The grapevine homeobox gene VvHB58 influences seed and fruit development through multiple hormonal signaling pathways

Li, Yunduan; Zhang, Songlin; Dong, Ruzhuang; Wang, Li; Yao, Jin; Nocker, Steve van; Wang, Xiping

doi:10.1186/s12870-019-2144-9

Cited by 20 publications

(20 citation statements)

References 64 publications

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“…The three grapevine BBX coding regions without the termination codon were inserted into a pCambia2300-GFP vector driven by the CaMV 35S promoter. The resulting constructs were transformed into Agrobacterium tumefaciens GV3101 and infected into the leaves of Nicotiana benthamiana as previously described [ 68 ]. The transient expression of the fused proteins was observed by a laser scanning confocal microscope (Olympus FV3000, Japan) after 3 d at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification and expression analysis of the B-box transcription factor gene family in grapevine (Vitis vinifera L.)

Zhang

et al. 2021

BMC Genomics

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Background B-box (BBX) zinc-finger transcription factors play important roles in plant growth, development, and stress response. Although these proteins have been studied in model plants such as Arabidopsis thaliana or Oryza sativa, little is known about the evolutionary history or expression patterns of BBX proteins in grapevine (Vitis vinifera L.). Results We identified a total of 25 VviBBX genes in the grapevine genome and named them according to the homology with Arabidopsis. These proteins were classified into five groups on the basis of their phylogenetic relationships, number of B-box domains, and presence or absence of a CCT domain or VP motif. BBX proteins within the same group showed similar exon-intron structures and were unevenly distributed in grapevine chromosomes. Synteny analyses suggested that only segmental duplication events contributed to the expansion of the VviBBX gene family in grapevine. The observed syntenic relationships between some BBX genes from grapevine and Arabidopsis suggest that they evolved from a common ancestor. Transcriptional analyses showed that the grapevine BBX genes were regulated distinctly in response to powdery mildew infection and various phytohormones. Moreover, the expression levels of a subset of BBX genes in ovules were much higher in seedless grapevine cultivars compared with seeded cultivars during ovule development, implying a potential role in seed abortion. Additionally, VviBBX8, VquBBX15a and VquBBX29b were all located in the nucleus and had transcriptional activity except for VquBBX29b. Conclusions The results of this study establish the genome-wide analysis of the grapevine BBX family and provide a framework for understanding the biological roles of BBX genes in grapevine.

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Section: Methodsmentioning

confidence: 99%

Genome-wide identification and expression analysis of the B-box transcription factor gene family in grapevine (Vitis vinifera L.)

Zhang

et al. 2021

BMC Genomics

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“…Phytohormones, such as brassinosteroids (BRs), gibberellin (GA), auxin and cytokinin (CK) play important roles in seed development which directly control seed size by enhancing cell proliferation, affecting embryo and endosperm development (Jiang et al, 2013;Du et al, 2017;Hu et al, 2018;Assefa et al, 2019;Li Y. D. et al, 2019). Many genes regulate seed development through integration into plant hormone metabolism.…”

Section: Overall View Of the Genes And Regulatory Network Controllinmentioning

confidence: 99%

“…The rice gene qTGW3 encodes OsSK41 that interacts with OsARF4 (AUXIN RESPONSE FACTOR 4); loss-of-function mutant of OsSK41 has increased grain length and weight (Hu et al, 2018). The grape gene VvHB58 has a potential function in regulating seed number and development by impacting auxin, gibberellin, and ethylene signaling pathways (Li Y. D. et al, 2019). In soybean, Glyma.02g04660 encodes a histidine phosphotransfer protein ortholog that controls seed width through the cytokininmediated regulating pathway (Assefa et al, 2019).…”

Section: Overall View Of the Genes And Regulatory Network Controllinmentioning

confidence: 99%

Quantitative Trait Loci for Seed Size Variation in Cucurbits – A Review

et al. 2020

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Cucurbits (Cucurbitaceae family) include many economically important fruit vegetable crops such as watermelon, pumpkin/squash, cucumber, and melon. Seed size (SS) is an important trait in cucurbits breeding, which is controlled by quantitative trait loci (QTL). Recent advances have deciphered several signaling pathways underlying seed size variation in model plants such as Arabidopsis and rice, but little is known on the genetic basis of SS variation in cucurbits. Here we conducted literature review on seed size QTL identified in watermelon, pumpkin/squash, cucumber and melon, and inferred 14, 9 and 13 consensus SS QTL based on their physical positions in respective draft genomes. Among them, four from watermelon (ClSS2.2, ClSS6.1, ClSS6.2, and ClSS8.2), two from cucumber (CsSS4.1 and CsSS5.1), and one from melon (CmSS11.1) were majoreffect, stable QTL for seed size and weight. Whole genome sequence alignment revealed that these major-effect QTL were located in syntenic regions across different genomes suggesting possible structural and functional conservation of some important genes for seed size control in cucurbit crops. Annotation of genes in the four watermelon consensus SS QTL regions identified genes that are known to play important roles in seed size control including members of the zinc finger protein and the E3 ubiquitinprotein ligase families. The present work highlights the utility of comparative analysis in understanding the genetic basis of seed size variation, which may help future mapping and cloning of seed size QTL in cucurbits.

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“…Recently, the demand for seedless grapes is also increasing [23,24]. In recent years, scientists have reported genes such as VvYABBY4 and VvHB58 as having a role in grapevine fruit and seed development [25,26]. However, the key genes mediating this process still needs to be explored, such as, for example, GASA genes.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Characterization and Expression Profiling of GASA Genes during Different Stages of Seed Development in Grapevine (Vitis vinifera L.) Predict Their Involvement in Seed Development

Ahmad

Yao

Zhang

et al. 2020

IJMS

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Members of the plant-specific GASA (gibberellic acid-stimulated Arabidopsis) gene family have multiple potential roles in plant growth and development, particularly in flower induction and seed development. However, limited information is available about the functions of these genes in fruit plants, particularly in grapes. We identified 14 GASA genes in grapevine (Vitis vinifera L.) and performed comprehensive bioinformatics and expression analyses. In the bioinformatics analysis, the locations of genes on chromosomes, physiochemical properties of proteins, protein structure, and subcellular positions were described. We evaluated GASA proteins in terms of domain structure, exon-intron distribution, motif arrangements, promoter analysis, phylogenetic, and evolutionary history. According to the results, the GASA domain is conserved in all proteins and the proteins are divided into three well-conserved subgroups. Synteny analysis proposed that segmental and tandem duplication have played a role in the expansion of the GASA gene family in grapes, and duplicated gene pairs have negative selection pressure. Most of the proteins were predicted to be in the extracellular region, chloroplasts, and the vacuole. In silico promoter analysis suggested that the GASA genes may influence different hormone signaling pathways and stress-related mechanisms. Additionally, we performed a comparison of the expression between seedless (Thompson seedless) and seeded (Red globe) cultivars in different plant parts, including the ovule during different stages of development. Furthermore, some genes were differentially expressed in different tissues, signifying their role in grapevine growth and development. Several genes (VvGASA2 and 7) showed different expression levels in later phases of seed development in Red globe and Thompson seedless, suggesting their involvement in seed development. Our study presents the first genome-wide identification and expression profiling of grapevine GASA genes and provides the basis for functional characterization of GASA genes in grapes. We surmise that this information may provide new potential resources for the molecular breeding of grapes.

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The grapevine homeobox gene VvHB58 influences seed and fruit development through multiple hormonal signaling pathways

Cited by 20 publications

References 64 publications

Genome-wide identification and expression analysis of the B-box transcription factor gene family in grapevine (Vitis vinifera L.)

Genome-wide identification and expression analysis of the B-box transcription factor gene family in grapevine (Vitis vinifera L.)

Quantitative Trait Loci for Seed Size Variation in Cucurbits – A Review

Genome-Wide Characterization and Expression Profiling of GASA Genes during Different Stages of Seed Development in Grapevine (Vitis vinifera L.) Predict Their Involvement in Seed Development

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