The Golgi complex

Sturgess, Jennifer M.; Katona, E.; Moscarello, Mario A.

doi:10.1007/bf01870012

Cited by 38 publications

(3 citation statements)

References 29 publications

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“…In the present study we have compared different kinetic parameters of two GalT enzymes. The origin of our Golgi GalT was ensured by the use of highly purified Golgi membranes (Sturgess et al, 1973) as starting material. The enzyme was purified by affinity chromatography with, as a ligand, a protein that is known to interact specifically with the GalT enzymes.…”

Section: Discussionmentioning

confidence: 99%

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A kinetic comparison of partially purified rat liver Golgi and rat serum galactosyltransferases

Paquet¹,

Moscarello²

1984

Biochemical Journal

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UDP-galactose: N-acetylglucosamine beta-1,4-galactosyltransferase was partially purified from rat liver Golgi membranes and rat serum. The kinetic parameters of the two enzymes isolated by affinity chromatography were compared with each other and with those for commercial bovine milk galactosyltransferase. When N-acetyl-glucosamine was the acceptor the Km values for UDP-galactose were 65,52 and 43 microM for the rat liver Golgi, rat serum and bovine milk enzymes respectively. The Km values for N-acetylglucosamine were 0.33, 1.49 and 0.5 mM for the three enzymes respectively. The Km values for UDP-galactose, with glucose as acceptor in the presence of 1 mg of alpha-lactalbumin, were 23, 9.0 and 60 microM for the three enzymes respectively, and the Km values for glucose were 2.3, 1.8 and 2.0 mM respectively. The effects of alpha-lactalbumin in both the lactosamine synthetase and lactose synthetase reactions were similar. The activation energies were 94.0 kJ/mol (22.5 kcal/mol) and 96.0 kJ/mol (22.9 kcal/mol) for the Golgi and serum enzymes respectively. Although some differences in Km values were observed between the rat liver Golgi and serum enzymes, the values obtained suggest a high degree of similarity between the kinetic properties of the three galactosyltransferases.

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Section: Discussionmentioning

confidence: 99%

“…The Golgi-enriched fraction was purified from male Wistar rats weighing 175 g by discontinuoussucrose-density-gradient centrifugation as previously described (Sturgess et al, 1973). The specific activity of GalT was used to monitor the purification.…”

Section: Purification Of Rat Liver Golgi Membranesmentioning

confidence: 99%

A kinetic comparison of partially purified rat liver Golgi and rat serum galactosyltransferases

Paquet¹,

Moscarello²

1984

Biochemical Journal

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“…Livers were immediately removed, placed into ice-cold 0.5 M sucrose containing 1 % dextran-250,37.5 mM Tris-maleate, 5 mM MgCl, and 3 mM mercaptoethanol, pH adjusted to 6.4. The Golgi fraction was isolated from the livers [13] and placed on another gradient, similar to the one reported earlier [14]. Basically, it consists of buffered sucrose layers at pH 6.4, with densities of 1.14, 1.16, 1.18 and 1.20 g/cm3.…”

Section: Methodsmentioning

confidence: 99%

Incorporation of Inorganic Sulfate in Rat-Liver Golgi

Katona

1976

Eur J Biochem

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Partial subcellular distribution and specifically the incorporation of 35S04 in the Golgi apparatus of rat liver was studied. The isolated Golgi fraction was placed on a further sucrose gradient for subfraction and in all samples the total incorporated label, the trichloroacetic-acid-soluble and insoluble, and chloroform/methanol-soluble and insoluble amounts of label were measured. Approximately 2.3 % of the total injected 35S was found in the liver, of which 2 was localized in the isolated Golgi fraction. Further subfractionation of the Golgi has shown that the majority of the 35S is localized at densities 1.12 -1.13 g/cm3 in the gradient. In the isolated Golgi fraction 85 of the label was present in the trichloroacetic-acid-soluble and 15 % in the insoluble part. Approximately 1 % of the label in the Golgi fraction was soluble in chloroform/methanol. On the bases of radioactivity per weight of protein and total radioactivity, the Golgi fractions had the highest activity, except the first supernatant which had higher radioactivity. Briefly, a significant amount of the 35S04 can be found in the isolated rat liver Golgi 15 min after the injection of the label, and the majority of this label is localized in the material sedimenting at density 1.12 -1.13 g/cm3 after subfractionation of the Golgi apparatus.

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Ultrastructure Methods in Cuticle Research

Locke¹,

Huie²

1980

Springer Series in Experimental Entomology

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The Golgi complex

Cited by 38 publications

References 29 publications

A kinetic comparison of partially purified rat liver Golgi and rat serum galactosyltransferases

A kinetic comparison of partially purified rat liver Golgi and rat serum galactosyltransferases

Incorporation of Inorganic Sulfate in Rat-Liver Golgi

Ultrastructure Methods in Cuticle Research

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