The Granulation of Juxtaglomerular Cells in Renal Hypertension, Desoxycorticosterone and Post-Desoxycorticosterone Hypertension, Adrenal Regeneration Hypertension, and Adrenal Insufficiency12

The Effect of High Perfusion Pressures on the Granulation of Juxtaglomerular Cells in an Isolated Kidney*

“…Since adrenalectomy increases the granulation of the juxtaglomerular cells and desoxycorticosterone decreases it (1,3,5), the adrenals obviously affect juxtaglomerular granulation and it seemed important to determine whether an increased rate of secretion by intact adrenals was necessary to get the degranulation during perfusions at high pressure. Five rats were adrenalectomized and given cortisone acetate just before serving as the blood donors in the experiment.…”

“…The juxtaglomerular indices in both kidneys were estimated using the Hartroft method (3) as employed in our laboratory (1). Six different sections from each kidney were counted and then averaged, thereby increasing our precision two and one-half By determining the juxtaglomerular index of the isolated kidney at the end of the perfusion and by comparing it to the juxtaglomerular index of the control opposite kidney from the same rat, one can get a good estimate of the changes in juxtaglomerular granulation that occurred during the perfusion.…”

“…In our previous studies on the granulation of juxtaglomerular cells, we were impressed with several experiments in which a kidney was perfused with blood at a high pressure and had a striking decrease in the juxtaglomerular granulation (1). Conversely, in other situations where the pressure of the blood perfusing the kidney was reduced, the juxtaglomerular granulation tended to increase (1).…”

“…Conversely, in other situations where the pressure of the blood perfusing the kidney was reduced, the juxtaglomerular granulation tended to increase (1). These relationships can easily be indirect or coincidental, and it seemed of interest to find out whether changes in perfusion pressure can directly alter the granulation of these cells.…”

Effect of Angiotensin on Juxtaglomerular Cells and Vessels of the Kidney

“…Increased <-Increased angiotensin renin formation As one test of this concept, we determined to give rats repeated subcutaneous injections of pressor doses of angiotensin and to observe the effect on the juxtaglomerular (JG) cells, since Tobian et al 9 had previously shown that renal artery constriction in the rat produces an increase in the juxtaglomerular index (JGI).…”