The Golgi Apparatus and Cell Plate Formation—a Postulate

Whaley, W. Gordon; Mollenhauer, Hilton H.

doi:10.1083/jcb.17.1.216

Cited by 179 publications

(39 citation statements)

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“…This depends on targeted secretion to generate a new plasma membrane (PM; Whaley and Mollenhauer, 1963;Samuels et al, 1995;Jurgens, 2005aJurgens, , 2005b. Plant cells control cytokinesis by constructing a plant-specific cytoskeletal configuration, the phragmoplast (Zhang et al, 1993;Granger and Cyr, 2000;Assaad, 2001).…”

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confidence: 99%

Vesicle Trafficking during Somatic Cytokinesis

2008

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Vesicle Trafficking during Somatic Cytokinesis

2008

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“…Bajer (1965a) assumed that small granules in the interzonal region and in the phragmoplast arose in situ, and that a difference existed between these small granules and the plate-forming droplets. In Zea and Spinacia, the array of small electron-dense vesicles, which appears prior to the large ones (Whaley and Mollenhauer 1963;Whaley et al 1966), may carry specific information related to microtubular disposition. Although Frey-Wyssling et al (1964), as well as some other investigators, have assumed that all vesicles in the cell plate region were dictyosome-derived, it may be that some of the vesicles, endowed with a nucleating function, are of different origin.…”

Section: Discussionmentioning

confidence: 99%

Effect of Digitonin on Cellular Division

Olah¹,

Hanzely

1973

CYTOLOGIA

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Optical and ultrastructuralinvestigations have shown that purified digitonin, a non-ionic, steroid-type saponin, dissociates the formation and function of the mitotic spindle from the phragmoplast (Olah 1963(Olah , 1965Underbrink and Olah 1965, 1968).The course of digitonin mitosis was described as similar to colchicine mitosis because it arrests the mitotic division at metaphase.However, during sub sequent phases of digitonin mitosis, a vigorously functioning phragmoplast develops among the paralyzed and scattered chromosomes, resulting in a branched or amor phously shaped cell plate and wall system which remains associated with the re stituted tetraploid nucleus.Our earlier studies using only KMnO4 fixation were extended to include glutaraldehyde-osmium tetroxide fixation, in order to clarify whether the effect of digitonin is related to the formation and function of the microtubules which constitute the mitotic spindle and the phragmoplast. Materials and methodsCloves of Allium sativum were rooted in distilled water. Rootlets, approxi mately 1cm long, were treated for 15 hours in 0.25% and 0.5% purified digitonin solutions. Appropriate controls, placed in distilled water, accompanied the treat ments. For electron microscopy, root tips were prefixed for 2 hours in 6% glutar aldehyde in 0.15M phosphate buffer (pH7.4) containing 3 drops of 0.1M CaCl2/ 10ml. The root tips were then rinsed for 30 minutes with the buffer and postfixed for 2 hours in 1% 0.15M phosphate buffered OsO4 at pH 7.4. Both fixations were carried out at room temperature. After 3-4 washes in distilled water, root tips were dehydrated in a graded methanol series, passed through propylene oxide, and embedded in Epon. Sections were cut with a diamond knife on a LKB ultra microtome, placed on uncoated grids, stained with uranyl acetate and lead citrate, and examined with a RCA EMU-3C or Hitachi HU-11A electron microscope. ResultsIn untreated cells, the microtubules showed their characteristic cortical,

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“…All the components of this cytoplasmic appara- (5), and Whaley and Mollenhauer (12) suggest that, like the Golgi complex in animals, dictyosomes in plants play a role in cellular secretion. It is thus of particular interest to find in an achlorophyllous plant tissue, known to have secretory activity (@ 7, 8, 10), an additional membrane system resembling the Golgi complex.…”

Section: Observations and Discussionmentioning

confidence: 99%