The Glyphosate-Based Herbicide Roundup Does Not Elevate Genome-Wide Mutagenesis of<i>Escherichia coli</i>

Tincher, Clayton; Behringer, Megan G; Walker, Noah; Lynch, Michael

doi:10.1534/g3.117.300133

Cited by 16 publications

(13 citation statements)

References 30 publications

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“…Screening AMR profiles in soil microbiota from organic and non-organic farming showed genes for resistance to clinical antibiotics in both types of soils; the antimicrobial resistance of the cultivable isolates, including clinically relevant ones, was mediated by efflux pumps ( 8 ). Nevertheless, considering that sub-lethal chronic exposure of E.coli to glyphosate-based herbicides is not mutagenic ( 9 ), epigenetic effects of such pesticides are still likely.…”

Section: Pesticides As Triggers: Resistance Developed Beyond the Clinmentioning

confidence: 99%

From environment to clinic: the role of pesticides in antimicrobial resistance

Malagón-Rojas

Parra-Barrera

Lagos

2020

Revista Panamericana De Salud Pública

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Antimicrobial resistance (AMR) in pathogens has been associated mainly with excessive use of antibiotics. Most studies of resistance have focused on clinical pathogens; however, microorganisms are exposed to numerous anthropogenic substances. Few studies have sought to determine the effects of chemical substances on microorganisms. Exposure to these substances may contribute to increased rates of AMR. Understanding microorganism communities in natural environments and AMR mechanisms under the effects of anthropogenic substances, such as pesticides, is important to addressing the current crisis of antimicrobial resistance. This report draws attention to molecules, rather than antibiotics, that are commonly used in agrochemicals and may be involved in developing AMR in non-clinical environments, such as soil. This report examines pesticides as mediators for the appearance of AMR, and as a route for antibiotic resistance genes and antimicrobial resistant bacteria to the anthropic environment. Available evidence suggests that the natural environment may be a key dissemination route for antibiotic-resistant genes. Understanding the interrelationship of soil, water, and pesticides is fundamental to raising awareness of the need for environmental monitoring programs and overcoming the current crisis of AMR.

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Section: Pesticides As Triggers: Resistance Developed Beyond the Clinmentioning

confidence: 99%

From environment to clinic: the role of pesticides in antimicrobial resistance

Malagón-Rojas

Parra-Barrera

Lagos

2020

Revista Panamericana De Salud Pública

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“…First, we identified 17 lab evolution projects with published (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43) information about the experimental design, 4 of which were MA studies (40)(41)(42)(43). For each study, we counted the number of non-synonymous mutations in TFs and TGs relative to the number of corresponding sites.…”

Section: A B C Dmentioning

confidence: 99%

Dynamics of genetic variation in Transcription Factors and its implications for the evolution of regulatory networks in Bacteria

Ali

Seshasayee

2019

Preprint

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The evolution of bacterial regulatory networks has largely been explained at macroevolutionary scales through lateral gene transfer and gene duplication. Transcription factors (TF) have been found to be less conserved across species than their target genes (TG). This would be expected if TFs accumulate mutations faster than TGs. This hypothesis is supported by several lab evolution studies which found TFs, especially global regulators, to be frequently mutated. Despite these studies, the contribution of point mutations in TFs to the evolution of regulatory network is poorly understood. We tested if TFs show greater genetic variation than their TGs using whole-genome sequencing data from a large collection of E coli isolates. We found TFs to be less diverse, across natural isolates, due to their regulatory roles. TFs were enriched in mutations in multiple adaptive lab evolution studies but not in mutation accumulation. However, over long-term evolution, relative frequency of mutations in TFs showed a gradual decay after a rapid initial burst. Our results suggest that point mutations, conferring large-scale expression changes, may drive the early stages of adaptation but gene regulation is subjected to stronger purifying selection post adaptation.

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“…As a soil bacterium B. subtilis can get into contact with growth-inhibiting substances like antibiotics and herbicides, which may be of anthropogenic origin. Moreover, due to the fact that glyphosate is toxicologically safe and that transgenic, glyphosate-resistant crops have been introduced, the herbicide has become the dominant 'Weedkiller' worldwide (Li and Long, 1988;Duke and Powles, 2008;Arjó et al, 2013;Benbrook, 2016;Mesnage and Antoniou, 2017;Tincher et al, 2017;see below). Glyphosate is resistant to chemical hydrolysis, thermal decomposition and photolysis due to a stable C-P bond (Kononova and Nesmeyanova, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Glyphosate is resistant to chemical hydrolysis, thermal decomposition and photolysis due to a stable C-P bond (Kononova and Nesmeyanova, 2002). Moreover, due to the fact that glyphosate is toxicologically safe and that transgenic, glyphosate-resistant crops have been introduced, the herbicide has become the dominant 'Weedkiller' worldwide (Li and Long, 1988;Duke and Powles, 2008;Arjó et al, 2013;Benbrook, 2016;Mesnage and Antoniou, 2017;Tincher et al, 2017;see below).…”

Section: Introductionmentioning

confidence: 99%

Identification of the first glyphosate transporter by genomic adaptation

Wicke

Schulz

Lentes

et al. 2019

Environmental Microbiology

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Summary The soil bacterium Bacillus subtilis can get into contact with growth‐inhibiting substances, which may be of anthropogenic origin. Glyphosate is such a substance serving as a nonselective herbicide. Glyphosate specifically inhibits the 5‐enolpyruvyl‐shikimate‐3‐phosphate (EPSP) synthase, which generates an essential precursor for de novo synthesis of aromatic amino acids in plants, fungi, bacteria and archaea. Inhibition of the EPSP synthase by glyphosate results in depletion of the cellular levels of aromatic amino acids unless the environment provides them. Here, we have assessed the potential of B. subtilis to adapt to glyphosate at the genome level. In contrast to Escherichia coli, which evolves glyphosate resistance by elevating the production and decreasing the glyphosate sensitivity of the EPSP synthase, B. subtilis primarily inactivates the gltT gene encoding the high‐affinity glutamate/aspartate symporter GltT. Further adaptation of the gltT mutants to glyphosate led to the inactivation of the gltP gene encoding the glutamate transporter GltP. Metabolome analyses confirmed that GltT is the major entryway of glyphosate into B. subtilis. GltP, the GltT homologue of E. coli also transports glyphosate into B. subtilis. Finally, we found that GltT is involved in uptake of the herbicide glufosinate, which inhibits the glutamine synthetase.

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The Glyphosate-Based Herbicide Roundup Does Not Elevate Genome-Wide Mutagenesis ofEscherichia coli

Cited by 16 publications

References 30 publications

From environment to clinic: the role of pesticides in antimicrobial resistance

From environment to clinic: the role of pesticides in antimicrobial resistance

Dynamics of genetic variation in Transcription Factors and its implications for the evolution of regulatory networks in Bacteria

Identification of the first glyphosate transporter by genomic adaptation

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