The glyoxylate cycle is required for temporal regulation of virulence by the plant pathogenic fungus<i>Magnaporthe grisea</i>

Wang, Zhengyi; Thornton, Christopher R.; Kershaw, Michael J.; Li, Debao; Talbot, Nicholas J.

doi:10.1046/j.1365-2958.2003.03412.x

Cited by 388 publications

(228 citation statements)

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“…Growth on stored carbon sources is also important during various developmental stages and for the provision of substrates in the synthesis of secondary metabolites. The utilization of carbon sources during infection by fungal pathogens may have profound effects on pathogenicity (e.g., Thines et al 2000;Lorenz and Fink 2002;Wang et al 2003;Barelle et al 2006).…”

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confidence: 99%

Transcriptional Control of Gluconeogenesis in Aspergillus nidulans

et al. 2007

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Aspergillus nidulans can utilize carbon sources that result in the production of TCA cycle intermediates, thereby requiring gluconeogenesis. We have cloned the acuG gene encoding fructose-1,6 bisphosphatase and found that expression of this gene is regulated by carbon catabolite repression as well as by induction by a TCA cycle intermediate similar to the induction of the previously studied acuF gene encoding phosphoenolpyruvate carboxykinase. The acuN356 mutation results in loss of growth on gluconeogenic carbon sources. Cloning of acuN has shown that it encodes enolase, an enzyme involved in both glycolysis and gluconeogenesis. The acuN356 mutation is a translocation with a breakpoint in the 59 untranslated region resulting in loss of expression in response to gluconeogenic but not glycolytic carbon sources. Mutations in the acuK and acuM genes affect growth on carbon sources requiring gluconeogenesis and result in loss of induction of the acuF, acuN, and acuG genes by sources of TCA cycle intermediates. Isolation and sequencing of these genes has shown that they encode proteins with similar but distinct Zn(2) Cys(6) DNA-binding domains, suggesting a direct role in transcriptional control of gluconeogenic genes. These genes are conserved in other filamentous ascomycetes, indicating their significance for the regulation of carbon source utilization.

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confidence: 99%

Transcriptional Control of Gluconeogenesis in Aspergillus nidulans

et al. 2007

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“…Accumulation of high concentration glycerol in appressorium was considered to be the main cause of turgor (de Jong et al, 1997). Glycerol is a degradation product of triacyglycerol, and can also come from gluconeogenesis demonstrated by a mutant research of gene ICL1 encoding isocitrate lyase in M. grisea (Thines et al, 2000;Wang et al, 2003). We also found a homologue of pyruvate carboxylase involved in gluconeogenesis up-regulated in mature appressoria.…”

Section: Metabolism Changes Related To Appressorium Turgormentioning

confidence: 55%

Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea

Jin

Dong

You-liang

et al. 2007

J. Zhejiang Univ. - Sci. B

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Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene expression profiles of appressorium development provides insight into the molecular basis of pathogenicity and control of this fungal plant disease. A cDNA array representing 2927 unique genes based on a large EST (expressed sequence tag) database of M. grisea strain Y34 was constructed and used to profile the gene expression patterns at mycelium and appressorium maturation stages. Compared with mycelia, 55 up-regulated and 22 down-regulated genes were identified in mature appressoria. Among 77 genes, 16 genes showed no similarity to the genome sequences of M. grisea. A novel homologue of peptidyl-prolyl cis-trans isomerase was found to be expressed at low-level in mature appressoria of M. grisea. The results indicated that the genes such as pyruvate carboxylase, phospholipid metabolism-related protein and glyceraldehyde 3-phosphate dehydrogenase involved in gluconeogenesis, lipid metabolism and glycolysis, showed differential expression in mature appressoria. Furthermore, genes such as PTH11, beta subunit of G protein and SGT1 involved in cell signalling, were expressed differentially in mature appressoria. Northern blot analysis was used to confirm the cDNA array results.

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“…The glyoxylate cycle plays an important role in the nutrition of both plant-and animal-pathogenic fungi. These include plant pathogens Magnaporthe grisea (88), Leptosphaeria maculans (42), and Stagonospora nodorum (76) and animal pathogens such as C. albicans (51) and Aspergillus fumigatus (27). Fungal mutants with mutations in the glyoxylate cycle enzymes isocitrate lyase or malate synthase have low levels of germination and are not infectious.…”

Section: Developmental Regulation Of Pathogenesismentioning

confidence: 99%