The Global Prevalence of Daptomycin, Tigecycline, and Linezolid-Resistant Enterococcus faecalis and Enterococcus faecium Strains From Human Clinical Samples: A Systematic Review and Meta-Analysis

Dadashi, Masoud; Sharifian, Parastoo; Bostanshirin, Nazila; Hajikhani, Bahareh; Bostanghadiri, Narjess; Khosravi-Dehaghi, Nafiseh; Belkum, Alex van; Darban-Sarokhalil, Davood

doi:10.3389/fmed.2021.720647

Cited by 34 publications

(32 citation statements)

References 59 publications

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“…In the present investigation, 34.95% of isolates sensitive to tigecycline were found, even though usually higher percentages of susceptible strains have been detected both in humans and domestic animals [ 42 , 43 ]. The tet genes were found to be equally distributed among susceptible, intermediate, and resistant isolates, confirming that these genes are not involved in tigecycline resistance [ 31 ].…”

Section: Discussionmentioning

confidence: 75%

Antimicrobial-Resistant Enterococcus spp. in Wild Avifauna from Central Italy

et al. 2022

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Bacteria of the genus Enterococcus are opportunistic pathogens, part of the normal intestinal microflora of animals, able to acquire and transfer antimicrobial resistance genes. The aim of this study was to evaluate the possible role of wild avifauna as a source of antimicrobial-resistant enterococci. To assess this purpose, 103 Enterococcus spp. strains were isolated from the feces of wild birds of different species; they were tested for antimicrobial resistance against 21 molecules, vancomycin resistance, and high-level aminoglycosides resistance (HLAR). Furthermore, genes responsible for vancomycin, tetracycline, and HLAR were searched. E. faecium was the most frequently detected species (60.20% of isolates), followed by E. faecalis (34.95% of isolates). Overall, 99.02% of the isolated enterococci were classified as multidrug-resistant, with 19.41% extensively drug-resistant, and 2.91% possible pan drug-resistant strains. Most of the isolates were susceptible to amoxicillin/clavulanic acid (77.67%) and ampicillin (75.73%), with only 5.83% of isolates showing an ampicillin MIC ≥ 64 mg/L. HLAR was detected in 35.92% of isolates, mainly associated with the genes ant(6)-Ia and aac(6′)-Ie-aph(2′’)-Ia. Few strains (4.85%) were resistant to vancomycin, and the genes vanA and vanB were not detected. A percentage of 54.37% of isolates showed resistance to tetracycline; tet(M) was the most frequently detected gene in these strains. Wild birds may contribute to the spreading of antimicrobial-resistant enterococci, which can affect other animals and humans. Constant monitoring is essential to face up to the evolving antimicrobial resistance issue, and monitoring programs should include wild avifauna, too.

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Section: Discussionmentioning

confidence: 75%

Antimicrobial-Resistant Enterococcus spp. in Wild Avifauna from Central Italy

et al. 2022

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“…Enterococcus faecalis is normally found in the human gastrointestinal tract and is often found associated with laboratory Drosophila (Cox and Gilmore, 2007;Broderick and Lemaitre, 2012;Loch et al, 2017) (this study). Of the five species described above that were cultured from mated females, Enterococcus faecalis was chosen for further study because of its ease of culture, frequent association with Drosophila, and its relevance to human health (Kao and Kline, 2019;Dadashi et al, 2021). To determine whether Enterococcus faecalis might affect life span and interact with mifepristone in vivo, adult females were assayed for life span using control media and media supplemented with Enterococcus faecalis in the presence or absence of mifepristone.…”

Section: Supplementation Of Media With Enterococcus Faecalis Has No E...mentioning

confidence: 99%

Mifepristone Increases Life Span in Female Drosophila Without Detectable Antibacterial Activity

et al. 2022

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Mifepristone dramatically increases the life span of mated female Drosophila while reducing the expression of innate immune response genes. Previous results indicated that mifepristone also reduced the load of aero-tolerant bacteria in mated females. Experiments were conducted to further investigate the possible role of bacteria in mifepristone life span effects. Life span was assayed in flies grown from sterilized eggs on autoclaved media and in normally cultured controls in two independent assays. Sterilization increased mated female life span (+8.3% and +57%, respectively), and the effect of mifepristone was additive (+53% and +93%, respectively). High-throughput sequencing of 16S sequences revealed that sterilization reduced the abundance of multiple species and the classes Bacteroidia, Bacilli, Actinobacteria, and Cytophagia. By contrast, mifepristone caused no decreases and instead increased the abundance of three species. Five aero-tolerant bacterial species were cultured from extracts of mated female flies, including both Gram-positive and Gram-negative species (Acetobacter sicerae, Enterococcus faecalis, Lactobacillus plantarum, Serratia rubidea, and Paenibacillus glucanolyticus). There was no detectable effect of mifepristone on the growth of these bacteria in vitro, indicating that mifepristone does not have a direct antibiotic effect. To test if antibiotics could mimic the effects of mifepristone in vivo, mated female flies were treated throughout adult life span with high concentrations of the individual antibiotics doxycycline, ampicillin, kanamycin, and streptomycin, in replicate experiments. No significant effect on life span was observed for ampicillin, kanamycin, or streptomycin, and an inconsistent benefit was observed for doxycycline. Finally, supplementation of media with Enterococcus faecalis did not alter adult female life span in the presence or absence of mifepristone. Taken together, the results indicate the life span benefits of mifepristone are not due to an antibiotic effect.

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“…In 2021, Dadashi et al reported that the global prevalence of E. faecium among enterococcal isolates from clinical infections was 40.6%, with 43.6 % in Asia, 38.0 % in Europe, and 36.8 % in America [21].…”

Section: Introductionmentioning

confidence: 99%

Exploring the mobilome and resistome of Enterococcus faecium in a One Health context across two continents

et al. 2022

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Enterococcus faecium is a ubiquitous opportunistic pathogen that is exhibiting increasing levels of antimicrobial resistance (AMR). Many of the genes that confer resistance and pathogenic functions are localized on mobile genetic elements (MGEs), which facilitate their transfer between lineages. Here, features including resistance determinants, virulence factors and MGEs were profiled in a set of 1273 E. faecium genomes from two disparate geographic locations (in the UK and Canada) from a range of agricultural, clinical and associated habitats. Neither lineages of E. faecium , type A and B, nor MGEs are constrained by geographic proximity, but our results show evidence of a strong association of many profiled genes and MGEs with habitat. Many features were associated with a group of clinical and municipal wastewater genomes that are likely forming a new human-associated ecotype within type A. The evolutionary dynamics of E. faecium make it a highly versatile emerging pathogen, and its ability to acquire, transmit and lose features presents a high risk for the emergence of new pathogenic variants and novel resistance combinations. This study provides a workflow for MGE-centric surveillance of AMR in Enterococcus that can be adapted to other pathogens.

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The Global Prevalence of Daptomycin, Tigecycline, and Linezolid-Resistant Enterococcus faecalis and Enterococcus faecium Strains From Human Clinical Samples: A Systematic Review and Meta-Analysis

Cited by 34 publications

References 59 publications

Antimicrobial-Resistant Enterococcus spp. in Wild Avifauna from Central Italy

Antimicrobial-Resistant Enterococcus spp. in Wild Avifauna from Central Italy

Mifepristone Increases Life Span in Female Drosophila Without Detectable Antibacterial Activity

Exploring the mobilome and resistome of Enterococcus faecium in a One Health context across two continents

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