The gill sialic acid content as an index of environmental stress in rainbow trout, <i>Salmo gairdneri</i>, Richardson

Arillo, Attilio; Margiocco, C.; Melodia, F.

doi:10.1111/j.1095-8649.1979.tb03623.x

Cited by 24 publications

(8 citation statements)

References 11 publications

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“…Sialic acid has been histochemically localized to the contents of rainbow trout branchial goblet cells (Fletcher et al 1976;Ferguson et al 1992) and sialic acid has been used to measure mucus production (Arillo et al 1979;Eddy and Fraser 1982). The amount of sialic acid contained in isolated gill mucin is very low however (0.06 g/g mucin protein or approximately 0.035 gM/mg mucin dry weight).…”

Section: Discussionmentioning

confidence: 99%

Isolation and partial characterization of rainbow trout (Oncorhynchus mykiss) gill mucin

Lumsden

Ferguson

1994

Fish Physiol Biochem

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Gill mucin from rainbow trout was isolated utilizing two rounds of cesium chloride density ultracentrifugation followed by gel filtration on Sepharose CL-2B. Neither density ultracentrifugation nor gel filtration alone was sufficient for purification of the mucin. Isolated gill mucin had a density of 1.5 g/ml and eluted at the void volume of the Sepharose CL-2B column. Silver-stained reducing 6% polyacrylamide gel electrophoresis of gill mucin produced a band at the origin with a smear entering the separating gel. There was no evidence of a link protein in gill mucin on reducing 12% polyacrylamide gel electrophoresis. Gill mucin had an amino acid profile similar to that of mucins in other species. Specifically, 35.1% of the total amino acids were represented by threonine and serine, while another 27.5% were alanine and proline. Gill mucin contained galactose (26.7 ± 3.2%), galactosamine (22.5 ± 4.4%), glucose (16.6 ± 8.7%), fucose (16.1 ± 1.5%), glucosamine (12.0 ± 1.9%) and mannose (5.1 ± 4.4%). Uronic acid levels from purified mucin were very low (0.7 ± 0.1%). Sialic acid was also present (0.06 g/g of mucin protein). The periodic acid-Schiff assay routinely utilized for detection of mucins was relatively insensitive for detection of gill mucin (6 × less sensitive than for pig gastric mucin) so a rabbit antiserum was raised. The antiserum produced profiles similar to the periodic acid-Schiff assay of fractions following gel filtration. Immunofluorescence of formalin-fixed rainbow trout gill tissue sections showed that the antiserum detected mucin within branchial goblet cells.

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Section: Discussionmentioning

confidence: 99%

Isolation and partial characterization of rainbow trout (Oncorhynchus mykiss) gill mucin

Lumsden

Ferguson

1994

Fish Physiol Biochem

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“…Furthermore, they are involved in osmorregulation (Gonzales and McDonald, 1992;Flik and Verbost, 1993;Romão et al, 2001;Verbost et al, 1994), acid-base balance (Epstein et al, 1980;Evans et al, 1982;Lin and Randall, 1991;McDonald et al, 1991;Goss et al, 1992), excretion of nitrogenous compounds (Goldstein, 1982;Evans and Cameron, 1986;Sayer and Davenport, 1987), and taste (Hughes, 1982;Rios and Fanta, 1998). Organic Pesticides (Davis and Wedemeyer, 1971;Rao and Rao, 1981;Mallatt, 1985;Evans, 1987;Laurent and Perry, 1991;Nowak, 1992;Wendelaar Bonga and Lock, 1992), detergents (Schimid and Mann, 1961;Abel, 1976;Bolis and Rankin, 1980), acids (Daye and Garside, 1980;McDonald, 1983;Kawall, 1993), salts (Hossler, 1980;Luvizotto, 1994;Fanta et al, 1995), industrial waste (Mitz and Giesy, 1985;Stoker et al, 1985;Lindesjöö and Thulin, 1994), ammonia (Smart, 1976;Arillo et al, 1979;Soderberg et al, 1984) and heavy metals (Skidmore, 1970;Matthiessen and Brafield, 1973;Lock and ...…”

Section: *Loov Duh Ylwdo Vwuxfwxuhv Iru Ilvk Vlqfh Wkh\ Duh Wkh Pdlq mentioning

confidence: 99%

Effects of the organophosphorous methyl parathion on the branchial epithelium of a freshwater fish Metynnis roosevelti

Machado

Fanta

2003

Braz. arch. biol. technol.

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Gills are vital structures for fish, since they are the main site for gaseous exchange as well as partially responsible for osmorregulation, acid-basic balance, excretion of nitrogenous compounds and taste. Chemicals in the water may alter the morphology of branchial cells of fish that are, therefore, a useful model for environmental impact and ecotoxicology studies. In order to investigate the effects of an organophosphorous compound, methyl parathion, on the gills of the fish, samples of Metynnis roosevelti were exposed to lethal (7ppm) and sublethal (1ppm) doses of Mentox 600 CE. Through light and scanning electron microscopy, shrinking of the branchial epithelium, followed by detachment and hyperplasia were observed. Externally, the branchial filaments presented the gradual disappearance of microridges. Even in sublethal doses, the organophosphorous reduced the health and fitness of these fish, as consequence of secondary effects derived from changes in the branchial epithelium, impairing oxygenation and ionic balance of the organism.
As brânquias são estruturas vitais para peixes, pois são o principal local de trocas gasosas, assim como parcialmente responsáveis pela osmorregulação, pelo equilíbrio ácido-básico, pela excreção de compostos nitrogenados e pela gustação. Compostos químicos na água podem alterar a morfologia das células branquiais de peixes, que se constituem, dessa forma, em um útil modelo para estudos de impacto ambiental. Visando investigar os efeitos de um composto organofosforado, o metil paration, em brânquias de peixes, exemplares de Metynnis roosevelti foram expostos a doses letais (7ppm) e subletais (1ppm) de Mentox 600 CE. Observações em microscopia óptica e eletrônica de varredura evidenciaram enrugamento do epitélio branquial, seguido por descolamento e hiperplasia. Externamente, os filamentos branquiais apresentaram desaparecimento gradual das microssaliências. Os resultados mostram que, mesmo em doses subletais, o organofosforado poderá levar o animal a sofrer conseqüências secundárias decorrentes das alterações da superfície de trocas gasosas e iônicas

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“…Binding of N-acetyl neuraminic acid to the surface of the bacteria, in turn, may change the hydrophobicity of the bacterium. A number of reports have shown sialic acid to be found at sites where bacteria are thought to enter the fish, such as on the surface of their gills (Arillo et al, 1975), intes tines (Kulshresha and Jauhar, 1984) and skin (Mittal et al, 1994). The adherence of P. damselae subsp.…”

Section: Discussionmentioning

confidence: 99%

Determination of the Attachment of Photobacterium damselae subsp. piscicida to Fish Cells Using an Enzyme Linked Immunosorbent Assay.

Jung¹,

Thompson²,

Adams³

2001

Fish Pathol.

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The gill sialic acid content as an index of environmental stress in rainbow trout, Salmo gairdneri, Richardson

Cited by 24 publications

References 11 publications

Isolation and partial characterization of rainbow trout (Oncorhynchus mykiss) gill mucin

Isolation and partial characterization of rainbow trout (Oncorhynchus mykiss) gill mucin

Effects of the organophosphorous methyl parathion on the branchial epithelium of a freshwater fish Metynnis roosevelti

Determination of the Attachment of Photobacterium damselae subsp. piscicida to Fish Cells Using an Enzyme Linked Immunosorbent Assay.

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