The glucose‐induced polyphosphoinositides turnover in <i>Saccharomyces cerevisiae</i> is not dependent on the CDC25‐RAS mediated signal transduction pathway

Frascotti, Gianni; Baroni, Debora; Martegani, Enzo

doi:10.1016/0014-5793(90)81319-j

Cited by 22 publications

References 16 publications

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3‐Nitrocoumarin is an efficient inhibitor of budding yeast phospholipase‐C

Tisi

Coccetti

Banfi

et al. 2001

Cell Biochemistry & Function

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3-Nitrocoumarin is described in the literature as a specific inhibitor of mammalian phospholipase-C and here we studied the effect of 3-nitrocoumarin on budding yeast phosphatidylinositol-specific phospholipase-C and its effect on yeast growth. 3-Nitrocoumarin is a powerful inhibitor in vitro of the yeast Plc1 protein with an IC(50) of 57 nM and it is also an inhibitor of yeast growth in minimal media at comparable concentrations. Moreover at the same concentration it inhibits the glucose-induced PI-turnover. Since the effects of 3-nitrocoumarin on yeast growth are superimposable on the growth phenotype caused by PLC1 gene deletion we can conclude that 3-nitrocoumarin is a specific and selective inhibitor of yeast phospholipase-C. In addition we show that 3-nitrocoumarin was also an effective inhibitor of the pathogenic yeast Candida albicans.

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3‐Nitrocoumarin is an efficient inhibitor of budding yeast phospholipase‐C

Tisi

Coccetti

Banfi

et al. 2001

Cell Biochemistry & Function

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Anthony H. Rose Memorial Lecture: The Ins and Outs of the Yeast Plasma Membrane*

Wheals

1996

Journal of the Institute of Brewing

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The last three pieces of research associated with the late Anthony H. Rose, Professor of Microbiology at the University of Bath, are reviewed. The second messenger cyclic AMP was shown to vary up to 70-fold in concentration during the growth of batch cultures of Saccharomyces cereviaiaa with different media. It seems to have a role in transducing information on actual levels of extracellular residual carbon source as well as entry into stationary phase. Another second messenger pathway, involving hydrolysis of phosphatidylinositol bisphosphate, was shown not to be glucose-stimulated as previously thought. Inositol trisphosphate kinase activity was detected but both the existence of the complete pathway and its role were unclear. The yeast Kluyveromyces marxianua isolated from cocoa fermentations was shown to produce abundant and almost pure endo-polygalacturonase in a constitutive manner. Some strains of Saccharomycas cereviafae also secrete this pectinolytic enzyme. All three projects relate to the central role of membranes in regulating transport of either sensory information or molecules in and out of cells, a key focus of his research for thirty years.

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