The Gingival Crevicular Fluid Levels of IL-1β, IL-6 and TNF-α in Late Adult Rats

Abstract

Aim: To evaluate the levels of interleukin1β (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) in the samples of gingival crevicular fluid (GCF) taken from the late adult rats during the orthodontic tooth movement and to evaluate the responses to orthodontic treatment . Methodology: In experiment 19 adult (120 days) Spraque-Dawley rats were used. Approximately 15 g force applying open coil spring was applied actively between the upper incisors of the rats. Before and after the activation on the 3rd and 7th and 10th days GCF samples were taken from the vestibular surfaces of appliance fixed teeth using periopaper®. Then the samples were biochemically analyzed. For the statistical analysis of working days of each cytokines repetitive variance analysis technique was used. Results: The levels of IL-1β, IL-6 and TNF-α were the highest in the 3rd day and started to decrease on the 7th and 10th days. Conclusions: The cytokine levels of orthodontic force applied teeth in late adult rats are compatible with the levels of studies in young rats.

Assessment of tumor necrosis factor alpha (TNFα) and interleukin 6 level in gingival crevicular fluid during orthodontic tooth movement: a randomized split-mouth clinical trial

“…This finding was in accordance with that of Basaran ( 22 ) and Ozcun ( 23 ) who reported no significant alterations in the level of pro-inflammatory cytokines following initiation of orthodontic treatment. However, in the study by Kaya et al, levels of IL-6 and TNFα reached their maximum at three days in rats and then started to decrease from days seven and 10 onwards ( 18 ). Ren et al showed that the level of IL-6 and TNFα significantly increased at 24 hours, and during the linear phase of OTM, concentration of these cytokines decreased almost to the baseline level ( 24 ).…”

“…Knowledge about chemical biomarkers, which indicate changes in bone remodeling, inflammation and root resorption, can help determine the exact amount of load and its duration of application required for each tooth, select the most efficient treatment plan with minimal complications and accelerate the treatment course ( 17 ). Most studies ( 18 , 13 ) did not compare the level of cytokines at the tensile and pressure side, and in some studies ( 5 , 16 ) both sides were compared, but these studies reported contradictory results. Also, most studies ( 18 , 19 ) examined the cytokines level one day after force application.…”

“…Most studies ( 18 , 13 ) did not compare the level of cytokines at the tensile and pressure side, and in some studies ( 5 , 16 ) both sides were compared, but these studies reported contradictory results. Also, most studies ( 18 , 19 ) examined the cytokines level one day after force application. Thus, this study aimed to assess the level of IL-6 and TNFα and compare their levels at the pressure and tension sites shortly (one hour) after force application.…”