria, Haemophilus, Actinobacillus, and Pasteurella, together with a few strains from allied genera. They were examined for 139 characters covering a wide range of tests and properties. The strains fell into two major groups. Group A contained Haemophilus, Actinobacillus, and Pasteurella. Group B contained eight main clusters, representing, respectively, Bordetella pertussis, Bordetella parapertussis, Bordetella bronchiseptica combined with Alcaligenes faecalis, Brucella, Acinetobacter anitratus, Acinetobacter lwoffii, and two clusters containing Moraxella and Neisseria strains. Brucella and the three clusters of Bordetella (including Alcaligenes) were more similar to one another than t o the other clusters; it is suggested that the family Brucellaceae should comprise these three genera, and that the other genera should be placed elsewhere.Several families of bacteria listed in Bergey ' s ManuaI (13) represent aggregations of genera which at best can only be described as loose associations. Instead of encompassing organisms which overall are closely similar, they have been set up t o accommodate genera with one or two prominent characters in common. Such a group is Brucellaceae, or its predecessor Parvobacteriaceae (12,63). This family is the niche for those gram-negative, rod-shaped bacteria which require rich media for growth and are responsible for many human and animal infections. Their potential pathogenicity and the alleged difficulties of working with them account largely for their neglect by taxonomists. They tend to grow poorly or t o die out on the usual media, whereas the addition of blood t o increase growth often interferes with tests.Within Brucellaceae the genus Bordetella Moreno-L6pez 1952 has been one of the least studied. Three species are generally recognized: the type species B. pertussis (the whoopingcough bacillus), B. parapertussis, and B. bronchiseptica. Although these organisms have been placed in various genera (e-g., Haemophilus), in recent years their segregation as a separate genus has become generally accepted ( 1 3, 84). They do not require X factor (haematin) or V factor (nicotinamide adenine dinucleotide) as do species of Haemophilus. The only reason that B. pertussis requires blood is to remove toxic substances from growth media; the blood can be replaced by starch or charcoal (65) or by anionic-exchange resins (45).Bordetella-species are obligate aerobes, whereas species of Haernophilus are facultative anaerobes. The guanine plus cytosine (G + C ) ratios also differ, being 67 t o 70% for Bordetella and 38 to 42% for Haemophilus (20, 36, and findings reported below).The separation of Bordetella from Alcaligenes, Acinetobacter, and Brucella is more difficult to justify, and the main motive for the present work was to investigate this question. Many workers have noted points of similarity between Bordetella bronchiseptica and A k aligenes faecali...

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“…Several previous investigators (Ungar et al 1950;Rowatt 1957 ;MacLennan 1960) have published work based on the two phase IV NCTC strains, under the designation 154 E and 146 E. Rowatt considered them to be phase IV B. pertussis strains, more closely related to B. parapertussis than to other phase IV strains. She quoted a private communication from Dr. J. Ungar that the strains "grew on digest agar shortly after isolation from cases of whooping cough..…”

Section: Discussionmentioning

confidence: 99%

A reexamination of phase IV Bordetella pertussis

Aprile¹

1972

Can. J. Microbiol.

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Eight strains of Bordetella pertussis labeled as "phase IV" and received from different laboratories were examined for their cultural, biochemical, and immunological characteristics in comparison with four phase I strains. Five of the strains possessed cultural and biochemical characteristics agreeing with published information on phase IV B. pertussis. Two of the strains had properties more closely resembling those of Brucella melitensis and one resembled Lophomonas sp.None of the phase IV strains possessed the protective antigen (PA) or the histamine-sensitizing factor (HSF) and they showed little immunological relationship with one another when tested for agglutination by antisera to phase IV except for L-46, L-51, and L-84, which formed a homogeneous serological group. Certain phase I antisera showed some degree of cross-reactivity with phase IV strains but there was much variation between the individual phase IV strains. Conversely the phase IV antisera cross-reacted with phase I organisms.

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The Growth of Bordetella pertussis: a Review

Cited by 36 publications

References 32 publications

A dynamic metabolic flux balance based model of fed‐batch fermentation of bordetella pertussis

A dynamic metabolic flux balance based model of fed‐batch fermentation of bordetella pertussis

Taxonomy of Bordetella and Related Organisms of the Families Achromobacteraceae, Brucellaceae, and Neisseriaceae

A reexamination of phase IV Bordetella pertussis

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