The Great Pond Snail (<i>Lymnaea stagnalis</i>) as a Model of Aging and Age-Related Memory Impairment: An Overview

Fodor, I; Svigruha, Réka; Kemenes, György; Kemenes, Ildikó; Pirger, Zsolt

doi:10.1093/gerona/glab014

Cited by 10 publications

(3 citation statements)

References 93 publications

(120 reference statements)

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“…Using our model system, the pond snail Lymnaea stagnalis , we have been able to begin to uncover aspects of the causal neuronal mechanisms underlying learning and its subsequent consolidation into long-term memory (LTM) [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

LPS-Induced Garcia Effect and Its Pharmacological Regulation Mediated by Acetylsalicylic Acid: Behavioral and Transcriptional Evidence

Rivi

Batabyal

Lukowiak

et al. 2023

Biology

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Lymnaea stagnalis learns and remembers to avoid certain foods when their ingestion is followed by sickness. This rapid, taste-specific, and long-lasting aversion—known as the Garcia effect—can be formed by exposing snails to a novel taste and 1 h later injecting them with lipopolysaccharide (LPS). However, the exposure of snails to acetylsalicylic acid (ASA) for 1 h before the LPS injection, prevents both the LPS-induced sickness state and the Garcia effect. Here, we investigated novel aspects of this unique form of conditioned taste aversion and its pharmacological regulation. We first explored the transcriptional effects in the snails’ central nervous system induced by the injection with LPS (25 mg), the exposure to ASA (900 nM), as well as their combined presentation in untrained snails. Then, we investigated the behavioral and molecular mechanisms underlying the LPS-induced Garcia effect and its pharmacological regulation by ASA. LPS injection, both alone and during the Garcia effect procedure, upregulated the expression levels of immune- and stress-related targets. This upregulation was prevented by pre-exposure to ASA. While LPS alone did not affect the expression levels of neuroplasticity genes, its combination with the conditioning procedure resulted in their significant upregulation and memory formation for the Garcia effect.

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Section: Introductionmentioning

confidence: 99%

LPS-Induced Garcia Effect and Its Pharmacological Regulation Mediated by Acetylsalicylic Acid: Behavioral and Transcriptional Evidence

Rivi

Batabyal

Lukowiak

et al. 2023

Biology

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“…Specifically, we found that amyloid-β peptides 1-42 and 25-35 were able to inhibit longterm memory recall in L. stagnalis (Ford et al, 2017;Ford et al, 2015). Recently, the sequencing of the whole transcriptome of the central nervous system (CNS) of L. stagnalis identified several evolutionarily conserved sequences of genes involved in human ageing, and age-related and amyloid-β-induced memory loss, including gelsolin, presenilin, huntingin, Parkinson disease protein 7 (PARK-7/DJ-1) and amyloid precursor protein (Fodor et al, 2021;Fodor et al, 2020b). However, the lack of comprehensive proteomics information in L. stagnalis has hindered further progress with research aimed at understanding the roles of these proteins in the context of large-scale protein networks in the CNS.…”

Section: Introductionmentioning

confidence: 99%

A combined bioinformatics and LC-MS-based approach for the development and benchmarking of a comprehensive database of Lymnaea CNS proteins

Wooller

Anagnostopoulou

Kuropka

et al. 2022

Journal of Experimental Biology

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Applications of key technologies in biomedical research, such as qRT-PCR or LC-MS based proteomics, are generating large biological (-omics) data sets which are useful for the identification and quantification of biomarkers in any research area of interest. Genome, transcriptome and proteome databases are already available for a number of model organisms including vertebrates and invertebrates. However, there is insufficient information available for protein sequences of certain invertebrates, such as the great pond snail Lymnaea stagnalis, a model organism that has been used highly successfully in elucidating evolutionarily conserved mechanisms of memory function and dysfunction. Here we used a bioinformatics approach to designing and benchmarking a comprehensive CNS proteomics database (LymCNS-PDB) for the identification of proteins from the CNS of Lymnaea by LC-MS based proteomics. LymCNS-PDB was created by using the Trinity TransDecoder bioinformatics tool to translate amino acid sequences from mRNA transcript assemblies obtained from a published Lymnaea transcriptomics database. The blast-style MMSeq2 software was used to match all translated sequences to UniProtKB sequences for molluscan proteins, including Lymnaea and other molluscs. LymCNS-PDB contains 9,628 identified matched proteins that were benchmarked by performing LC-MS based proteomics analysis with proteins isolated from the Lymnaea CNS. MS/MS analysis using the LymCNS-PDB database led to the identification of 3,810 proteins. Only 982 proteins were identified by using a non-specific molluscan database. LymCNS-PDB provides a valuable tool that will enable us to perform quantitative proteomics analysis of protein interactomes involved in several CNS functions in Lymnaea, including learning and memory and age-related memory decline.

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“…Lymnaea stagnalis , also known as the great pond snail, is an excellent model organism used for research in neuroscience, aging, ecotoxicology, parasite-host interactions, and evolution and development ( Jimenez et al, 2004 ; Hoek et al, 2005 ; Benjamin, 2008 ; Pirger et al, 2014 ; Fodor et al, 2020a , b , 2021 ). Specifically, the central nervous system (CNS) of L. stagnalis is highly characterized at multiple levels.…”

Section: Introductionmentioning

confidence: 99%

Neuropeptide Localization in Lymnaea stagnalis: From the Central Nervous System to Subcellular Compartments

Wood

Stopka

Zhang

et al. 2021

Front. Mol. Neurosci.

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Due to the relatively small number of neurons (few tens of thousands), the well-established multipurpose model organism Lymnaea stagnalis, great pond snail, has been extensively used to study the functioning of the nervous system. Unlike the more complex brains of higher organisms, L. stagnalis has a relatively simple central nervous system (CNS) with well-defined circuits (e.g., feeding, locomotion, learning, and memory) and identified individual neurons (e.g., cerebral giant cell, CGC), which generate behavioral patterns. Accumulating information from electrophysiological experiments maps the network of neuronal connections and the neuronal circuits responsible for basic life functions. Chemical signaling between synaptic-coupled neurons is underpinned by neurotransmitters and neuropeptides. This review looks at the rapidly expanding contributions of mass spectrometry (MS) to neuropeptide discovery and identification at different granularity of CNS organization. Abundances and distributions of neuropeptides in the whole CNS, eleven interconnected ganglia, neuronal clusters, single neurons, and subcellular compartments are captured by MS imaging and single cell analysis techniques. Combining neuropeptide expression and electrophysiological data, and aided by genomic and transcriptomic information, the molecular basis of CNS-controlled biological functions is increasingly revealed.

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The Great Pond Snail (Lymnaea stagnalis) as a Model of Aging and Age-Related Memory Impairment: An Overview

Cited by 10 publications

References 93 publications

LPS-Induced Garcia Effect and Its Pharmacological Regulation Mediated by Acetylsalicylic Acid: Behavioral and Transcriptional Evidence

LPS-Induced Garcia Effect and Its Pharmacological Regulation Mediated by Acetylsalicylic Acid: Behavioral and Transcriptional Evidence

A combined bioinformatics and LC-MS-based approach for the development and benchmarking of a comprehensive database of Lymnaea CNS proteins

Neuropeptide Localization in Lymnaea stagnalis: From the Central Nervous System to Subcellular Compartments

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