The Glossina morsitans tsetse fly saliva: General characteristics and identification of novel salivary proteins

Abbeele, Jan Van Den; Caljon, Guy; Dierick, Jean‐François; Moens, Lotte; Ridder, Karin De; Coosemans, M.

doi:10.1016/j.ibmb.2007.06.006

Cited by 32 publications

(47 citation statements)

References 36 publications

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“…Lipocalins expanded in assassin bugs and ticks [8,[23][24][25][26][51][52][53][54][55][56][57][58][59][60][61][62][63][64] . Tsetse flies show a predominance of the endonuclease family [27,28] . Expansion of the acid phosphatase and FS1 antigen families occurred in fleas [21] .…”

Section: The Same Protein Families Are Prone To Expansion In Closely mentioning

confidence: 99%

Evolution of Vertebrate Hemostatic and Inflammatory Control Mechanisms in Blood-Feeding Arthropods

Mans¹

2010

J Innate Immun

100

103

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Arthropods evolved blood-feeding lifestyles on more than 20 independent occasions. A comparison of sialomes (secretory salivary gland protein family repertoires) corroborates this and indicates that unique protein families are found for different blood-feeding lineages. Closely related lineages considered to have evolved blood-feeding behavior independently might share similar sets of protein families in their sialomes. No orthologous relationships or conserved functional mechanisms have, however, been found for these apparently related protein family members. This implies that sialomes were already defined before the divergence of these related lineages, but that the functions involved in blood feeding evolved at a later stage. Protein families prone to lineage-specific expansion (gene duplication) tend to be abundant with specialized functions associated with various gene duplicates. A central theme that seems to emerge from comparative sialomic analysis is that of the convergent evolution of modulatory strategies that targets host defenses even if the molecular mechanisms differ.

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Section: The Same Protein Families Are Prone To Expansion In Closely mentioning

confidence: 99%

Evolution of Vertebrate Hemostatic and Inflammatory Control Mechanisms in Blood-Feeding Arthropods

Mans¹

2010

J Innate Immun

100

103

View full text Add to dashboard Cite

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“…Tsetse flies (G. m. morsitans) were maintained at the Institute of Tropical Medicine Antwerp as described elsewhere (Van Den Abbeele et al, 2007). Experiments were approved by VUB and ITM animal ethics committees.…”

Section: Animalsmentioning

confidence: 99%

“…Full-length cDNA sequences encoding TAg5, previously described by Li et al (2001), were identified from a lgt11 salivary gland expression library (Van Den Abbeele et al, 2007). Signal peptide cleavage site prediction was performed using the SignalP 3.0 server.…”

Section: Cdna Sequence Analysis and Structure Predictionmentioning

confidence: 99%

“…The tag5 gene including the secretion signal sequence was amplified from a salivary gland cDNA library (Van Den Abbeele et al, 2007) by PCR (Mastercycler personal, Eppendorf) using the TAg5Forward (5 0 -CGCGGATCCACCATGAATTTCGTGCTTGCGACCTTAA GC-3 0 ) and TAg5Reverse (5 0 -TTAATGATATTCGACACCATTTTTGATCC ATTTATTGACC-3 0 ) primers. Following TA-cloning in a pGEM-T vector (Promega), the tag5 gene was integrated in a pVL1393 vector for the expression in S. frugiperda (Sf9) insect cells (Invitrogen) using the NcoI and BamHI restriction enzymes (NEB).…”

Section: Recombinant Tag5 Expression In Spodoptera Frugiperda Cellsmentioning

confidence: 99%

“…A cDNA, Tsetse Antigen5 (tag5), that corresponds to the 28 kDa IgE-binding protein, was picked up during our immune screening of a G. m. morsitans lgt11 salivary gland expression library (Van Den Abbeele et al, 2007). The full-length tag5 cDNA encodes a protein of 259 amino acids with 13 cysteine residues and a predicted molecular weight of 28,925 Da.…”

Section: Cdna Analysis Of the Putative 28 Kda Allergenmentioning

confidence: 99%

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