The growth of Arabidopsis primary root is repressed by several and diverse amino acids through auxin-dependent and independent mechanisms and MPK6 kinase activity

Ravelo-Ortega, Gustavo; López‐Bucio, Jesús Salvador; Ruíz-Herrera, León Francisco; Pelagio‐Flores, Ramón; Ayala-Rodríguez, Juan Ángel; Cruz, Homero Reyes de la; Guevara-García, Ángel Arturo; López‐Bucio, José

doi:10.1016/j.plantsci.2020.110717

Cited by 10 publications

(3 citation statements)

References 60 publications

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“…The vertical bar indicates the standard error of the mean (n = 4). acids as low as 100 µM applied via root caused damage in the root growth (Ravelo-Ortega et al, 2021). Thus, low concentrations of amino acids in some plant species can have un-expected effects.…”

Section: Discussionmentioning

confidence: 99%

Physiological, Nutritional and Metabolomic Responses of Tomato Plants After the Foliar Application of Amino Acids Aspartic Acid, Glutamic Acid and Alanine

et al. 2021

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Agriculture is facing a great number of different pressures due to the increase in population and the greater amount of food it demands, the environmental impact due to the excessive use of conventional fertilizers, and climate change, which subjects the crops to extreme environmental conditions. One of the solutions to these problems could be the use of biostimulant products that are rich in amino acids (AAs), which substitute and/or complement conventional fertilizers and help plants adapt to climate change. To formulate these products, it is first necessary to understand the role of the application of AAs (individually or as a mixture) in the physiological and metabolic processes of crops. For this, research was conducted to assess the effects of the application of different amino acids (Aspartic acid (Asp), Glutamic acid (Glu), L-Alanine (Ala) and their mixtures Asp + Glu and Asp + Glu + Ala on tomato seedlings (Solanum lycopersicum L.). To understand the effect of these treatments, morphological, physiological, ionomic and metabolomic studies were performed. The results showed that the application of Asp + Glu increased the growth of the plants, while those plants that received Ala had a decreased dry biomass of the shoots. The greatest increase in the growth of the plants with Asp + Glu was related with the increase in the net CO2 assimilation, the increase of proline, isoleucine and glucose with respect to the rest of the treatments. These data allow us to conclude that there is a synergistic effect between Aspartic acid and Glutamic acid, and the amino acid Alanine produces phytotoxicity when applied at 15 mM. The application of this amino acid altered the synthesis of proline and the pentose-phosphate route, and increased GABA and trigonelline.

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Section: Discussionmentioning

confidence: 99%

Physiological, Nutritional and Metabolomic Responses of Tomato Plants After the Foliar Application of Amino Acids Aspartic Acid, Glutamic Acid and Alanine

et al. 2021

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“…Chinensis L.) [75]. Isolated AAs, including leucine (L-Leu), lysine (L-Lys), tryptophan (L-Trp), and glutamate (L-Glu), repress cell division and elongation in Arabidopsis primary roots [76], and its effect could be associated with auxin-and mitogen-activated protein kinase (MAPK) signaling [76,77]. All these findings indicate that PHs can improve the root system architecture, and future investigations will be performed to decipher regulatory molecular networks and understand cross-talks between hormones and peptides in PH-controlled root physiology.…”

Section: Root Architecturementioning

confidence: 99%

Biostimulant Properties of Protein Hydrolysates: Recent Advances and Future Challenges

Malécange

Sergheraert²,

Teulat

et al. 2023

IJMS

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Over the past decade, plant biostimulants have been increasingly used in agriculture as environment-friendly tools that improve the sustainability and resilience of crop production systems under environmental stresses. Protein hydrolysates (PHs) are a main category of biostimulants produced by chemical or enzymatic hydrolysis of proteins from animal or plant sources. Mostly composed of amino acids and peptides, PHs have a beneficial effect on multiple physiological processes, including photosynthetic activity, nutrient assimilation and translocation, and also quality parameters. They also seem to have hormone-like activities. Moreover, PHs enhance tolerance to abiotic stresses, notably through the stimulation of protective processes such as cell antioxidant activity and osmotic adjustment. Knowledge on their mode of action, however, is still piecemeal. The aims of this review are as follows: (i) Giving a comprehensive overview of current findings about the hypothetical mechanisms of action of PHs; (ii) Emphasizing the knowledge gaps that deserve to be urgently addressed with a view to efficiently improve the benefits of biostimulants for different plant crops in the context of climate change.

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“…The level of another amino acid, threonine, was found to be a limiting factor for root apical meristem maintenance in roots of A. thaliana (Reyes-Hernández et al 2019). A recent report demonstrated that several amino acids, including leucine, are perceived at the root apex and repress A. thaliana primary root growth by inhibition of both cell division and elongation (Ravelo-Ortega et al 2021). The authors revealed that leucine and few other amino acids diminish the auxin response around the stem cell niche and columella and antagonize auxin flux via reduced expression of the PIN auxin transporter proteins.…”

Section: Hypothetical Model About Atf-box Role In Root Developmentmentioning

confidence: 99%

<i>AtF-box</i> gene expression fine-tunes <i>Arabidopsis thaliana</i> root development

et al. 2021

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Root growth is under constant dynamic regulation for optimal response to developmental and environmental stimuli. At the posttranslational level, protein abundance is controlled by proteasomal degradation of targeted proteins. The substrate-specificity of this process is exerted by F-box proteins taking part in the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. In this work an Arabidopsis thaliana AtF-box, which regulates leucine homeostasis, was analyzed in the context of root development. Publicly available data sets and reporter lines revealed AtF-box expression in the primary and lateral roots. Aberrant stem cell divisions were detected in the distal stem cells (DSC) of the AtF-box knockdown lines (AtF-box amiRNA ), suggesting that AtF-box is required for the optimal cell division. Microscopic observations revealed the premature exit from cell proliferation and slower cell division activity. Conversely, in AtF-box overexpression (AtFbox OE ) lines the cell division phase was prolonged. The root growth rate was respectively reduced and enhanced in the AtF-box amiRNA and AtF-box OE lines compared to the control. From the results of these studies, we concluded that the AtF-box gene is important for the fine-tuning of root growth.

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The growth of Arabidopsis primary root is repressed by several and diverse amino acids through auxin-dependent and independent mechanisms and MPK6 kinase activity

Cited by 10 publications

References 60 publications

Physiological, Nutritional and Metabolomic Responses of Tomato Plants After the Foliar Application of Amino Acids Aspartic Acid, Glutamic Acid and Alanine

Physiological, Nutritional and Metabolomic Responses of Tomato Plants After the Foliar Application of Amino Acids Aspartic Acid, Glutamic Acid and Alanine

Biostimulant Properties of Protein Hydrolysates: Recent Advances and Future Challenges

<i>AtF-box</i> gene expression fine-tunes <i>Arabidopsis thaliana</i> root development

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