The green tea polyphenol (−)‐epigallocatechin gallate and green tea can protect human cellular DNA from ultraviolet and visible radiation‐induced damage

Morley, Nick; Clifford, Tim; Salter, Leo; Campbell, Sandra J.; Gould, David; Curnow, Alison

doi:10.1111/j.1600-0781.2005.00119.x

Cited by 116 publications

(73 citation statements)

References 25 publications

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“…Trabalhos desenvolvidos com o chá verde (Camellia sinensis), espécie vegetal com concentrações expressivas de polifenóis, demonstraram a atividade fotoprotetora deste metabólito Annie et al, 2005;Morley et al, 2005 As cumarinas, componentes da classe dos compostos fenólicos, apresentam espectro característico na ultravioleta, fortemente influenciado pela natureza da posição dos substituintes. Apesar de apresentar atividades relativamente limitadas na indústria farmacêutica, foi ponto de partida para a descoberta de alguns fármacos, como a varfarina (Cunha, 2005).…”

Section: Introductionunclassified

Avaliação in vitro da atividade fotoprotetora de extratos vegetais do cerrado de Mato Grosso

Violante¹,

Souza²,

Venturini³

et al. 2009

Rev. bras. farmacogn.

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RESUMO:O cerrado brasileiro é um bioma detentor de grande diversidade biológica. No entanto, são escassas as pesquisas de espécies vegetais, especialmente do cerrado mato-grossense, com potencial para serem utilizadas como filtros solares naturais. O objetivo desta pesquisa foi estudar o potencial fotoprotetor de espécies de diferentes famílias (Apocynaceae, Lythraceae, Oxalidaceae) do cerrado da região do Rio Manso, Chapada dos Guimarães -MT. A absorbância dos extratos etanólicos secos foram medidas em diferentes concentrações entre os comprimentos de onda de 260 a 400nm para verificar a absorção nas regiões ultravioleta A e B (UVA e UVB). As plantas que apresentaram absorbância na região estudada foram submetidas a uma análise fitoquímica qualitativa preliminar para determinar a presença de polifenóis e alcalóides, constituintes característicos de plantas que absorvem a radiação UV. Os extratos etanólicos secos que apresentaram absorção em UVB foram submetidos ao teste de determinação in vitro do Fator de Proteção Solar (FPS) desenvolvido por Mansur. M. Velame apresentou absorção na região UVB com absorbância máxima em 318nm, enquanto que a L. pacari e O. hirsutissima apresentaram absorbância na região UVA. Na concentração utilizada e padronizada, nenhuma das espécies apresentou FPS ≥2, sendo assim não podem ser consideradas plantas com potencial fotoprotetor.Unitermos: Apocynaceae, atividade fotoprotetora, Cerrado de Mato Grosso, fator de proteção solar in vitro, Lythraceae, Oxalidaceae.ABSTRACT: "In vitro sunscreen activity evaluation of plants extracts from Mato Grosso cerrado". The Brazilian savanna is a holding biome of large biological diversity. However, the researches of plants species are scarce, especially at the Mato Grosso´s savanna; which have potential to be used as natural sunscreen. The objective of this research was to study the photoprotector potential of several species (Apocynaceae, Lythraceae, Oxalidaceae) from the savanna´s region at the Manso River, Chapada dos Guimarães -MT. The absorbance of dry ethanolic extracts were measured in different concentrations, between waves from 260nm until 400 nm in length. Just to check the absorption in the A and B ultraviolet regions (UVA and UVB). The plants that presented absorbance by the studied area were submitted to a preliminary qualitative phytochemical analysis to determine if there are polyphenols and alkaloids inside, because they are typical constituents of plants that absorber the UV radiation. The dry ethanolic extracts, that presented absorption in UVB, were submitted to a in vitro Sun Protection Factor (SPF) determination test, developed by Mansur. M.velame presented absorption in the UVB region with maximal absorbance at 318 nm, while L. pacari and O. hirsutissima presented absorbance in the UVA region. At the used and standardized concentration, no species presented SPF ≥ 2, so they cannot be considered plants with photoprotector potential.

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Section: Introductionunclassified

Avaliação in vitro da atividade fotoprotetora de extratos vegetais do cerrado de Mato Grosso

Violante¹,

Souza²,

Venturini³

et al. 2009

Rev. bras. farmacogn.

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“…Non-irradiated controls for each concentration were kept in the dark during this period. After irradiation, sample and corresponding dark control slides were immediately transferred to lysis buffer for 40 min (to minimise DNA repair), followed by transfer to unwinding buffer (pH>13, incubated for 40 min) and electrophoresis (24 min at 20 V, 200 mA) according to the protocol described by Morley et al [53]. Slides were then placed in 100% ethanol for dehydration.…”

Section: Single Cell Gel Electrophoresismentioning

confidence: 99%

“…The following day cells were processed onto CometSlides (Trevigen, USA) using an alkali single cell gel electrophoresis protocol modified by Morley et al [53] and originally described by Singh et al [54]. Cells were then irradiated using the Aktilite 128 LED array at a distance of 5 cm and for a period of 3 min (15 J/cm 2 ; 635±2 nm; 75 mW/cm 2 ).…”

Section: Single Cell Gel Electrophoresismentioning

confidence: 99%

The importance of iron chelation and iron availability during PpIX-induced photodynamic therapy

Curnow

Pye

2015

Photonics &Amp; Lasers in Medicine

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“…1) [16]. An increasing body of literature evidence furthermore indicates that beneficial action of green tea drinking might be extended to a number of pathologies correlated to inflammation [4,5,11,15,17,20,22,24,26,27,35,38]. Since pathogenesis of inflammatory diseases seems not to be exclusively correlated to redox state unbalance, a theory that the beneficial effect of green tea drinking might not simply be achieved by its strong antioxidant properties is emerging.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of epigallocatechin-3-gallate on ischemia/reperfusion-induced injuries in the heart: STAT1 silencing flavonoid

et al. 2007

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The beneficial effect of naturally occurring flavonoids in health is believed to be due to their strong antioxidant activity. However, recent laboratory evidence indicates the involvement of a more specific action. Here, we present evidence that, among a number of catechins present in green tea extract, only epigallocatechin-3-gallate (EGCG) exerts a strong inhibitory action on interferon-celicited activation of signal transducer and activator of transcription 1 (STAT1). Protective action of EGCG in ischemia/reperfusion injury in the heart and the molecular mechanism of action, which has nothing to do with its antioxidant capacity are described.

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The green tea polyphenol (−)‐epigallocatechin gallate and green tea can protect human cellular DNA from ultraviolet and visible radiation‐induced damage

Abstract: The studies showed that green tea and/or some constituents can offer some protection against UV-induced DNA damage in human cell cultures and also in human peripheral blood samples taken post-tea ingestion.

Cited by 116 publications

References 25 publications

Avaliação in vitro da atividade fotoprotetora de extratos vegetais do cerrado de Mato Grosso

Avaliação in vitro da atividade fotoprotetora de extratos vegetais do cerrado de Mato Grosso

The importance of iron chelation and iron availability during PpIX-induced photodynamic therapy

Protective effect of epigallocatechin-3-gallate on ischemia/reperfusion-induced injuries in the heart: STAT1 silencing flavonoid

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