The graft content of hyaluronan is increased during xenograft rejection

Lorant, Tomas; Tufveson, Gunnar; Johnsson, Cecilia

doi:10.1111/j.1399-3089.2004.00132.x

Cited by 7 publications

(9 citation statements)

References 31 publications

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“…According to our previous study,3 the acellular scaffold alone could actually increase HA production, although not reaching statistical significance. Furthermore, even though HA is often considered as antifibrotic and it is a key to the maintenance of optimal viscoelasticity of the vocal folds,8, 24 HA has also been shown to be associated with xenograft rejection 25. Based on these somewhat conflicting findings in the literature, our findings highlighted the need for further studies to elucidate the effect of the acellular scaffold on HA production as a function of HGF loading and release.…”

Section: Discussionmentioning

confidence: 82%

“…Furthermore, even though HA is often considered as antifibrotic and it is a key to the maintenance of optimal viscoelasticity of the vocal folds, 8,24 HA has also been shown to be associated with xenograft rejection. 25 Based on these somewhat conflicting findings in the literature, our findings highlighted the need for further studies to elucidate the effect of the acellular scaffold on HA production as a function of HGF loading and release. No difference in HA level was observed between the two groups after 30 days and 90 days.…”

Section: Discussionmentioning

confidence: 85%

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Controlled release of hepatocyte growth factor from a bovine acellular scaffold for vocal fold reconstruction

Chan

Weinberger

et al. 2009

J Biomedical Materials Res

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A bovine acellular scaffold was found to facilitate tissue remodeling in a rat model of vocal fold injury, whereas hepatocyte growth factor (HGF) has been shown to have an anti-scarring effect in the larynx. This study examined the loading and release kinetics of HGF in vitro, and the potential of the acellular scaffold as a timed-release system for the delivery of HGF in vivo. Bilateral wounds were created in the posterior vocal folds of 20 rats, with HGF-loaded acellular scaffolds implanted into the wounds unilaterally, and scaffolds without HGF implanted into the contralateral vocal folds as control. The rats were humanely sacrificed after 3, 7, 30, and 90 days and their larynges were examined histologically and immunohistochemically. Expressions of key matrix proteins in the vocal fold coronal sections were quantified by digital image analysis. Results demonstrated a gradual, sustained release of HGF for at least 7 days in vitro, consistent with the detection of glycosaminoglycans inherent of the scaffold. In rat vocal folds implanted with HGFloaded scaffolds, apparently fewer inflammatory cells were observed 3 days after surgery when compared to the control. The mean relative densities of collagen III and hyaluronic acid were significantly lower than those of the control 7 days after surgery. Scaffold implants were apparently degraded by 3 months in all animals, with no evidence of fibrosis or calcification. These data suggested that the bovine acellular scaffold could be promising for the exogenous delivery of select growth factors in vivo.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 85%

Controlled release of hepatocyte growth factor from a bovine acellular scaffold for vocal fold reconstruction

Chan

Weinberger

et al. 2009

J Biomedical Materials Res

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“…The content of hyaluronan during xenograft rejection was studied in a mouse‐to‐rat cardiac xenotransplantation model [19]. Hyaluronan, a macromolecule, which is associated with interstitial edema, was found to be significantly increased during xenograft rejection.…”

Section: Immunobiologymentioning

confidence: 99%

Xenotransplantation Literature Update May–June 2004

Bühler

2004

Xenotransplantation

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“…9 Allograft and xenograft rejection processes have been associated with a local accumulation of HA in the transplanted organs. [10][11][12][13] Furthermore, the significant increase of serum HA that parallels HA concentration within the allograft has been proposed as a potential marker of cardiac allograft rejection. 14 The graft and serum content of HA after transplantation includes both high-molecular-weight HA and fragments that result from endogenous hyaluronidase enzymatic activity and display specific functions depending on the size.…”

mentioning

confidence: 99%

“…14 The graft and serum content of HA after transplantation includes both high-molecular-weight HA and fragments that result from endogenous hyaluronidase enzymatic activity and display specific functions depending on the size. 10,11,15 Thus, 135-KDa fragments of HA induce dendritic cell maturation and initiation of alloimmunity by increasing the priming of allogeneic T cells. 11 Also, instilled HA-oligosaccharides of 25-75 KDa broke pre-established tolerance in a mouse model of lung allograft transplantation by directly activating innate immune signaling pathways.…”

mentioning

confidence: 99%

Antibodies against hyaluronan oligosaccharides in xenotransplantation

Bello-Gil

Olivera-Ardid

Tuzikov

et al. 2023

Xenotransplantation

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Carbohydrate‐specific antibodies are significant mediators of xenograft rejection. This study analyzed the carbohydrate specificity of antibodies in baboons before and after xenotransplantation of organs or injection of porcine red blood cells from hDAF transgenic pigs, using a glycan array with structurally defined glycans. Antibodies against hyaluronic acid disaccharide (HA2) showed the highest reactivity at baseline and rose after xenogeneic exposure. We also investigated in the serum of baboons that underwent xenotransplantation with either hDAF or hDAF/hMCP transgenic pig organs and Lewis rats after hamster‐skin xenotransplantation the specificity of anti‐HA antibodies on a glycan microarray representing HA oligosaccharides containing from two to 40 saccharides. Notably, the HA oligosaccharides ranging from 32 to 40 saccharides exhibited the highest antibody binding intensities at baseline in baboon and rat sera. After xenotransplantation, antibodies against HA38 and HA40 in baboons, and HA32, HA34, and HA36 in rats showed the highest titer increases. The changes of anti‐HA IgM and IgG antibodies in rats after skin xenotransplantation was also confirmed by an ELISA specific for HA2, HA24, and HA85 antibodies. Thus, xenotransplantation is associated with increased antibodies against HA‐oligosaccharides, which may represent a new target for intervention.

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The graft content of hyaluronan is increased during xenograft rejection

Abstract: These data suggest that the graft content of hyaluronan considerably increases during xenograft rejection. HAse effectively reduces this accumulation, but does not affect the average water content.

Cited by 7 publications

References 31 publications

Controlled release of hepatocyte growth factor from a bovine acellular scaffold for vocal fold reconstruction

Controlled release of hepatocyte growth factor from a bovine acellular scaffold for vocal fold reconstruction

Xenotransplantation Literature Update May–June 2004

Antibodies against hyaluronan oligosaccharides in xenotransplantation

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