The GPCR membrane receptor, DopEcR, mediates the actions of both dopamine and ecdysone to control sex pheromone perception in an insect

Abrieux, Antoine; Duportets, Line; Debernard, StÃ©phane; Gadenne, Christophe; Anton, Sylvia

doi:10.3389/fnbeh.2014.00312

Cited by 37 publications

(30 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lastly the delay of response following either pre-exposure, clothianidin treatment or the combination of the two was very short, indicating that the tuning and processing of the sex pheromone components within the AL occurred very rapidly for these treated males. A similar effect (high percentage and fast response) was obtained after dopamine treatments [45]. These results further confirm that behavioural responses of naive sexually mature A .…”

Section: Discussionsupporting

confidence: 84%

“…e . injections of JH mimetics or ecdysone [29, 30, 38], at the behavioural level by the application of dopamine [45] and at the neuronal level, without behavioural effect, by application of octopamine [36]. In addition to the effects on immature males, we show here that pre-exposure of sexually mature males still enhances their behavioural pheromone response, reaching very high levels (more than 80% responses).…”

Section: Discussionmentioning

confidence: 81%

See 1 more Smart Citation

An Insecticide Further Enhances Experience-Dependent Increased Behavioural Responses to Sex Pheromone in a Pest Insect

et al. 2016

Self Cite

View full text Add to dashboard Cite

Neonicotinoid insecticides are widely used to protect plants against pest insects, and insecticide residues remaining in the environment affect both target and non-target organisms. Whereas low doses of neonicotinoids have been shown to disturb the behaviour of pollinating insects, recent studies have revealed that a low dose of the neonicotinoid clothianidin can improve behavioural and neuronal sex pheromone responses in a pest insect, the male moth Agrotis ipsilon, and thus potentially improve reproduction. As male moth behaviour depends also on its physiological state and previous experience with sensory signals, we wondered if insecticide effects would be dependent on plasticity of olfactory-guided behaviour. We investigated, using wind tunnel experiments, whether a brief pre-exposure to the sex pheromone could enhance the behavioural response to this important signal in the moth A. ipsilon at different ages (sexually immature and mature males) and after different delays (2 h and 24 h), and if the insecticide clothianidin would interfere with age effects or the potential pre-exposure-effects. Brief pre-exposure to the pheromone induced an age-independent significant increase of sex pheromone responses 24 h later, whereas sex pheromone responses did not increase significantly 2 h after exposure. However, response delays were significantly shorter compared to naïve males already two hours after exposure. Oral treatment with clothianidin increased sex pheromone responses in sexually mature males, confirming previous results, but did not influence responses in young immature males. Males treated with clothianidin after pre-exposure at day 4 responded significantly more to the sex pheromone at day 5 than males treated with clothianidin only and than males pre-exposed only, revealing an additive effect of experience and the insecticide. Plasticity of sensory systems has thus to be taken into account when investigating the effects of sublethal doses of insecticides on behaviour.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 81%

An Insecticide Further Enhances Experience-Dependent Increased Behavioural Responses to Sex Pheromone in a Pest Insect

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is also possible that the modulation of AL neuron sensitivity is caused by increased dopamine release, which has been shown to be caused by clothianidin treatments in the rat brain [40]. Even though nothing is known about clothianidin effects on dopamine release in insects so far, the recently observed role of the dopamine/steroid receptor DopEcR in regulating the sensitivity of pheromone-responding AL neurons [28] would allow this type of modulation. Numerous studies have shown a negative effect of neonicotinoid insecticides on cognitive processes (i.e.…”

Section: Discussionmentioning

confidence: 99%

Low doses of a neonicotinoid insecticide modify pheromone response thresholds of central but not peripheral olfactory neurons in a pest insect

et al. 2016

Self Cite

View full text Add to dashboard Cite

Insect pest management relies mainly on neurotoxic insecticides, including neonicotinoids, leaving residues in the environment. There is now evidence that low doses of insecticides can have positive effects on pest insects by enhancing various life traits. Because pest insects often rely on sex pheromones for reproduction, and olfactory synaptic transmission is cholinergic, neonicotinoid residues could modify chemical communication. We recently showed that treatments with different sublethal doses of clothianidin could either enhance or decrease behavioural sex pheromone responses in the male moth, Agrotis ipsilon. We investigated now effects of the behaviourally active clothianidin doses on the sensitivity of the peripheral and central olfactory system. We show with extracellular recordings that both tested clothianidin doses do not influence pheromone responses in olfactory receptor neurons. Similarly, in vivo optical imaging does not reveal any changes in glomerular response intensities to the sex pheromone after clothianidin treatments. The sensitivity of intracellularly recorded antennal lobe output neurons, however, is upregulated by a lethal dose 20 times and downregulated by a dose 10 times lower than the lethal dose 0. This correlates with the changes of behavioural responses after clothianidin treatment and suggests the antennal lobe as neural substrate involved in clothianidin-induced behavioural changes.

show abstract

“…Several other genes that relate to reproduction in moths also have been determined by using RNAi, including the DopEcR that relates to sex pheromone response in Agrotis ipsilon [25,99], olfactory receptor in L. dispar [100] and S. litura [34], PBAN [27,36] and PBAN-R [101,102] in many moths that relate to the regulation of sex pheromone biosynthesis, and the FATP and GPAT in B. mori that relate to bombykol biosynthesis [103]. …”

Section: Use Of Rnai For Gene Function Analysis In Mothsmentioning

confidence: 99%

RNA Interference in Moths: Mechanisms, Applications, and Progress

Wang

Chen

et al. 2016

Genes

View full text Add to dashboard Cite

The vast majority of lepidopterans, about 90%, are moths. Some moths, particularly their caterpillars, are major agricultural and forestry pests in many parts of the world. However, some other members of moths, such as the silkworm Bombyx mori, are famous for their economic value. Fire et al. in 1998 initially found that exogenous double-stranded RNA (dsRNA) can silence the homolog endogenous mRNA in organisms, which is called RNA interference (RNAi). Soon after, the RNAi technique proved to be very promising not only in gene function determination but also in pest control. However, later studies demonstrate that performing RNAi in moths is not as straightforward as shown in other insect taxa. Nevertheless, since 2007, especially after 2010, an increasing number of reports have been published that describe successful RNAi experiments in different moth species either on gene function analysis or on pest management exploration. So far, more than 100 peer-reviewed papers have reported successful RNAi experiments in moths, covering 10 families and 25 species. By using classic and novel dsRNA delivery methods, these studies effectively silence the expression of various target genes and determine their function in larval development, reproduction, immunology, resistance against chemicals, and other biological processes. In addition, a number of laboratory and field trials have demonstrated that RNAi is also a potential strategy for moth pest management. In this review, therefore, we summarize and discuss the mechanisms and applications of the RNAi technique in moths by focusing on recent progresses.

show abstract

The GPCR membrane receptor, DopEcR, mediates the actions of both dopamine and ecdysone to control sex pheromone perception in an insect

Cited by 37 publications

References 48 publications

An Insecticide Further Enhances Experience-Dependent Increased Behavioural Responses to Sex Pheromone in a Pest Insect

An Insecticide Further Enhances Experience-Dependent Increased Behavioural Responses to Sex Pheromone in a Pest Insect

Low doses of a neonicotinoid insecticide modify pheromone response thresholds of central but not peripheral olfactory neurons in a pest insect

RNA Interference in Moths: Mechanisms, Applications, and Progress

Contact Info

Product

Resources

About