The glutathione S-transferase (PxGST2L) may contribute to the detoxification metabolism of chlorantraniliprole in Plutella xylostella(L.)

Abstract

The diamondback moth (Plutella xylostella L.), is an economic pest of cruciferous plants worldwide, which causes great economic loss to cruciferous plants production. However, the pest has developed resistance to insecticides. One of such insecticides is chlorantraniliprole. The study of the mechanisms underlying resistance is key for the effective management of resistance. In this study, a comparative proteomics approach was used to isolate and identify various proteins that differed between chlorantraniliprole-susceptible and -resistant strains of P. xylostella. Eleven proteins were significantly different and were successfully identified by MALDI-TOF-MS. Metabolism-related proteins accounted for the highest proportion among the eleven different proteins. The function of the PxGST2L protein was validated by RNAi. Knockdown of PxGST2L reduced the GST activity and increased the toxicity of chlorantraniliprole to the diamondback moth. The resistance ratio of diamondback moth to chlorantraniliprole was reduced from 1029 to 505. The results indicated that PxGST2L is partly responsible for chlorantraniliprole insecticide resistance in DBM. Our finding contributes to the understanding of the mechanism underlying resistance to chlorantraniliprole in the DBM, to develop effective resistance management tactics.

Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

“…For examples, CpGSTd1 expressed higher in fat body and silk gland of C. pomonella , CsGSTe4 and CsGSTu1 are primarily expressed in fat body and midgut of C. suppressalis (Liu et al, 2014; Meng et al, 2022). Using RNAi combined with bioassay, a lot of GST genes have been demonstrated to involve in the detoxification metabolism and susceptibility of insecticides, such as CpGSTd1 in lambda‐cyhalothrin detoxification of C. pomonella (Liu et al, 2014), DcGSTe2 in the detoxification metabolism of fenpropathrin in Diaphorina citri (Yu & Killiny, 2018), the PxGST2L in chlorantraniliprole‐resistant P. xylostella (Yin et al, 2021) and CsGSTe4 and CsGSTu1 in the abamectin‐resistant of C. suppressalis (Liu et al, 2014; Meng et al, 2022). The sensibility of G. molesta larvae was obviously increased when knockdown of GmGSTo2 , GmGSTs1 and GmGSTz1 after exposure with EB, CT and LC compared with the control.…”

Insecticide Resistance Monitoring in Field Populations of the Whitebacked Planthopper Sogatella furcifera (Horvath) in China, 2019–2020

“…Enzyme activities can play a key role in understanding the patterns of resistance and insect susceptibility to chemicals [38]. Many previous reports found that P450, EST and GST were commonly the primary detoxification enzymes that help insect pests degrade a different type of xenobiotic [39][40][41]. Our results indicated that significant differences existed in the activity of detoxification enzymes between different populations.…”

Toxicological and transcriptomic effects in Mythimna separata (Lepidoptera: Noctuidae) exposed to chlorantraniliprole and functional characterization of glutathione S‐transferases

“…Studies have shown that CAP resistance is related to the expression level or activity of GSTs. Knockdown of PxGST2L increased the toxicity of CAP to the diamondback moth 60 . There is a strong correlation between GST enzyme activity and resistance to CAP in P. xylostella 61 .…”

“…Knockdown of PxGST2L increased the toxicity of CAP to the diamondback moth. 60 There is a strong correlation between GST enzyme activity and resistance to CAP in P. xylostella. 61 CAP induced the mRNA level of a sigma class GST gene in the Asiatic rice borer, Chilo suppressalis.…”