The gingival crevicular fluid Interleukin‐1β and tumour necrosis factor‐α levels in patients with rapidly progressive periodontitis

Yavuzyilmaz, Ezel; Yamalik, Nermin; Bulut, Serpil; Özen, Seza; Ersoy, F; Saatçi, U

doi:10.1111/j.1834-7819.1995.tb05614.x

Cited by 48 publications

(36 citation statements)

References 20 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, a correlation between the clinical parameters of periodontal lesions and levels of TNF-␣ in gingival tissue has been shown (19). Despite these observations, numerous reports undermine the significance of TNF-␣ involvement in the progression of periodontitis (9,16,46) and implicate IL-1 as the major factor responsible for the clinical outcome of this disease (14,45,52). The observation that IL-1␤ is present at significantly higher levels than TNF-␣ in GCF and inflamed periodontal tissue additionally supports this hypothesis (15,19,45,52).…”

Section: Resultsmentioning

confidence: 99%

Inactivation of Membrane Tumor Necrosis Factor Alpha by Gingipains fromPorphyromonas gingivalis

Mężyk-Kopeć¹,

Potempa

Bzowska³

et al. 2005

Infect Immun

View full text Add to dashboard Cite

Gingipains are cysteine proteinases produced by Porphyromonas gingivalis, a major causative bacterium of adult periodontitis. They consist of arginine-specific (HRgpA and RgpB) and lysine-specific (Kgp) proteinases. Gingipains strongly affect the host defense system by degrading some cytokines, components of the complement system, and several immune cell receptors. In an in vitro model, gingipains were shown to degrade soluble tumor necrosis factor alpha (TNF-␣). However, since membrane TNF-␣ shows strong biological activity, especially in local inflammatory lesions, it was worth investigating whether gingipains might also destroy membrane TNF-␣ and limit its biological activities. To avoid a possible influence of gingipains on ADAM17, the secretase of TNF-␣, the majority of experiments were performed using ADAM17 ؊/؊ fibroblasts stably transfected with cDNA of human pro-TNF-␣ (ADAM17 ؊/؊ TNF ؉ ). Arginine-specific gingipains (Rgp's) strongly diminished the level of TNF-␣ on the cell surface as measured by flow cytometry, and this process was not accompanied by an increased concentration of soluble TNF-␣ in the culture medium. Degradation of membrane TNF-␣ by Rgp's correlated with a strong decrease in TNF-␣-mediated biological activities of ADAM17 ؊/؊ TNF ؉ cells. First, the activation state of transcription factor NF-B was suppressed; second, the cells were no longer able to induce apoptosis in HL-60 cells. Kgp was also able to cleave membrane TNF-␣, but its effect was much weaker than that of Rgp's. Gingipains also limited the binding of native TNF-␣ to the target cells. Thus, gingipains are able not only to cleave soluble TNF-␣ but also to destroy the membrane form of the cytokine, which may additionally dysregulate the cytokine network.

show abstract

Section: Resultsmentioning

confidence: 99%

Inactivation of Membrane Tumor Necrosis Factor Alpha by Gingipains fromPorphyromonas gingivalis

Mężyk-Kopeć¹,

Potempa

Bzowska³

et al. 2005

Infect Immun

View full text Add to dashboard Cite

show abstract

“…In addition, the mean and median levels of IL-17, IL-6, and TNF-α in patients with ELANE mutations were higher than in HAX1 or unknown mutations although these differences did not reach statistical significance, most likely due to the small size of the cohort and great variations within groups. It is known that elevated levels of IL-1β [39, 40], IL-6 [41], TNF-α [42], and IL-17 [43] in GCF are associated with severe periodontal disease, and that these cytokines may also be elevated in chronic periodontitis tissue [44–46]. Thus, the GCF from patients with ELANE mutations displays the presence of the strong proinflammatory cytokine IL-1β, which might be expected in the inflamed periodontium.…”

Section: Discussionmentioning

confidence: 99%

Mutations in the ELANE Gene are Associated with Development of Periodontitis in Patients with Severe Congenital Neutropenia

Carlsson

Wondimu

et al. 2011

J Clin Immunol

View full text Add to dashboard Cite

BackgroundPatients with severe congenital neutropenia (SCN) often develop periodontitis despite standard medical and dental care. In light of previous findings that mutations in the neutrophil elastase gene, ELANE, are associated with more severe neutropenic phenotypes, we hypothesized an association between the genotype of SCN and development of periodontitis.MethodsFourteen Swedish patients with SCN or cyclic neutropenia harboring different genetic backgrounds were recruited for periodontal examination. Peripheral blood, gingival crevicular fluid (GCF), and subgingival bacterial samples were collected. The levels of cytokines and antibacterial peptides were determined in GCF and plasma by multiplex immunoassay and immunoblotting, respectively. Subgingival bacterial samples were analyzed using 16S rDNA pyrosequencing.ResultsELANE mutations correlated with more severe periodontal status than the HAX1 or unknown mutations in patients with SCN. The subjects with mutant ELANE had higher levels of IL-1β in GCF. Using principal coordinate analysis of the subgingival microbiota, patients with ELANE mutations and reference subjects with periodontitis tended to cluster differently from patients with HAX1 or unknown mutations and non-periodontitis reference subjects.ConclusionThis study demonstrates an association between ELANE mutations in SCN and the development of periodontitis with skewed subgingival microbiota, indicating a potential role of ELANE mutations in the pathogenesis of periodontitis.

show abstract

“…[72] The GCF isolated from tooth, 24 h after being subjected to orthodontic forces, showed an increase in the levels of IL-1β, IL-6, TNF-α, epidermal growth factor and β 2 -microglobulin, when compared to controls. [73] IL-8 was found to be associated with the bone remodeling process in orthodontic patients. [74] The level of pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α reached a significant level at 24 h after application of orthodontic forces.…”

Section: Gingival Crevicular Fluid During Orthodontic Tooth Movementmentioning

confidence: 99%

Biological response at the cellular level within the periodontal ligament on application of orthodontic force - An update

Meeran¹

2012

J Orthodont Sci

View full text Add to dashboard Cite

Orthodontic force elicits a biological response in the tissues surrounding the teeth, resulting in remodeling of the periodontal ligament and the alveolar bone. The force-induced tissue strain result in reorganization of both cellular and extracellular matrix, besides producing changes in the local vascularity. This in turn leads to the synthesis and release of various neurotransmitters, arachidonic acid, growth factors, metabolites, cytokines, colony-stimulating factors, and enzymes like cathepsin K, matrix metalloproteinases, and aspartate aminotransferase. Despite the availability of many studies in the orthodontic and related scientific literature, a concise integration of all data is still lacking. Such a consolidation of the rapidly accumulating scientific information should help in understanding the biological processes that underlie the phenomenon of tooth movement in response to mechanical loading. Therefore, the aim of this review was to describe the biological processes taking place at the molecular level on application of orthodontic force and to provide an update of the current literature.

show abstract

The gingival crevicular fluid Interleukin‐1β and tumour necrosis factor‐α levels in patients with rapidly progressive periodontitis

Cited by 48 publications

References 20 publications

Inactivation of Membrane Tumor Necrosis Factor Alpha by Gingipains fromPorphyromonas gingivalis

Inactivation of Membrane Tumor Necrosis Factor Alpha by Gingipains fromPorphyromonas gingivalis

Mutations in the ELANE Gene are Associated with Development of Periodontitis in Patients with Severe Congenital Neutropenia

Biological response at the cellular level within the periodontal ligament on application of orthodontic force - An update

Contact Info

Product

Resources

About