The Group III Two-Component Histidine Kinase AlHK1 is Involved in Fungicides Resistance, Osmosensitivity, Spore Production and Impacts Negatively Pathogenicity in Alternaria longipes

Luo, Yi‐Yong; Yang, Jinkui; Zhu, Ming; Liu, Chen Jian; Li, Hai Yan; Lu, Ze Bao; Pan, Wen Zheng; Zhang, Zhong Hua; Bi, Wei; Zhang, Ke Qin

doi:10.1007/s00284-012-0093-8

Cited by 24 publications

(17 citation statements)

References 20 publications

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“…Adversely, no fitness penalty was found associated with dicarboximide resistance (and phenylpyrrole sensitivity) in field strains (Oshima et al, 2002, 2006). Notably, in some fungal species, no developmental defect besides osmosensitivity was found associated with phenylpyrrole resistance (Motoyama et al, 2005; Luo et al, 2012). …”

Section: Resistance To Phenylpyrrolesmentioning

confidence: 99%

“…Field strains cross-resistant to phenylpyrroles and dicarboximides have been isolated from A. brassicicola, A. longipes , and A. alternata populations (Dry et al, 2004; Iacomi-Vasilescu et al, 2004; Avenot et al, 2005; Luo et al, 2012; Avenot and Michailides, 2015; Malandrakis et al, 2015). No significant developmental defects could be detected in the A. brassicicola resistant mutants and only moderate osmosensitivity (Avenot et al, 2005; Iacomi-Vasilescu et al, 2008).…”

Section: Resistance To Phenylpyrrolesmentioning

confidence: 99%

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Phenylpyrroles: 30 Years, Two Molecules and (Nearly) No Resistance

Kilani¹,

Fillinger²

2016

Front. Microbiol.

101

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Phenylpyrroles are chemical analogs of the natural antifungal compound pyrrolnitrin. Fenpiclonil, but mainly fludioxonil are registered against multiple fungal crop diseases since over 25 years for seed or foliar treatment. They have severe physiological impacts on the pathogen, including membrane hyperpolarization, changes in carbon metabolism and the accumulation of metabolites leading to hyphal swelling and burst. The selection and characterization of mutants resistant to phenylpyrroles have revealed that these fungicides activate the fungal osmotic signal transduction pathway through their perception by a typical fungal hybrid histidine kinase (HHK). The HHK is prone to point mutations that confer fungicide resistance and affect its sensor domain, composed of tandem repeats of HAMP motifs. Fludioxonil resistant mutants have been selected in many fungal species under laboratory conditions. Generally they present severe impacts on fitness parameters. Since only few cases of field resistance specific to phenylpyrroles have been reported one may suspect that the fitness penalty of phenylpyrrole resistance is the reason for the lack of field resistance.

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Section: Resistance To Phenylpyrrolesmentioning

confidence: 99%

Section: Resistance To Phenylpyrrolesmentioning

confidence: 99%

Phenylpyrroles: 30 Years, Two Molecules and (Nearly) No Resistance

Kilani¹,

Fillinger²

2016

Front. Microbiol.

101

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“…Resistance to PCNB has been documented in other ascomycete plant pathogens, including Botrytis cinerea and Sclerotinia sclerotiorum, as well as in Alternaria sp. and Magnaporthe oryzae (Steel & Nair, 1993;Bradley et al, 2006;Luo et al, 2012). Recently, Luo et al (2012) demonstrated that an osmosensitive signal transduction pathway present in many of these fungi governs resistance to both PCNB and dicarboximides.…”

Section: Discussionmentioning

confidence: 99%

“…and Magnaporthe oryzae (Steel & Nair, 1993;Bradley et al, 2006;Luo et al, 2012). Recently, Luo et al (2012) demonstrated that an osmosensitive signal transduction pathway present in many of these fungi governs resistance to both PCNB and dicarboximides. Whether the reduced sensitivity to PCNB in S. homoeocarpa isolate Heath1A in our in vitro assays correlates with practical resistance to this fungicide in the field or with crossresistance to dicarboximide fungicides is unknown, but additional studies with this isolate may be of interest.…”

Section: Discussionmentioning

confidence: 99%

Development of a semi-selective medium for improved isolation of the turfgrass dollar spot pathogenSclerotinia homoeocarpafrom host tissues

Rioux

Ryzin

Kerns

2014

Canadian Journal of Plant Pathology

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Dollar spot, caused by Sclerotinia homoeocarpa, is one of the most economically devastating diseases of amenity turfgrasses worldwide. The pathogen is readily isolated from active lesions, but detection from seed, dormant host tissue and other plant debris that may serve as a source of primary inoculum is difficult. A semi-selective medium was developed to enhance isolation of S. homoeocarpa. Various fungicides used on turfgrass, the pH indicator dye bromophenol blue, and two pH levels, were assessed for their effects on, and in the case of bromophenol blue, response to growth of S. homoeocarpa and contaminant fungi frequently isolated from field and seed samples. Amendment of the medium to pH 4 promoted growth of S. homoeocarpa in the absence of fungicides and enhanced pathogen growth relative to contaminant isolates on medium amended with 0.1 μg mL −1 triticonazole or 5 μg mL −1 azoxystrobin. The growth rate of S. homoeocarpa on these three media, as determined by in vitro radial growth assays, was consistent for many isolates representing the majority of S. homoeocarpa vegetative compatibility groups. Isolation efficiency on the pH-amended media was tested against antibiotic-amended potato dextrose agar from field samples and artificially inoculated turfgrass seed. In both cases, the medium amended to pH 4 and containing 5 μg mL −1 azoxystrobin significantly decreased contaminant growth with no adverse effects on recovery of S. homoeocarpa in comparison with antibiotic-amended PDA. The enhanced specificity of this medium will be a useful tool for selective detection of S. homoeocarpa and identification of sources of initial inoculum for dollar spot development.Résumé: La brûlure en plaques, causée par Sclerotinia homoeocarpa, est une des maladies du gazon d'agrément dont les répercussions économiques sont des plus importantes à l'échelle mondiale. L'agent pathogène est facilement isolé à partir de lésions actives, mais la détection à partir de semences, de tissu dormant et d'autres débris végétaux qui peuvent servir de source primaire d'inoculum n'est pas aussi simple. On a développé un milieu semi-sélectif pour mettre en relief l'isolement de S. homoeocarpa. Divers fongicides utilisés sur le gazon, l'indicateur de pH bleu de bromophénol et deux niveaux de pH ont été évalués quant à leurs effets sur S. homoeocarpa et, dans le cas du bleu de bromophénol, quant à la réaction par rapport à sa croissance ainsi qu'aux champignons contaminants fréquemment isolés d'échantillons de sol et de semences. L'amendement du milieu pour obtenir un pH de 4 a stimulé la croissance de S. homoeocarpa en l'absence de fongicides et a favorisé la croissance de l'agent pathogène par rapport aux isolats contaminants se développant sur le milieu amendé avec 0.1 µg mL −1 de triticonazole ou 5 µg mL −1 d'azoxystrobine. Le taux de croissance de S. homoeocarpa sur ces trois milieux, comme défini pas les biotests de croissance radiale in vitro, était uniforme pour plusieurs isolats représentant la majorité des groupes de compatibilité végét...

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“…The unique role for HK3 in sensing the fungicidal cue is similar to what has been learned from NikA, an HK3 homologue that was shown to play a major role in SakA (Hog1) phosphorylation in response to fludioxonil rather than to osmotic shock in A. fumigatus (Hagiwara et al ., ). However, the fungicide‐sensing role of HK3 is different from the reported roles of other Group III HKs (Os1/TcsC/Bos1/DrkA) in sensing both osmotic and fungicidal cues (Ochiai et al ., ; Liu et al ., ; McCormick et al ., ) or mediating the two hallmark phenotypes in several fungi, such as P. marneffei (Boyce et al ., ), Alternaria longipes (Luo et al ., ) and Fusarium oxysporum (Rispail and Di Pietro, ). Despite increased sensitivity to H 2 O 2 or menadione, the Δ hk3 cells triggered with or without H 2 O 2 showed the same signal of phosphorylated Hog1 as the cells of the control strains, suggesting no role for HK3 in sensing oxidative cue in B. bassiana .…”

Section: Discussionmentioning

confidence: 99%

Two histidine kinases can sense different stress cues for activation of the MAPK Hog1 in a fungal insect pathogen

Liu

Tong

Qiu

et al. 2017

Environmental Microbiology

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Filamentous fungi possess a large family of histidine kinases (HKs) classified to 11 distinct groups. Of those, only Group III (HK3) homologues in some fungi can sense osmotic, fungicidal and/or oxidative cues upstream of the signalling MAPK Hog1 cascade that usually governs two hallmark phenotypes, i.e., osmotolerance and dicarboximide/phenylpyrrole fungicide resistance. Here we examined functions of all eight HK groups with each possessing a single member in Beauveria bassiana. HK3 and HK8 (Group VIII) were evidently localized at hyphal cell periphery and had more functions in aerial conidiation and multiple stress responses than six other HKs. Increased osmosensitivity occurred uniquely in Δhk8 while high resistance to fludioxonil (phenylpyrrole) or iprodione (dicarboximide) fungicide appeared only in Δhk3. Phosphorylation of Hog1 was inhibited only in the Δhk3 cells triggered with fludioxonil cue and reduced only in the Δhk8 cells triggered with osmotic cue. However, an oxidative cue resulted in no signal change of phosphorylated Hog1 in either Δhk3 or Δhk8. Other Δhk mutants showed minor changes in some non-hallmark phenotypes. Altogether, HK3 and HK8 act as unique sensors of fungicidal and hyperosmotic cues respectively, upstream of the Hog1 cascade that regulates fungicidal resistance negatively and osmotolerance positively in B. bassiana.

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The Group III Two-Component Histidine Kinase AlHK1 is Involved in Fungicides Resistance, Osmosensitivity, Spore Production and Impacts Negatively Pathogenicity in Alternaria longipes

Cited by 24 publications

References 20 publications

Phenylpyrroles: 30 Years, Two Molecules and (Nearly) No Resistance

Phenylpyrroles: 30 Years, Two Molecules and (Nearly) No Resistance

Development of a semi-selective medium for improved isolation of the turfgrass dollar spot pathogenSclerotinia homoeocarpafrom host tissues

Two histidine kinases can sense different stress cues for activation of the MAPK Hog1 in a fungal insect pathogen

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